Abstract
The recombinant bioluminescent bacterium, DNT5, containing a nagR-nagAa::luxCDABE fusion, was tested in a multi-channel continuous monitoring system to evaluate its ability to detect benzoic acid derivatives. Seven chemicals, benzoic acid, salicylic acid, 2,5-dihydroxy benzoic acid, 3,5-dihydroxy benzoic acid, benzene, naphthalene and phenol, were used to characterize the responses of DNT5. This strain responded uniquely to each chemical, and these responses were then evaluated based upon the structures of each chemical. The greatest bioluminescent responses were to salicylic acid and benzoic acid, followed by 2,5-dihydroxy benzoic acid and 3,5-dihydroxy benzoic acid, but DNT5 was unresponsive when exposed to benzene, phenol and naphthalene, suggesting it has a strong preference for benzoic acid derivatives with few or no ring-substituted groups.
| Original language | English |
|---|---|
| Pages (from-to) | 330-334 |
| Number of pages | 5 |
| Journal | Journal of Biotechnology |
| Volume | 131 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2007 Sep 15 |
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Keywords
- Benzoic acid derivatives
- Chemical-specific biomonitoring
- Continuous monitoring
- Multi-channel continuous monitoring system
- nagR promoter
- Recombinant bioluminescent bacterium
ASJC Scopus subject areas
- Biotechnology
Cite this
Chemical-specific continuous biomonitoring using a recombinant bioluminescent bacterium DNT5 (nagR-nagAa : :luxCDABE). / Lee, Jin Hyung; Mitchell, Robert J.; Gu, Man Bock.
In: Journal of Biotechnology, Vol. 131, No. 3, 15.09.2007, p. 330-334.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemical-specific continuous biomonitoring using a recombinant bioluminescent bacterium DNT5 (nagR-nagAa
T2 - :luxCDABE)
AU - Lee, Jin Hyung
AU - Mitchell, Robert J.
AU - Gu, Man Bock
PY - 2007/9/15
Y1 - 2007/9/15
N2 - The recombinant bioluminescent bacterium, DNT5, containing a nagR-nagAa::luxCDABE fusion, was tested in a multi-channel continuous monitoring system to evaluate its ability to detect benzoic acid derivatives. Seven chemicals, benzoic acid, salicylic acid, 2,5-dihydroxy benzoic acid, 3,5-dihydroxy benzoic acid, benzene, naphthalene and phenol, were used to characterize the responses of DNT5. This strain responded uniquely to each chemical, and these responses were then evaluated based upon the structures of each chemical. The greatest bioluminescent responses were to salicylic acid and benzoic acid, followed by 2,5-dihydroxy benzoic acid and 3,5-dihydroxy benzoic acid, but DNT5 was unresponsive when exposed to benzene, phenol and naphthalene, suggesting it has a strong preference for benzoic acid derivatives with few or no ring-substituted groups.
AB - The recombinant bioluminescent bacterium, DNT5, containing a nagR-nagAa::luxCDABE fusion, was tested in a multi-channel continuous monitoring system to evaluate its ability to detect benzoic acid derivatives. Seven chemicals, benzoic acid, salicylic acid, 2,5-dihydroxy benzoic acid, 3,5-dihydroxy benzoic acid, benzene, naphthalene and phenol, were used to characterize the responses of DNT5. This strain responded uniquely to each chemical, and these responses were then evaluated based upon the structures of each chemical. The greatest bioluminescent responses were to salicylic acid and benzoic acid, followed by 2,5-dihydroxy benzoic acid and 3,5-dihydroxy benzoic acid, but DNT5 was unresponsive when exposed to benzene, phenol and naphthalene, suggesting it has a strong preference for benzoic acid derivatives with few or no ring-substituted groups.
KW - Benzoic acid derivatives
KW - Chemical-specific biomonitoring
KW - Continuous monitoring
KW - Multi-channel continuous monitoring system
KW - nagR promoter
KW - Recombinant bioluminescent bacterium
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UR - http://www.scopus.com/inward/citedby.url?scp=34548396389&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2007.06.020
DO - 10.1016/j.jbiotec.2007.06.020
M3 - Article
C2 - 17716769
AN - SCOPUS:34548396389
VL - 131
SP - 330
EP - 334
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 3
ER -