Characterizations of Pneumocystis carinii and rat lung lipids

Glyceryl ethers and fatty alcohols

Edna S. Kaneshiro, Zengkui Guo, Dong Geun Sul, Kathryn A. Kallam, Koka Jayasimhulu, David H. Beach

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pneumocystis carinii carinii and rat lung phospholipids contained 3-6% 1-alkyl-2-acyl glycerols composed of the glyceryl ether species, 1-O- octadecyl glycerol (batyl alcohol), 1-O-octadec-9-enyl glycerol (selachyl alcohol), 1-O-hexadecyl glycerol (chimyl alcohol), and 1-O-hexadec-9-enyl glycerol. Of the major phospholipid classes, phosphatidylinositol (PI) and phosphatidylserine contained the highest percentage of alkyl acyl glycerols. Methylprednisolone treatment caused an increase in alkyl acyl PI of rat lung lipids from 12% to 45%., As the PI concentration in lung phospholipids increases in rats treated with methylprednisolone, the increase in alkyl acyl PI was substantial; the proportions of alkyl acyl phosphatidylethanolamine and alkyl acyl lyso phosphatidylcholine (PC) also increased. Pneumocystis phospholipids contained higher proportions of alkyl acyl PC than the phospholipids of the lungs from normal and immunosuppressed uninfected rats. The glyceryl ether compositions of P. carinii carinii PC and lyso PC were similar, which suggests that lyso PC in the organism is derived by phospholipase A2 action on PC. This was not the case for PC and lyso PC of the lung controls. Analysis of the free fatty alcohols, precursors of glyceryl ethers identified only saturated species in P. carinii carinii and rat lung controls. Thus, the introduction of a double bond in the alcohol moiety of glyceryl ethers occurs after formation of the ether linkage between fatty alcohol and the glyceryl backbone. - Kaneshiro, E. S., Z. Guo, D. Sul, K. A. Kallam, K. Jayasimhulu, and D. H. Beach. Characterization of Pneumocystis carinii and rat lung lipids: glyceryl ethers and fatty alcohols.

Original languageEnglish
Pages (from-to)1907-1917
Number of pages11
JournalJournal of Lipid Research
Volume39
Issue number10
Publication statusPublished - 1998 Oct 1
Externally publishedYes

Fingerprint

Glyceryl Ethers
Fatty Alcohols
Pneumocystis carinii
Phosphatidylcholines
Rats
Glycerol
Lipids
Lung
Phospholipids
Phosphatidylinositols
Methylprednisolone
Pneumocystis
Phospholipases A2
Phosphatidylserines
Ether
Alcohols

Keywords

  • 1-O-hexadec-9- enyl glycerol
  • 1-O-octadec-9-enyl glycerol
  • AIDS
  • Batyl alcohol
  • Chimyl alcohol
  • Long chain alcohols
  • Phospholipids

ASJC Scopus subject areas

  • Endocrinology

Cite this

Kaneshiro, E. S., Guo, Z., Sul, D. G., Kallam, K. A., Jayasimhulu, K., & Beach, D. H. (1998). Characterizations of Pneumocystis carinii and rat lung lipids: Glyceryl ethers and fatty alcohols. Journal of Lipid Research, 39(10), 1907-1917.

Characterizations of Pneumocystis carinii and rat lung lipids : Glyceryl ethers and fatty alcohols. / Kaneshiro, Edna S.; Guo, Zengkui; Sul, Dong Geun; Kallam, Kathryn A.; Jayasimhulu, Koka; Beach, David H.

In: Journal of Lipid Research, Vol. 39, No. 10, 01.10.1998, p. 1907-1917.

Research output: Contribution to journalArticle

Kaneshiro, ES, Guo, Z, Sul, DG, Kallam, KA, Jayasimhulu, K & Beach, DH 1998, 'Characterizations of Pneumocystis carinii and rat lung lipids: Glyceryl ethers and fatty alcohols', Journal of Lipid Research, vol. 39, no. 10, pp. 1907-1917.
Kaneshiro ES, Guo Z, Sul DG, Kallam KA, Jayasimhulu K, Beach DH. Characterizations of Pneumocystis carinii and rat lung lipids: Glyceryl ethers and fatty alcohols. Journal of Lipid Research. 1998 Oct 1;39(10):1907-1917.
Kaneshiro, Edna S. ; Guo, Zengkui ; Sul, Dong Geun ; Kallam, Kathryn A. ; Jayasimhulu, Koka ; Beach, David H. / Characterizations of Pneumocystis carinii and rat lung lipids : Glyceryl ethers and fatty alcohols. In: Journal of Lipid Research. 1998 ; Vol. 39, No. 10. pp. 1907-1917.
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abstract = "Pneumocystis carinii carinii and rat lung phospholipids contained 3-6{\%} 1-alkyl-2-acyl glycerols composed of the glyceryl ether species, 1-O- octadecyl glycerol (batyl alcohol), 1-O-octadec-9-enyl glycerol (selachyl alcohol), 1-O-hexadecyl glycerol (chimyl alcohol), and 1-O-hexadec-9-enyl glycerol. Of the major phospholipid classes, phosphatidylinositol (PI) and phosphatidylserine contained the highest percentage of alkyl acyl glycerols. Methylprednisolone treatment caused an increase in alkyl acyl PI of rat lung lipids from 12{\%} to 45{\%}., As the PI concentration in lung phospholipids increases in rats treated with methylprednisolone, the increase in alkyl acyl PI was substantial; the proportions of alkyl acyl phosphatidylethanolamine and alkyl acyl lyso phosphatidylcholine (PC) also increased. Pneumocystis phospholipids contained higher proportions of alkyl acyl PC than the phospholipids of the lungs from normal and immunosuppressed uninfected rats. The glyceryl ether compositions of P. carinii carinii PC and lyso PC were similar, which suggests that lyso PC in the organism is derived by phospholipase A2 action on PC. This was not the case for PC and lyso PC of the lung controls. Analysis of the free fatty alcohols, precursors of glyceryl ethers identified only saturated species in P. carinii carinii and rat lung controls. Thus, the introduction of a double bond in the alcohol moiety of glyceryl ethers occurs after formation of the ether linkage between fatty alcohol and the glyceryl backbone. - Kaneshiro, E. S., Z. Guo, D. Sul, K. A. Kallam, K. Jayasimhulu, and D. H. Beach. Characterization of Pneumocystis carinii and rat lung lipids: glyceryl ethers and fatty alcohols.",
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