Chemerin Expression in the Peritoneal Fluid, Serum, and Ovarian Endometrioma of Women with Endometriosis

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9 Citations (Scopus)

Abstract

Problem: Inflammation is an essential process in the pathogenesis of endometriosis. Method of study: Serum and peritoneal fluid (PF) samples were collected from women with endometriosis (n = 31) and women without endometriosis (n = 48). Chemerin, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) levels in serum and PF samples were determined with a specific enzyme-linked immunosorbent assay. Eutopic endometrial tissue from controls and ovarian endometriotic cysts were obtained during surgery. Expression of chemerin and chemerin receptors in ectopic and eutopic endometrial tissues was measured on real-time reverse transcriptase-polymerase chain reaction. Protein expression was examined with Western blot and densitometric analysis. Results: Chemerin concentrations were higher in PF from women with endometriosis than that in that from controls. PF chemerin concentrations were significantly correlated with both TNF-α and IL-6 levels in PF. The mRNA and protein of chemerin and its receptor were significantly increased in the ovarian endometrioma tissue compared with eutopic endometrium of controls. Conclusion: These findings suggest that chemerin plays a role in endometriosis-related pelvic inflammation.

Original languageEnglish
Pages (from-to)379-386
Number of pages8
JournalAmerican Journal of Reproductive Immunology
Volume74
Issue number4
DOIs
Publication statusPublished - 2015 Oct 1

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Ascitic Fluid
Endometriosis
Serum
Interleukin-6
Tumor Necrosis Factor-alpha
Inflammation
Ovarian Cysts
Endometrium
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Proteins
Western Blotting
Enzyme-Linked Immunosorbent Assay
Messenger RNA

Keywords

  • Chemerin
  • Endometriosis
  • Inflammation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

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title = "Chemerin Expression in the Peritoneal Fluid, Serum, and Ovarian Endometrioma of Women with Endometriosis",
abstract = "Problem: Inflammation is an essential process in the pathogenesis of endometriosis. Method of study: Serum and peritoneal fluid (PF) samples were collected from women with endometriosis (n = 31) and women without endometriosis (n = 48). Chemerin, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) levels in serum and PF samples were determined with a specific enzyme-linked immunosorbent assay. Eutopic endometrial tissue from controls and ovarian endometriotic cysts were obtained during surgery. Expression of chemerin and chemerin receptors in ectopic and eutopic endometrial tissues was measured on real-time reverse transcriptase-polymerase chain reaction. Protein expression was examined with Western blot and densitometric analysis. Results: Chemerin concentrations were higher in PF from women with endometriosis than that in that from controls. PF chemerin concentrations were significantly correlated with both TNF-α and IL-6 levels in PF. The mRNA and protein of chemerin and its receptor were significantly increased in the ovarian endometrioma tissue compared with eutopic endometrium of controls. Conclusion: These findings suggest that chemerin plays a role in endometriosis-related pelvic inflammation.",
keywords = "Chemerin, Endometriosis, Inflammation",
author = "Jin, {Chan Hee} and Yi, {Kyong Wook} and Ha, {Young Ran} and Jung-Ho Shin and Hyun-Tae Park and Tak Kim and Hur, {Jun Young}",
year = "2015",
month = "10",
day = "1",
doi = "10.1111/aji.12405",
language = "English",
volume = "74",
pages = "379--386",
journal = "American Journal of Reproductive Immunology",
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T1 - Chemerin Expression in the Peritoneal Fluid, Serum, and Ovarian Endometrioma of Women with Endometriosis

AU - Jin, Chan Hee

AU - Yi, Kyong Wook

AU - Ha, Young Ran

AU - Shin, Jung-Ho

AU - Park, Hyun-Tae

AU - Kim, Tak

AU - Hur, Jun Young

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Problem: Inflammation is an essential process in the pathogenesis of endometriosis. Method of study: Serum and peritoneal fluid (PF) samples were collected from women with endometriosis (n = 31) and women without endometriosis (n = 48). Chemerin, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) levels in serum and PF samples were determined with a specific enzyme-linked immunosorbent assay. Eutopic endometrial tissue from controls and ovarian endometriotic cysts were obtained during surgery. Expression of chemerin and chemerin receptors in ectopic and eutopic endometrial tissues was measured on real-time reverse transcriptase-polymerase chain reaction. Protein expression was examined with Western blot and densitometric analysis. Results: Chemerin concentrations were higher in PF from women with endometriosis than that in that from controls. PF chemerin concentrations were significantly correlated with both TNF-α and IL-6 levels in PF. The mRNA and protein of chemerin and its receptor were significantly increased in the ovarian endometrioma tissue compared with eutopic endometrium of controls. Conclusion: These findings suggest that chemerin plays a role in endometriosis-related pelvic inflammation.

AB - Problem: Inflammation is an essential process in the pathogenesis of endometriosis. Method of study: Serum and peritoneal fluid (PF) samples were collected from women with endometriosis (n = 31) and women without endometriosis (n = 48). Chemerin, tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) levels in serum and PF samples were determined with a specific enzyme-linked immunosorbent assay. Eutopic endometrial tissue from controls and ovarian endometriotic cysts were obtained during surgery. Expression of chemerin and chemerin receptors in ectopic and eutopic endometrial tissues was measured on real-time reverse transcriptase-polymerase chain reaction. Protein expression was examined with Western blot and densitometric analysis. Results: Chemerin concentrations were higher in PF from women with endometriosis than that in that from controls. PF chemerin concentrations were significantly correlated with both TNF-α and IL-6 levels in PF. The mRNA and protein of chemerin and its receptor were significantly increased in the ovarian endometrioma tissue compared with eutopic endometrium of controls. Conclusion: These findings suggest that chemerin plays a role in endometriosis-related pelvic inflammation.

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