Chitosan nanoparticles show rapid extrapulmonary tissue distribution and excretion with mild pulmonary inflammation to micen

Mina Choi, Minjung Cho, Beom Seok Han, Jin Hong, Jayoung Jeong, Sangjin Park, Myung Haing Cho, Kwangmeyung Kim, Wan Seob Cho

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Pulmonary delivery of nanoparticles (NP) conjugated with therapeutic agents has been considered recently for both lung disorders and systemic circulation. Hydrophobically modified glycol chitosan (HGC) NP have previously shown excellent deposition to the tumor site and non-destructive intracellular release. Here, we evaluated the kinetics and toxicity of HGC NP by intratracheal instillation to mice. HGC NP showed a positive charge and average hydrodynamic size was around 350. nm. The half-life of NP in the lung was determined as 131.97 ± 50.51. h. NP showed rapid uptake into systemic circulation and excretion via urine which was peaked at 6. h after instillation. Although HGC NP were distributed to several extrapulmonary organs, the levels were extremely low and transient. HGC NP induced transient neutrophilic pulmonary inflammation from 6. h to day 3 after instillation. Expression of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and chemokine (MIP-1α) in lung showed an increase from 1. h to 24. h after instillation and recovered thereafter. Our findings suggest that HGC NP can be successful candidates for use as pulmonary delivery vehicles, owing to their excellent biocompatibility, transiency, and low pulmonary toxicity, and property of rapid elimination without accumulation.

Original languageEnglish
Pages (from-to)144-152
Number of pages9
JournalToxicology Letters
Volume199
Issue number2
DOIs
Publication statusPublished - 2010 Nov 30
Externally publishedYes

Keywords

  • Elimination
  • Hydrophobically modified glycol chitosan
  • Intratracheal instillation
  • Pulmonary delivery vehicle
  • Tissue distribution

ASJC Scopus subject areas

  • Toxicology

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