Chemical-specific continuous biomonitoring using a recombinant bioluminescent bacterium DNT5 (nagR-nagAa: :luxCDABE)

Jin Hyung Lee, Robert J. Mitchell, Man Bock Gu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The recombinant bioluminescent bacterium, DNT5, containing a nagR-nagAa::luxCDABE fusion, was tested in a multi-channel continuous monitoring system to evaluate its ability to detect benzoic acid derivatives. Seven chemicals, benzoic acid, salicylic acid, 2,5-dihydroxy benzoic acid, 3,5-dihydroxy benzoic acid, benzene, naphthalene and phenol, were used to characterize the responses of DNT5. This strain responded uniquely to each chemical, and these responses were then evaluated based upon the structures of each chemical. The greatest bioluminescent responses were to salicylic acid and benzoic acid, followed by 2,5-dihydroxy benzoic acid and 3,5-dihydroxy benzoic acid, but DNT5 was unresponsive when exposed to benzene, phenol and naphthalene, suggesting it has a strong preference for benzoic acid derivatives with few or no ring-substituted groups.

Original languageEnglish
Pages (from-to)330-334
Number of pages5
JournalJournal of Biotechnology
Volume131
Issue number3
DOIs
Publication statusPublished - 2007 Sep 15

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Keywords

  • Benzoic acid derivatives
  • Chemical-specific biomonitoring
  • Continuous monitoring
  • Multi-channel continuous monitoring system
  • nagR promoter
  • Recombinant bioluminescent bacterium

ASJC Scopus subject areas

  • Biotechnology

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