Chemical constituents isolated from Antarctic marine-derived Aspergillus sp. SF-5976 and their anti-inflammatory effects in LPS-stimulated RAW 264.7 and BV2 cells

Jaeyoung Kwon, Hyaemin Lee, Wonmin Ko, Dong Cheol Kim, Kwan Woo Kim, Hak Cheol Kwon, Yuanqiang Guo, Jae Hak Sohn, Joung Han Yim, Youn Chul Kim, Hyuncheol Oh, Dongho Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Three new benzaldehydes (1–3) and two new dioxopiperazine alkaloids (4 and 5), along with 23 known constituents, were isolated from Antarctic marine-derived Aspergillus sp. SF-5976. Their structures were determined by spectroscopic and chemical methods. Among them, 20 compounds showed inhibitory effects toward lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages with IC50 values of 7.3–77.4 μM, while 22 compounds did in BV2 microglia with those of 4.6–72.3 μM. Furthermore, the effects of the newly isolated compounds on LPS-stimulated inducible NO synthase (iNOS) expression were investigated, and 1–3, and 5 inhibited iNOS in RAW 264.7 cells, while 1–5 did in BV2 cells. Also, 1–3 and 5 inhibited LPS-induced prostaglandin E2 production with IC50 values of 9.1–66.8 μM in RAW 264.7 macrophages by suppressing cyclooxygenase-2 (COX-2), while in BV2 microglia, 1–5 showed activities with those of 2.8–49.4 μM.

Original languageEnglish
Pages (from-to)3905-3912
Number of pages8
JournalTetrahedron
Volume73
Issue number27-28
DOIs
Publication statusPublished - 2017 Jul 6

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Aspergillus
Lipopolysaccharides
Anti-Inflammatory Agents
Macrophages
Microglia
Inhibitory Concentration 50
Benzaldehydes
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Dinoprostone
Alkaloids
Nitric Oxide Synthase
Nitric Oxide
RAW 264.7 Cells

Keywords

  • Antarctic marine
  • Anti-inflammation
  • Aspergillus sp.
  • Circular dichroism

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

Cite this

Chemical constituents isolated from Antarctic marine-derived Aspergillus sp. SF-5976 and their anti-inflammatory effects in LPS-stimulated RAW 264.7 and BV2 cells. / Kwon, Jaeyoung; Lee, Hyaemin; Ko, Wonmin; Kim, Dong Cheol; Kim, Kwan Woo; Kwon, Hak Cheol; Guo, Yuanqiang; Sohn, Jae Hak; Yim, Joung Han; Kim, Youn Chul; Oh, Hyuncheol; Lee, Dongho.

In: Tetrahedron, Vol. 73, No. 27-28, 06.07.2017, p. 3905-3912.

Research output: Contribution to journalArticle

Kwon, Jaeyoung ; Lee, Hyaemin ; Ko, Wonmin ; Kim, Dong Cheol ; Kim, Kwan Woo ; Kwon, Hak Cheol ; Guo, Yuanqiang ; Sohn, Jae Hak ; Yim, Joung Han ; Kim, Youn Chul ; Oh, Hyuncheol ; Lee, Dongho. / Chemical constituents isolated from Antarctic marine-derived Aspergillus sp. SF-5976 and their anti-inflammatory effects in LPS-stimulated RAW 264.7 and BV2 cells. In: Tetrahedron. 2017 ; Vol. 73, No. 27-28. pp. 3905-3912.
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