Characterizing DNA methylation patterns in pancreatic cancer genome

Aik Choon Tan, Antonio Jimeno, Steven H. Lin, Jenna Wheelhouse, Fonda Chan, Anna Solomon, N. V. Rajeshkumar, Belen Rubio-Viqueira, Manuel Hidalgo

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

We performed a global methylation profiling assay on 1505 CpG sites across 807 genes to characterize DNA methylation patterns in pancreatic cancer genome. We found 289 CpG sites that were differentially methylated in normal pancreas, pancreatic tumors and cancer cell lines. We identified 23 and 35 candidate genes that are regulated by hypermethylation and hypomethylation in pancreatic cancer, respectively. We also identified candidate methylation markers that alter the expression of genes critical to gemcitabine susceptibility in pancreatic cancer. These results indicate that aberrant DNA methylation is a frequent epigenetic event in pancreatic cancer; and by using global methylation profiling assay, it is possible to identify these markers for diagnostic and therapeutic purposes in this disease.

Original languageEnglish
Pages (from-to)425-438
Number of pages14
JournalMolecular Oncology
Volume3
Issue number5-6
DOIs
Publication statusPublished - 2009 Dec

Keywords

  • DNA methylation
  • Gemcitabine
  • Gene expression
  • Hypermethylation
  • Hypomethylation
  • Pancreatic cancer

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Oncology
  • Cancer Research

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  • Cite this

    Tan, A. C., Jimeno, A., Lin, S. H., Wheelhouse, J., Chan, F., Solomon, A., Rajeshkumar, N. V., Rubio-Viqueira, B., & Hidalgo, M. (2009). Characterizing DNA methylation patterns in pancreatic cancer genome. Molecular Oncology, 3(5-6), 425-438. https://doi.org/10.1016/j.molonc.2009.03.004