Abstract
Southern blot analysis revealed a ubiquitous distribution and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.
Original language | English |
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Pages (from-to) | 318-327 |
Number of pages | 10 |
Journal | Molecules and cells |
Volume | 19 |
Issue number | 3 |
Publication status | Published - 2005 |
Keywords
- Genetic Diversity
- Hibiscus syriacus
- Integrase
- Phylogenetic Analysis
- gypsy-like Retrotransposon
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology