Chlorine dioxide enhances lipid peroxidation through inhibiting calcium-independent cellular PLA2 in larvae of the Indianmeal moth, Plodia interpunctella

Gyung Deok Han, Jahyun Na, Yong Shik Chun, Sunil Kumar, Wook Kim, Yonggyun Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Polyunsaturated fatty acids usually undergo lipid peroxidation induced by reactive oxygen species (ROS). Calcium-independent cellular phospholipase A2 (iPLA2) can maintain fatty acid compositions in phospholipids depending on physiological conditions. An insect iPLA2 (Pi-iPLA2) was predicted from the transciptome of the Indianmeal moth, Plodia interpunctella. It encodes 835 amino acids. It possesses five ankyrin repeats in the N terminal and patatin lipase domain in the C terminal. Pi-iPLA 2 was expressed in all developmental stages of the Indianmeal moth. In the larval stage, it was expressed in all tissues tested. RNA interference (RNAi) specific to Pi-iPLA 2 was performed using specific double-stranded RNA (dsRNA). It resulted in almost 70% of reduction in gene expression. Under such RNAi condition, P. interpunctella exhibited significant accumulation of lipid peroxidation based on the amount of malondialdehyde. RNAi of Pi-PLA 2 expression also impaired cellular immune response of P. interpunctella. Chlorine dioxide (ClO2), an insecticidal agent by generating ROS, increased lipid peroxidation in a dose-dependent manner. However, the addition of vitamin E (an antioxidant) reduced the formation of lipid peroxidation. ClO2 treatment significantly reduced expression of Pi-iPLA 2 but increased lipid peroxidation in larval fat body of P. interpunctella. Furthermore, larvae treated with dsRNA specific to Pi-iPLA 2 were significantly susceptible to ClO2 treatment. These results suggest that Pi-iPLA2 plays a crucial role in repairing damaged fatty acids from phospholipids. Our results also suggest that ClO2 can elevate lipid peroxidation through inhibiting Pi-iPLA 2 expression in addition to direct ROS production.

Original languageEnglish
JournalPesticide Biochemistry and Physiology
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Calcium-Independent Phospholipase A2
chlorine dioxide
Plodia interpunctella
Moths
Lipid Peroxidation
Larva
lipid peroxidation
phospholipase A2
Calcium
calcium
larvae
Phospholipases A2
RNA Interference
RNA interference
reactive oxygen species
Reactive Oxygen Species
Double-Stranded RNA
double-stranded RNA
Phospholipids
phospholipids

Keywords

  • Chlorine dioxide
  • Immunity
  • Lipid peroxidation
  • PLA
  • Plodia interpunctella

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

Cite this

Chlorine dioxide enhances lipid peroxidation through inhibiting calcium-independent cellular PLA2 in larvae of the Indianmeal moth, Plodia interpunctella. / Han, Gyung Deok; Na, Jahyun; Chun, Yong Shik; Kumar, Sunil; Kim, Wook; Kim, Yonggyun.

In: Pesticide Biochemistry and Physiology, 2017.

Research output: Contribution to journalArticle

@article{b37e84a14aa24c489f0954004d130a4b,
title = "Chlorine dioxide enhances lipid peroxidation through inhibiting calcium-independent cellular PLA2 in larvae of the Indianmeal moth, Plodia interpunctella",
abstract = "Polyunsaturated fatty acids usually undergo lipid peroxidation induced by reactive oxygen species (ROS). Calcium-independent cellular phospholipase A2 (iPLA2) can maintain fatty acid compositions in phospholipids depending on physiological conditions. An insect iPLA2 (Pi-iPLA2) was predicted from the transciptome of the Indianmeal moth, Plodia interpunctella. It encodes 835 amino acids. It possesses five ankyrin repeats in the N terminal and patatin lipase domain in the C terminal. Pi-iPLA 2 was expressed in all developmental stages of the Indianmeal moth. In the larval stage, it was expressed in all tissues tested. RNA interference (RNAi) specific to Pi-iPLA 2 was performed using specific double-stranded RNA (dsRNA). It resulted in almost 70{\%} of reduction in gene expression. Under such RNAi condition, P. interpunctella exhibited significant accumulation of lipid peroxidation based on the amount of malondialdehyde. RNAi of Pi-PLA 2 expression also impaired cellular immune response of P. interpunctella. Chlorine dioxide (ClO2), an insecticidal agent by generating ROS, increased lipid peroxidation in a dose-dependent manner. However, the addition of vitamin E (an antioxidant) reduced the formation of lipid peroxidation. ClO2 treatment significantly reduced expression of Pi-iPLA 2 but increased lipid peroxidation in larval fat body of P. interpunctella. Furthermore, larvae treated with dsRNA specific to Pi-iPLA 2 were significantly susceptible to ClO2 treatment. These results suggest that Pi-iPLA2 plays a crucial role in repairing damaged fatty acids from phospholipids. Our results also suggest that ClO2 can elevate lipid peroxidation through inhibiting Pi-iPLA 2 expression in addition to direct ROS production.",
keywords = "Chlorine dioxide, Immunity, Lipid peroxidation, PLA, Plodia interpunctella",
author = "Han, {Gyung Deok} and Jahyun Na and Chun, {Yong Shik} and Sunil Kumar and Wook Kim and Yonggyun Kim",
year = "2017",
doi = "10.1016/j.pestbp.2017.09.010",
language = "English",
journal = "Pesticide Biochemistry and Physiology",
issn = "0048-3575",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Chlorine dioxide enhances lipid peroxidation through inhibiting calcium-independent cellular PLA2 in larvae of the Indianmeal moth, Plodia interpunctella

AU - Han, Gyung Deok

AU - Na, Jahyun

AU - Chun, Yong Shik

AU - Kumar, Sunil

AU - Kim, Wook

AU - Kim, Yonggyun

PY - 2017

Y1 - 2017

N2 - Polyunsaturated fatty acids usually undergo lipid peroxidation induced by reactive oxygen species (ROS). Calcium-independent cellular phospholipase A2 (iPLA2) can maintain fatty acid compositions in phospholipids depending on physiological conditions. An insect iPLA2 (Pi-iPLA2) was predicted from the transciptome of the Indianmeal moth, Plodia interpunctella. It encodes 835 amino acids. It possesses five ankyrin repeats in the N terminal and patatin lipase domain in the C terminal. Pi-iPLA 2 was expressed in all developmental stages of the Indianmeal moth. In the larval stage, it was expressed in all tissues tested. RNA interference (RNAi) specific to Pi-iPLA 2 was performed using specific double-stranded RNA (dsRNA). It resulted in almost 70% of reduction in gene expression. Under such RNAi condition, P. interpunctella exhibited significant accumulation of lipid peroxidation based on the amount of malondialdehyde. RNAi of Pi-PLA 2 expression also impaired cellular immune response of P. interpunctella. Chlorine dioxide (ClO2), an insecticidal agent by generating ROS, increased lipid peroxidation in a dose-dependent manner. However, the addition of vitamin E (an antioxidant) reduced the formation of lipid peroxidation. ClO2 treatment significantly reduced expression of Pi-iPLA 2 but increased lipid peroxidation in larval fat body of P. interpunctella. Furthermore, larvae treated with dsRNA specific to Pi-iPLA 2 were significantly susceptible to ClO2 treatment. These results suggest that Pi-iPLA2 plays a crucial role in repairing damaged fatty acids from phospholipids. Our results also suggest that ClO2 can elevate lipid peroxidation through inhibiting Pi-iPLA 2 expression in addition to direct ROS production.

AB - Polyunsaturated fatty acids usually undergo lipid peroxidation induced by reactive oxygen species (ROS). Calcium-independent cellular phospholipase A2 (iPLA2) can maintain fatty acid compositions in phospholipids depending on physiological conditions. An insect iPLA2 (Pi-iPLA2) was predicted from the transciptome of the Indianmeal moth, Plodia interpunctella. It encodes 835 amino acids. It possesses five ankyrin repeats in the N terminal and patatin lipase domain in the C terminal. Pi-iPLA 2 was expressed in all developmental stages of the Indianmeal moth. In the larval stage, it was expressed in all tissues tested. RNA interference (RNAi) specific to Pi-iPLA 2 was performed using specific double-stranded RNA (dsRNA). It resulted in almost 70% of reduction in gene expression. Under such RNAi condition, P. interpunctella exhibited significant accumulation of lipid peroxidation based on the amount of malondialdehyde. RNAi of Pi-PLA 2 expression also impaired cellular immune response of P. interpunctella. Chlorine dioxide (ClO2), an insecticidal agent by generating ROS, increased lipid peroxidation in a dose-dependent manner. However, the addition of vitamin E (an antioxidant) reduced the formation of lipid peroxidation. ClO2 treatment significantly reduced expression of Pi-iPLA 2 but increased lipid peroxidation in larval fat body of P. interpunctella. Furthermore, larvae treated with dsRNA specific to Pi-iPLA 2 were significantly susceptible to ClO2 treatment. These results suggest that Pi-iPLA2 plays a crucial role in repairing damaged fatty acids from phospholipids. Our results also suggest that ClO2 can elevate lipid peroxidation through inhibiting Pi-iPLA 2 expression in addition to direct ROS production.

KW - Chlorine dioxide

KW - Immunity

KW - Lipid peroxidation

KW - PLA

KW - Plodia interpunctella

UR - http://www.scopus.com/inward/record.url?scp=85029769606&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029769606&partnerID=8YFLogxK

U2 - 10.1016/j.pestbp.2017.09.010

DO - 10.1016/j.pestbp.2017.09.010

M3 - Article

JO - Pesticide Biochemistry and Physiology

JF - Pesticide Biochemistry and Physiology

SN - 0048-3575

ER -