Chatacterization of GCN4 mutants with mormal dna binding activities and decreased transcriptional activation abilities

Joon Kim, Jae Yung Lee, Seong Joong Kim

Research output: Contribution to journalArticle

Abstract

The (R_'N4 protein, a transcriptional activator in yeast, belongs Io the AP-1 family of transcription factors and binds to the same )-1 sexluence, ATGACTCAT. us mammalian)-1 protein and Jun cncoprotein via a bZIP domain. We develotx'd a screening system which can isolate ( ;CN4 derivatives with mutations in the DNA binding domain. In orr to identify mnino acids in (FCN4 protein that plays a rnle in tJrotein-protein inmraction, we ix'r formed saturation mutagenesis in the I)NA binding)main of GCN'I using oligonueleotides containing randomized ccxton bases. The mutants were assayed for their ability to sulort transcril)tional activation, binding activity by chevking sensitivity of 3-aminotriazol, and in vitro binding assay. Two of the mutants have a normal binding activity with decreased tranrilJtional activation ability. The residues identified in these mutants may Ix: involved in the interaction with other proteins for GCN activation.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number9
Publication statusPublished - 1997 Dec 1

Fingerprint

transcriptional activation
Transcriptional Activation
Chemical activation
mutants
Proteins
proteins
Mutagenesis
DNA-binding domains
Transcription Factor AP-1
mutagenesis
Yeast
Assays
Screening
Transcription Factors
transcription factors
Yeasts
chemical derivatives
yeasts
screening
Derivatives

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Chatacterization of GCN4 mutants with mormal dna binding activities and decreased transcriptional activation abilities. / Kim, Joon; Lee, Jae Yung; Kim, Seong Joong.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997.

Research output: Contribution to journalArticle

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