Characterization of Ty3-gypsy-like elements in Hibiscus syriacus

Ji Ung Jeung, Sung Ki Cho, Seung Jae Lee, Jeong Sheop Shin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Southern blot analysis revealed a ubiquitous distribution and high copy number of Ty3-gypsy-like elements in the genome of Hibiscus syriacus. Comparative phylogenetic analysis of the large subunit of Rubisco and the integrase region of Ty3-gypsy elements in various plant species indicated that the retrotransposon-like sequences have different evolutionary histories and their own unique polymorphism in the H. syriacus population. Sequence-tagged site-restriction fragment length polymorphisms (STS-RFLP) analysis also indicated great variability in the numbers and sequences of Ty3-gypsy-like elements within H. syriacus varieties. Ty3-gypsy-like elements may still be active within H. syriacus, since Northern analysis of wounded leaves of H. syriacus variety Saehan with a probe for the integrase domain gave strong hybridization signals. The sequence heterogeneity and ubiquity of the Ty3-gypsy-like elements in H. syriacus genomes could provide reliable DNA markers for line identification as well for the analysis of genetic diversity in H. syriacus.

Original languageEnglish
Pages (from-to)318-327
Number of pages10
JournalMolecules and Cells
Volume19
Issue number3
Publication statusPublished - 2005 Dec 1

Keywords

  • Genetic Diversity
  • gypsy-like Retrotransposon
  • Hibiscus syriacus
  • Integrase
  • Phylogenetic Analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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