Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells

B. R. Lee, K. H. Lee, E. Kang, Sang Hoon Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The successful encapsulation of human hepatocellular carcinoma (HepG2) cells would assist applications in tissue engineering. In this paper, we have developed a method to produce chitosan-alginate microfibers within a microfluidic chip. Also, by suspending cells in the mixing solution, we fabricated HepG2 cell-laden fibers. The encapsulated HepG2 cells in the chitosan-alginate fibers were more viable than cells encapsulated in pure alginate fibers, suggesting that chitosan provides a better environment for HepG2 cells than alginate. The proposed technique provided an uniform distribution of cells within the fibers, which has been a critical yet previously unmet requirement for tissue engineering.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1125-1127
Number of pages3
Volume2
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2011 Oct 22011 Oct 6

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period11/10/211/10/6

Fingerprint

Alginate
Chitosan
Encapsulation
Microfluidics
Fibers
Tissue engineering

Keywords

  • Chitosan-alginate microfiber
  • Encapsulation
  • HepG2 cell
  • Microfluidic chip
  • Water-soluble chitosan

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Lee, B. R., Lee, K. H., Kang, E., & Lee, S. H. (2011). Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 2, pp. 1125-1127)

Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells. / Lee, B. R.; Lee, K. H.; Kang, E.; Lee, Sang Hoon.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. p. 1125-1127.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lee, BR, Lee, KH, Kang, E & Lee, SH 2011, Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 2, pp. 1125-1127, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 11/10/2.
Lee BR, Lee KH, Kang E, Lee SH. Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2. 2011. p. 1125-1127
Lee, B. R. ; Lee, K. H. ; Kang, E. ; Lee, Sang Hoon. / Chitosan-alginate microfibers generation on a microfluidic chip and encapsulation of HepG2 cells. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. pp. 1125-1127
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