TY - JOUR
T1 - Chemical Constituents from the Aerial Parts of Elsholtzia ciliata and Their Protective Activities on Glutamate-Induced HT22 Cell Death
AU - Seo, Young Hye
AU - Trinh, Tuy An
AU - Ryu, Seung Mok
AU - Kim, Hyo Seon
AU - Choi, Goya
AU - Moon, Byeong Cheol
AU - Shim, Sang Hee
AU - Jang, Dae Sik
AU - Lee, Dongho
AU - Kang, Ki Sung
AU - Lee, Jun
N1 - Funding Information:
This work was supported by a National Research Council of Science & Technology (NST) grant by the Korean government (MSIP) (no. CRC-15-04-KIST, G17290) and the projects entitled “Applicational Development of Standardized Herbal Resources” and “Development of Sustainable Application for Standard Herbal Resources” (grant nos. KSN1911420 and KSN2012320) from the KIOM. This study was also supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT), Republic of Korea (grant no. 2019R1F1A1059173).
Publisher Copyright:
©
PY - 2020/10/23
Y1 - 2020/10/23
N2 - A new phenolic glucoside, (7E,9E)-3-hydroxyavenalumic acid-3-O-[6′-O-(E)-caffeoyl]-β-d-glucopyranoside (1), and three new acetylated flavone glycosides, acacetin-7-O-[β-d-glucopyranosyl(1″″→2″)-4′-O-acetyl-α-l-rhamnopyranosyl(1′→6″)]-β-d-glucopyranoside (3), acacetin-7-O-[6″″-O-acetyl-β-d-glucopyranosyl(1″″→2″)-3′-O-acetyl-α-l-rhamnopyranosyl(1′→6″)]-β-d-glucopyranoside (5), and acacetin-7-O-[3″″,6″″-di-O-acetyl-β-d-glucopyranosyl(1″″→2″)-4′-O-acetyl-α-l-rhamnopyranosyl(1′→6″)]-β-d-glucopyranoside (7), as well as 34 known compounds (2, 4, 6, and 8-38) were isolated from the aerial parts of Elsholtzia ciliata. The chemical structures of the new compounds were determined by spectroscopic/spectrometric data interpretation using NMR and HRESIMS. The neuroprotective effect of the isolated compounds was evaluated by a cell viability assay on HT22 murine hippocampal neuronal cells. Among them, 23 compounds, including new substances 1 and 3, exhibited neuroprotective effects against glutamate-induced HT22 cell death. In particular, compounds 2, 16, 17, 20, 22, 28, 29, and 31 presented potent neuroprotective effects with EC50 values of 1.5-8.3 μM.
AB - A new phenolic glucoside, (7E,9E)-3-hydroxyavenalumic acid-3-O-[6′-O-(E)-caffeoyl]-β-d-glucopyranoside (1), and three new acetylated flavone glycosides, acacetin-7-O-[β-d-glucopyranosyl(1″″→2″)-4′-O-acetyl-α-l-rhamnopyranosyl(1′→6″)]-β-d-glucopyranoside (3), acacetin-7-O-[6″″-O-acetyl-β-d-glucopyranosyl(1″″→2″)-3′-O-acetyl-α-l-rhamnopyranosyl(1′→6″)]-β-d-glucopyranoside (5), and acacetin-7-O-[3″″,6″″-di-O-acetyl-β-d-glucopyranosyl(1″″→2″)-4′-O-acetyl-α-l-rhamnopyranosyl(1′→6″)]-β-d-glucopyranoside (7), as well as 34 known compounds (2, 4, 6, and 8-38) were isolated from the aerial parts of Elsholtzia ciliata. The chemical structures of the new compounds were determined by spectroscopic/spectrometric data interpretation using NMR and HRESIMS. The neuroprotective effect of the isolated compounds was evaluated by a cell viability assay on HT22 murine hippocampal neuronal cells. Among them, 23 compounds, including new substances 1 and 3, exhibited neuroprotective effects against glutamate-induced HT22 cell death. In particular, compounds 2, 16, 17, 20, 22, 28, 29, and 31 presented potent neuroprotective effects with EC50 values of 1.5-8.3 μM.
UR - http://www.scopus.com/inward/record.url?scp=85094219694&partnerID=8YFLogxK
U2 - 10.1021/acs.jnatprod.0c00756
DO - 10.1021/acs.jnatprod.0c00756
M3 - Article
C2 - 32991171
AN - SCOPUS:85094219694
SN - 0163-3864
VL - 83
SP - 3149
EP - 3155
JO - Journal of Natural Products
JF - Journal of Natural Products
IS - 10
ER -