Chitosan functionalized thermosponge nano-carriers for prolonged retention and local delivery of chymopapain at the nucleus pulposus in porcine discs ex vivo

Won Il Choi, Abhishek Sahu, Cristian Vilos, Jong Hyun Lee, Sunghyun Kim, Young Ki Hong, Dong Geun Sul, Sun Wook Hwang, Sang Heon Lee, Giyoong Tae

Research output: Contribution to journalArticle

Abstract

Injection of chymopapain, a proteolytic enzyme, has been used in clinics for the treatment of spinal disc herniation, which is the major cause of lower back pain. However, the fast and wide diffusion and spreading of the enzyme from the injection site limit its effect and could cause severe side effects. Pluronic based nano-carriers were employed for the localized delivery of chymopapain and characterized in a porcine disc ex vivo as well as in vitro. Bare and chitosan conjugated thermosponge nano-carriers were prepared by photo-crosslinking either diacrylated Pluronic F127 or diacrylated Pluronic F68. Chymopapain was efficiently loaded into the nano-carriers with an encapsulation efficiency of over 95% in all the nano-carriers. In vitro release studies in physiological conditions (pH 7.4, 37 °C) showed a sustained release of the enzyme from all the nano-carriers but release from chitosan modified nano-carriers (∼16 days) was slower than that from the bare nano-carriers (∼12 days). Chymopapain delivered by nano-carriers remained more localized at the injection site in the nucleus pulposus of ex vivo porcine discs, compared to free chymopapain. Chitosan functionalized nano-carriers showed longer retention and slower diffusion of the enzyme compared to bare nano-carriers. The results suggest the potential use of the nano-carrier for the local delivery of chymopapain at the nucleus pulposus in discs.

Original languageEnglish
Pages (from-to)90967-90972
Number of pages6
JournalRSC Advances
Volume6
Issue number93
DOIs
Publication statusPublished - 2016

Fingerprint

Chymopapain
Chitosan
Enzymes
Poloxamer
UCON 50-HB-5100
Encapsulation
Crosslinking
Peptide Hydrolases

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Chitosan functionalized thermosponge nano-carriers for prolonged retention and local delivery of chymopapain at the nucleus pulposus in porcine discs ex vivo. / Choi, Won Il; Sahu, Abhishek; Vilos, Cristian; Lee, Jong Hyun; Kim, Sunghyun; Hong, Young Ki; Sul, Dong Geun; Hwang, Sun Wook; Lee, Sang Heon; Tae, Giyoong.

In: RSC Advances, Vol. 6, No. 93, 2016, p. 90967-90972.

Research output: Contribution to journalArticle

Choi, Won Il ; Sahu, Abhishek ; Vilos, Cristian ; Lee, Jong Hyun ; Kim, Sunghyun ; Hong, Young Ki ; Sul, Dong Geun ; Hwang, Sun Wook ; Lee, Sang Heon ; Tae, Giyoong. / Chitosan functionalized thermosponge nano-carriers for prolonged retention and local delivery of chymopapain at the nucleus pulposus in porcine discs ex vivo. In: RSC Advances. 2016 ; Vol. 6, No. 93. pp. 90967-90972.
@article{f7029cb6f29f47969dafb3a438f55ebe,
title = "Chitosan functionalized thermosponge nano-carriers for prolonged retention and local delivery of chymopapain at the nucleus pulposus in porcine discs ex vivo",
abstract = "Injection of chymopapain, a proteolytic enzyme, has been used in clinics for the treatment of spinal disc herniation, which is the major cause of lower back pain. However, the fast and wide diffusion and spreading of the enzyme from the injection site limit its effect and could cause severe side effects. Pluronic based nano-carriers were employed for the localized delivery of chymopapain and characterized in a porcine disc ex vivo as well as in vitro. Bare and chitosan conjugated thermosponge nano-carriers were prepared by photo-crosslinking either diacrylated Pluronic F127 or diacrylated Pluronic F68. Chymopapain was efficiently loaded into the nano-carriers with an encapsulation efficiency of over 95{\%} in all the nano-carriers. In vitro release studies in physiological conditions (pH 7.4, 37 °C) showed a sustained release of the enzyme from all the nano-carriers but release from chitosan modified nano-carriers (∼16 days) was slower than that from the bare nano-carriers (∼12 days). Chymopapain delivered by nano-carriers remained more localized at the injection site in the nucleus pulposus of ex vivo porcine discs, compared to free chymopapain. Chitosan functionalized nano-carriers showed longer retention and slower diffusion of the enzyme compared to bare nano-carriers. The results suggest the potential use of the nano-carrier for the local delivery of chymopapain at the nucleus pulposus in discs.",
author = "Choi, {Won Il} and Abhishek Sahu and Cristian Vilos and Lee, {Jong Hyun} and Sunghyun Kim and Hong, {Young Ki} and Sul, {Dong Geun} and Hwang, {Sun Wook} and Lee, {Sang Heon} and Giyoong Tae",
year = "2016",
doi = "10.1039/c6ra17848k",
language = "English",
volume = "6",
pages = "90967--90972",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "Royal Society of Chemistry",
number = "93",

}

TY - JOUR

T1 - Chitosan functionalized thermosponge nano-carriers for prolonged retention and local delivery of chymopapain at the nucleus pulposus in porcine discs ex vivo

AU - Choi, Won Il

AU - Sahu, Abhishek

AU - Vilos, Cristian

AU - Lee, Jong Hyun

AU - Kim, Sunghyun

AU - Hong, Young Ki

AU - Sul, Dong Geun

AU - Hwang, Sun Wook

AU - Lee, Sang Heon

AU - Tae, Giyoong

PY - 2016

Y1 - 2016

N2 - Injection of chymopapain, a proteolytic enzyme, has been used in clinics for the treatment of spinal disc herniation, which is the major cause of lower back pain. However, the fast and wide diffusion and spreading of the enzyme from the injection site limit its effect and could cause severe side effects. Pluronic based nano-carriers were employed for the localized delivery of chymopapain and characterized in a porcine disc ex vivo as well as in vitro. Bare and chitosan conjugated thermosponge nano-carriers were prepared by photo-crosslinking either diacrylated Pluronic F127 or diacrylated Pluronic F68. Chymopapain was efficiently loaded into the nano-carriers with an encapsulation efficiency of over 95% in all the nano-carriers. In vitro release studies in physiological conditions (pH 7.4, 37 °C) showed a sustained release of the enzyme from all the nano-carriers but release from chitosan modified nano-carriers (∼16 days) was slower than that from the bare nano-carriers (∼12 days). Chymopapain delivered by nano-carriers remained more localized at the injection site in the nucleus pulposus of ex vivo porcine discs, compared to free chymopapain. Chitosan functionalized nano-carriers showed longer retention and slower diffusion of the enzyme compared to bare nano-carriers. The results suggest the potential use of the nano-carrier for the local delivery of chymopapain at the nucleus pulposus in discs.

AB - Injection of chymopapain, a proteolytic enzyme, has been used in clinics for the treatment of spinal disc herniation, which is the major cause of lower back pain. However, the fast and wide diffusion and spreading of the enzyme from the injection site limit its effect and could cause severe side effects. Pluronic based nano-carriers were employed for the localized delivery of chymopapain and characterized in a porcine disc ex vivo as well as in vitro. Bare and chitosan conjugated thermosponge nano-carriers were prepared by photo-crosslinking either diacrylated Pluronic F127 or diacrylated Pluronic F68. Chymopapain was efficiently loaded into the nano-carriers with an encapsulation efficiency of over 95% in all the nano-carriers. In vitro release studies in physiological conditions (pH 7.4, 37 °C) showed a sustained release of the enzyme from all the nano-carriers but release from chitosan modified nano-carriers (∼16 days) was slower than that from the bare nano-carriers (∼12 days). Chymopapain delivered by nano-carriers remained more localized at the injection site in the nucleus pulposus of ex vivo porcine discs, compared to free chymopapain. Chitosan functionalized nano-carriers showed longer retention and slower diffusion of the enzyme compared to bare nano-carriers. The results suggest the potential use of the nano-carrier for the local delivery of chymopapain at the nucleus pulposus in discs.

UR - http://www.scopus.com/inward/record.url?scp=84989285874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84989285874&partnerID=8YFLogxK

U2 - 10.1039/c6ra17848k

DO - 10.1039/c6ra17848k

M3 - Article

AN - SCOPUS:84989285874

VL - 6

SP - 90967

EP - 90972

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 93

ER -