Abstract
Volatile organic compounds (VOCs) are major air pollutants known to have adverse effects on skin. Many reports have shown an association between air pollutants and skin aging. However, there is no report on VOCs association with skin aging. The aim of this study is to identify the biologic effects of VOCs on fibroblasts. We investigated the effects of chloroform on cysteine-rich 61 (CYR61) expression in human skin fibroblasts using western blot and reverse transcriptionpolymerase chain reaction. We also observed the change of type I procollagen and matrix metalloproteinase after exposure to chloroform using western blot. We explored the involvement of early growth response-1 (Egr-1) using siRNA s in the fibroblasts. Chloroform exposure increased the expression of CYR61 protein and mRNA level. Elevated CYR61 downregulates type I procollagen and upregulates MMP-1. Egr-1 overexpression upregulates CYR61 promoter activity. CYR61 promoter activity was lower in Egr-1 siRNA-transfected fibroblasts. The present study demonstrates that CYR61 is transcriptionally regulated by chloroform exposure through transcription factor Egr-1, and alters collagen homeostasis in human skin fibroblasts.
| Original language | English |
|---|---|
| Pages (from-to) | 337-343 |
| Number of pages | 7 |
| Journal | Molecular and Cellular Toxicology |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2016 Dec 1 |
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Keywords
- Chloroform
- Cysteine-rich 61
- Early growth response-1
- Skin fibroblast
- Volatile organic compound
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Toxicology
- Pharmacology, Toxicology and Pharmaceutics(all)
- Public Health, Environmental and Occupational Health
- Health, Toxicology and Mutagenesis
Cite this
Chloroform induces cystein-rich 61, a mediator of collagen homeostasis via early growth response-1 dependent pathway in human skin dermal fibroblasts. / Lee, Hana; Park, Jae Beom; Ryu, Woo In; Kim, Ji Hyun; Shin, Jung Jin; Son, Sang Wook.
In: Molecular and Cellular Toxicology, Vol. 12, No. 4, 01.12.2016, p. 337-343.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chloroform induces cystein-rich 61, a mediator of collagen homeostasis via early growth response-1 dependent pathway in human skin dermal fibroblasts
AU - Lee, Hana
AU - Park, Jae Beom
AU - Ryu, Woo In
AU - Kim, Ji Hyun
AU - Shin, Jung Jin
AU - Son, Sang Wook
PY - 2016/12/1
Y1 - 2016/12/1
N2 - Volatile organic compounds (VOCs) are major air pollutants known to have adverse effects on skin. Many reports have shown an association between air pollutants and skin aging. However, there is no report on VOCs association with skin aging. The aim of this study is to identify the biologic effects of VOCs on fibroblasts. We investigated the effects of chloroform on cysteine-rich 61 (CYR61) expression in human skin fibroblasts using western blot and reverse transcriptionpolymerase chain reaction. We also observed the change of type I procollagen and matrix metalloproteinase after exposure to chloroform using western blot. We explored the involvement of early growth response-1 (Egr-1) using siRNA s in the fibroblasts. Chloroform exposure increased the expression of CYR61 protein and mRNA level. Elevated CYR61 downregulates type I procollagen and upregulates MMP-1. Egr-1 overexpression upregulates CYR61 promoter activity. CYR61 promoter activity was lower in Egr-1 siRNA-transfected fibroblasts. The present study demonstrates that CYR61 is transcriptionally regulated by chloroform exposure through transcription factor Egr-1, and alters collagen homeostasis in human skin fibroblasts.
AB - Volatile organic compounds (VOCs) are major air pollutants known to have adverse effects on skin. Many reports have shown an association between air pollutants and skin aging. However, there is no report on VOCs association with skin aging. The aim of this study is to identify the biologic effects of VOCs on fibroblasts. We investigated the effects of chloroform on cysteine-rich 61 (CYR61) expression in human skin fibroblasts using western blot and reverse transcriptionpolymerase chain reaction. We also observed the change of type I procollagen and matrix metalloproteinase after exposure to chloroform using western blot. We explored the involvement of early growth response-1 (Egr-1) using siRNA s in the fibroblasts. Chloroform exposure increased the expression of CYR61 protein and mRNA level. Elevated CYR61 downregulates type I procollagen and upregulates MMP-1. Egr-1 overexpression upregulates CYR61 promoter activity. CYR61 promoter activity was lower in Egr-1 siRNA-transfected fibroblasts. The present study demonstrates that CYR61 is transcriptionally regulated by chloroform exposure through transcription factor Egr-1, and alters collagen homeostasis in human skin fibroblasts.
KW - Chloroform
KW - Cysteine-rich 61
KW - Early growth response-1
KW - Skin fibroblast
KW - Volatile organic compound
UR - http://www.scopus.com/inward/record.url?scp=85008449925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85008449925&partnerID=8YFLogxK
U2 - 10.1007/s13273-016-0038-6
DO - 10.1007/s13273-016-0038-6
M3 - Article
AN - SCOPUS:85008449925
VL - 12
SP - 337
EP - 343
JO - Molecular and Cellular Toxicology
JF - Molecular and Cellular Toxicology
SN - 1738-642X
IS - 4
ER -