Abstract
Southern blot analysis revealed a ubiquitous distribution and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.
| Original language | English |
|---|---|
| Pages (from-to) | 318-327 |
| Number of pages | 10 |
| Journal | Molecules and Cells |
| Volume | 19 |
| Issue number | 3 |
| Publication status | Published - 2005 Dec 1 |
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Keywords
- Genetic Diversity
- gypsy-like Retrotransposon
- Hibiscus syriacus
- Integrase
- Phylogenetic Analysis
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology
Cite this
Characterization of Ty3-gypsy-like elements in Hibiscus syriacus. / Jeung, Ji Ung; Cho, Sung Ki; Lee, Seung Jae; Shin, Jeong Sheop.
In: Molecules and Cells, Vol. 19, No. 3, 01.12.2005, p. 318-327.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of Ty3-gypsy-like elements in Hibiscus syriacus
AU - Jeung, Ji Ung
AU - Cho, Sung Ki
AU - Lee, Seung Jae
AU - Shin, Jeong Sheop
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Southern blot analysis revealed a ubiquitous distribution and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.
AB - Southern blot analysis revealed a ubiquitous distribution and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.
KW - Genetic Diversity
KW - gypsy-like Retrotransposon
KW - Hibiscus syriacus
KW - Integrase
KW - Phylogenetic Analysis
UR - http://www.scopus.com/inward/record.url?scp=27244446655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27244446655&partnerID=8YFLogxK
M3 - Article
C2 - 15995347
AN - SCOPUS:27244446655
VL - 19
SP - 318
EP - 327
JO - Molecules and Cells
JF - Molecules and Cells
SN - 1016-8478
IS - 3
ER -