Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells

B. R. Lee, K. H. Lee, E. Kang, S. H. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The successful encapsulation of human hepatocellular carcinoma (HepG2) cells would assist applications in tissue engineering. In this paper, we have developed a method to produce chitosan-alginate microfibers within a microfluidic chip. Also, by suspending cells in the mixing solution, we fabricated HepG2 cell-laden fibers. The encapsulated HepG2 cells in the chitosan-alginate fibers were more viable than cells encapsulated in pure alginate fibers, suggesting that chitosan provides a better environment for HepG2 cells than alginate. The proposed technique provided an uniform distribution of cells within the fibers, which has been a critical yet previously unmet requirement for tissue engineering.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1125-1127
Number of pages3
Publication statusPublished - 2011 Dec 1
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Keywords

  • Chitosan-alginate microfiber
  • Encapsulation
  • HepG2 cell
  • Microfluidic chip
  • Water-soluble chitosan

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Lee, B. R., Lee, K. H., Kang, E., & Lee, S. H. (2011). Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1125-1127). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).