Abstract
Southern blot analysis revealed a ubiquitous distribution and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.
| Original language | English |
|---|---|
| Pages (from-to) | 318-327 |
| Number of pages | 10 |
| Journal | Molecules and Cells |
| Volume | 19 |
| Issue number | 3 |
| Publication status | Published - 2005 Dec 1 |
Keywords
- Genetic Diversity
- gypsy-like Retrotransposon
- Hibiscus syriacus
- Integrase
- Phylogenetic Analysis
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Cell Biology