Chemopreventive Properties of Genipin on AGS Cell Line via Induction of JNK/Nrf2/ARE Signaling Pathway

Jee Min Kim, Hyeonseok Ko, Sun Joong Kim, So Hee Shim, Chang Hoon Ha, Hyo-Ihl Chang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Roles of dietary phytochemicals in cancer chemoprevention via induction of nuclear factor-erythroid-2-related factor 2 (Nrf2)-mediated antioxidant enzymes have been well established in a number of studies. In this study, FACS analysis was used to reveal that the intracellular reactive oxygen species level decreased at 0-25 μM of genipin treatment. Furthermore, immunofluorescence analysis and Western blotting were used to demonstrate that genipin treatment resulted in the upregulation and nuclear translocation of Nrf2, as well as upregulation of gastrointestinal glutathione peroxidase. Finally, we found that C-Jun-NH2-kinase (JNK) was also dose-dependently activated, where depleting JNK by using a biochemical inhibitor indicated that JNK was upstream of Nrf2. Interestingly, the antioxidant effects were limited to the treatment in the lower dosage of genipin, where higher dosage of genipin treatment resulted in the increased reactive oxygen species level and cytotoxicity. Thus, this study demonstrates for the first time that lower dosage of genipin results in the induction of JNK/Nrf2/ARE signaling pathway and protection from cell death.

Original languageEnglish
Pages (from-to)45-54
Number of pages10
JournalJournal of Biochemical and Molecular Toxicology
Volume30
Issue number1
DOIs
Publication statusPublished - 2016 Jan 1

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Keywords

  • AGS Cells
  • Genipin
  • JNK
  • Nrf2
  • Reactive Oxygen Species

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Molecular Medicine
  • Toxicology
  • Health, Toxicology and Mutagenesis

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