Chemical genetics approach reveals importance of cAMP and MAP kinase signaling to lipid and carotenoid biosynthesis in microalgae

Yoon-E Choi, Jin Kyu Rhee, Hyun Soo Kim, Joon Woo Ahn, Hyemin Hwang, Ji Won Yang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this study, we attempted to understand signaling pathways behind lipid biosynthesis by employing a chemical genetics approach based on small molecule inhibitors. Specific signaling inhibitors of MAP kinase or modulators of cAMP signaling were selected to evaluate the functional roles of each of the key signaling pathways in three different microalgal species: Chlamydomonas reinhardtii, Chlorella vulgaris, and Haematococcus pluvialis. Our results clearly indicate that cAMP signaling pathways are indeed positively associated with microalgal lipid biosynthesis. In contrast, MAP kinase pathways in three microalgal species are all negatively implicated in both lipid and carotenoid biosynthesis.

Original languageEnglish
Pages (from-to)637-647
Number of pages11
JournalJournal of microbiology and biotechnology
Volume25
Issue number5
DOIs
Publication statusPublished - 2015 Jan 1

Keywords

  • CAMP signaling
  • Carotenoid biosynthesis
  • Lipid biosynthesis
  • MAP kinase
  • Microalgal signaling pathways

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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