Chibby, a nuclear β-catenin-associated antagonist of the Wnt/Wingless pathway

Ken Ichi Takemaru, Shinji Yamaguchi, Young Sik Lee, Yang Zhang, Richard W. Carthew, Randall T. Moon

Research output: Contribution to journalArticlepeer-review

243 Citations (Scopus)


Inappropriate activation of downstream target genes by the oncoprotein β-catenin is implicated in development of numerous human cancers1,2. β-catenin and its fruitfly counterpart Armadillo act as a coactivator in the canonical Wnt/Wingless pathway by binding to Tcf/Lef transcription factors3-6. Here we report a conserved nuclear protein, named Chibby, which was identified in a screen for proteins that directly interact with the C-terminal region of β-catenin. In mammalian cultured cells we demonstrate that Chibby inhibits β-catenin-mediated transcriptional activation by competing with Lef-1 to bind to β-catenin. Inhibition of Drosophila Chibby by RNA interference results in segment polarity defects that mimick a wingless gain-of-function phenotype, and overexpression of the wingless target genes engrailed and Ultrabithorax. In addition, epistasis experiments indicate that chibby acts downstream of wingless and upstream of armadillo.

Original languageEnglish
Pages (from-to)905-909
Number of pages5
Issue number6934
Publication statusPublished - 2003 Apr 24
Externally publishedYes

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Chibby, a nuclear β-catenin-associated antagonist of the Wnt/Wingless pathway'. Together they form a unique fingerprint.

Cite this