Chloroform upregulates early growth response-1-dependent thymic stromal lymphopoietin expression via the JNK and ERK pathways in human keratinocytes

Hana Lee, Hyun Cheol Bae, Jinhee Kim, Sang Hoon Jeong, Woo In Ryu, Sang Wook Son

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Exposure to volatile organic compounds (VOCs) in the environment has been reported to exacerbate allergic inflammatory diseases, such as atopic dermatitis (AD). However, the exact mechanism by which VOCs induce an inflammatory response in the skin is poorly understood. Thymic stromal lymphopoietin (TSLP) is known to be an important factor in the initiation and maintenance of allergic inflammatory diseases, including AD. Objectives: The aim of this work is to define the correlation between VOCs and TSLP. Methods: The present study demonstrates dose-dependent increases of TSLP protein and mRNA levels in keratinocytes following exposure to chloroform. We further investigated the regulatory mechanisms of chloroform-induced TSLP expression in human keratinocytes. Results: Chloroform induces early growth response-1 (Egr-1) protein expression in human keratinocytes. This process is mediated by the c-JUN N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) signaling pathways. Inhibition of phosphorylated JNK and ERK significantly downregulated Egr-1 expression, which was subsequently associated with reduced TSLP expression in chloroform-exposed human keratinocytes. Moreover, treatment of Egr-1 siRNA abolished chloroform-induced TSLP protein expression and TSLP promoter transcriptional activation. Conclusions: Taken together, these findings suggest that, in human keratinocytes, the upregulation of TSLP by chloroform is induced through an Egr-1-dependent mechanism that requires the c-JNK and ERK pathways. Our results suggest that exposure to chloroform may aggravate allergic skin diseases such as AD through Egr-1-dependent TSLP regulation.

Original languageEnglish
Pages (from-to)e521-e526
JournalInternational Journal of Dermatology
Volume54
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

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Extracellular Signal-Regulated MAP Kinases
Chloroform
Keratinocytes
Phosphotransferases
Up-Regulation
Growth
Volatile Organic Compounds
Atopic Dermatitis
Early Growth Response Protein 1
thymic stromal lymphopoietin
Peptide Initiation Factors
Skin Diseases
Small Interfering RNA
Transcriptional Activation
Proteins
Down-Regulation
Maintenance
Messenger RNA
Skin

ASJC Scopus subject areas

  • Dermatology

Cite this

Chloroform upregulates early growth response-1-dependent thymic stromal lymphopoietin expression via the JNK and ERK pathways in human keratinocytes. / Lee, Hana; Bae, Hyun Cheol; Kim, Jinhee; Jeong, Sang Hoon; Ryu, Woo In; Son, Sang Wook.

In: International Journal of Dermatology, Vol. 54, No. 12, 01.12.2015, p. e521-e526.

Research output: Contribution to journalArticle

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abstract = "Background: Exposure to volatile organic compounds (VOCs) in the environment has been reported to exacerbate allergic inflammatory diseases, such as atopic dermatitis (AD). However, the exact mechanism by which VOCs induce an inflammatory response in the skin is poorly understood. Thymic stromal lymphopoietin (TSLP) is known to be an important factor in the initiation and maintenance of allergic inflammatory diseases, including AD. Objectives: The aim of this work is to define the correlation between VOCs and TSLP. Methods: The present study demonstrates dose-dependent increases of TSLP protein and mRNA levels in keratinocytes following exposure to chloroform. We further investigated the regulatory mechanisms of chloroform-induced TSLP expression in human keratinocytes. Results: Chloroform induces early growth response-1 (Egr-1) protein expression in human keratinocytes. This process is mediated by the c-JUN N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) signaling pathways. Inhibition of phosphorylated JNK and ERK significantly downregulated Egr-1 expression, which was subsequently associated with reduced TSLP expression in chloroform-exposed human keratinocytes. Moreover, treatment of Egr-1 siRNA abolished chloroform-induced TSLP protein expression and TSLP promoter transcriptional activation. Conclusions: Taken together, these findings suggest that, in human keratinocytes, the upregulation of TSLP by chloroform is induced through an Egr-1-dependent mechanism that requires the c-JNK and ERK pathways. Our results suggest that exposure to chloroform may aggravate allergic skin diseases such as AD through Egr-1-dependent TSLP regulation.",
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AU - Bae, Hyun Cheol

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AU - Ryu, Woo In

AU - Son, Sang Wook

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N2 - Background: Exposure to volatile organic compounds (VOCs) in the environment has been reported to exacerbate allergic inflammatory diseases, such as atopic dermatitis (AD). However, the exact mechanism by which VOCs induce an inflammatory response in the skin is poorly understood. Thymic stromal lymphopoietin (TSLP) is known to be an important factor in the initiation and maintenance of allergic inflammatory diseases, including AD. Objectives: The aim of this work is to define the correlation between VOCs and TSLP. Methods: The present study demonstrates dose-dependent increases of TSLP protein and mRNA levels in keratinocytes following exposure to chloroform. We further investigated the regulatory mechanisms of chloroform-induced TSLP expression in human keratinocytes. Results: Chloroform induces early growth response-1 (Egr-1) protein expression in human keratinocytes. This process is mediated by the c-JUN N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) signaling pathways. Inhibition of phosphorylated JNK and ERK significantly downregulated Egr-1 expression, which was subsequently associated with reduced TSLP expression in chloroform-exposed human keratinocytes. Moreover, treatment of Egr-1 siRNA abolished chloroform-induced TSLP protein expression and TSLP promoter transcriptional activation. Conclusions: Taken together, these findings suggest that, in human keratinocytes, the upregulation of TSLP by chloroform is induced through an Egr-1-dependent mechanism that requires the c-JNK and ERK pathways. Our results suggest that exposure to chloroform may aggravate allergic skin diseases such as AD through Egr-1-dependent TSLP regulation.

AB - Background: Exposure to volatile organic compounds (VOCs) in the environment has been reported to exacerbate allergic inflammatory diseases, such as atopic dermatitis (AD). However, the exact mechanism by which VOCs induce an inflammatory response in the skin is poorly understood. Thymic stromal lymphopoietin (TSLP) is known to be an important factor in the initiation and maintenance of allergic inflammatory diseases, including AD. Objectives: The aim of this work is to define the correlation between VOCs and TSLP. Methods: The present study demonstrates dose-dependent increases of TSLP protein and mRNA levels in keratinocytes following exposure to chloroform. We further investigated the regulatory mechanisms of chloroform-induced TSLP expression in human keratinocytes. Results: Chloroform induces early growth response-1 (Egr-1) protein expression in human keratinocytes. This process is mediated by the c-JUN N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK) signaling pathways. Inhibition of phosphorylated JNK and ERK significantly downregulated Egr-1 expression, which was subsequently associated with reduced TSLP expression in chloroform-exposed human keratinocytes. Moreover, treatment of Egr-1 siRNA abolished chloroform-induced TSLP protein expression and TSLP promoter transcriptional activation. Conclusions: Taken together, these findings suggest that, in human keratinocytes, the upregulation of TSLP by chloroform is induced through an Egr-1-dependent mechanism that requires the c-JNK and ERK pathways. Our results suggest that exposure to chloroform may aggravate allergic skin diseases such as AD through Egr-1-dependent TSLP regulation.

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