Chemical properties and immuno-stimulating activities of crude polysaccharides from enzyme digests of tea leaves

Hye Ryung Park, Hyung Joo Suh, Kwang Won Yu, Tae Young Kim, Kwang Soon Shin

Research output: Contribution to journalArticle

Abstract

In order to develop new immuno-stimulating ingredients from mature leaves of green tea, crude polysaccharides were isolated from pectinase digests of tea leaves (green tea enzyme digestion, GTE-0), after which their immuno-stimulating activities and chemical properties were examined. GTE-0 mainly contained neutral sugars (54.9%) such as glucose (14.2%), arabinose (12.2%), rhamnose (11.1%), and galacturonic acid (45.1%), which are characteristic of pectic polysaccharides. The anti-complementary activity of GTE-0 was similar to that of polysaccharide K (used as positive control). Number of morphologically activated macrophages was significantly increased in the GTE-0-treated group. GTE-0 significantly augmented H2O2 and reactive oxygen species production by murine peritoneal macrophage cells in a dose-dependent manner, whereas production of nitric oxide showed the highest activity at a dose of 100 μg/mL among all tested concentrations. Murine peritoneal macrophages stimulated with GTE-0 showed enhanced production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factors-α in a dose-dependent manner. Further, GTE-0 induced higher phagocytic activity in a dose-dependent manner. In ex vivo assay for cytolytic activity of murine peritoneal macrophages, GTE-0-treated group showed significantly higher activity compared to the untreated group at an effector-to-target cell ratio of 20. The above results lead us to conclude that polysaccharides from leaves of green tea have a potent immuno-stimulating effect on murine peritoneal macrophage cells.

Original languageEnglish
Pages (from-to)664-672
Number of pages9
JournalJournal of the Korean Society of Food Science and Nutrition
Volume44
Issue number5
DOIs
Publication statusPublished - 2015 May 1

Fingerprint

Peritoneal Macrophages
Tea
tea
Polysaccharides
physicochemical properties
macrophages
polysaccharides
green tea
Enzymes
enzymes
mice
leaves
dosage
Tumor Necrosis Factors
Polygalacturonase
Rhamnose
Arabinose
Interleukin-12
galacturonic acid
rhamnose

Keywords

  • Complement
  • Cytokine
  • Green tea
  • Macrophage
  • Polysaccharide

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science

Cite this

Chemical properties and immuno-stimulating activities of crude polysaccharides from enzyme digests of tea leaves. / Park, Hye Ryung; Suh, Hyung Joo; Yu, Kwang Won; Kim, Tae Young; Shin, Kwang Soon.

In: Journal of the Korean Society of Food Science and Nutrition, Vol. 44, No. 5, 01.05.2015, p. 664-672.

Research output: Contribution to journalArticle

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