Chondrogenic differentiation of human mesenchymal stem cells on oriented nanofibrous scaffolds: Engineering the superficial zone of articular cartilage

Joel K. Wise, Alexander Yarin, Constantine M. Megaridis, Michael Cho

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Cell differentiation, adhesion, and orientation are known to influence the functionality of both natural and engineered tissues, such as articular cartilage. Several attempts have been devised to regulate these important cellular behaviors, including application of inexpensive but efficient electrospinning that can produce patterned extracellular matrix (ECM) features. Electrospun and oriented polycaprolactone (PCL) scaffolds (500 or 3000 nm fiber diameter) were created, and human mesenchymal stem cells (hMSCs) were cultured on these scaffolds. Cell viability, morphology, and orientation on the fibrous scaffolds were quantitatively determined as a function of time. While the fiber-guided initial cell orientation was maintained even after 5 weeks, cells cultured in the chondrogenic media proliferated and differentiated into the chondrogenic lineage, suggesting that cell orientation is controlled by the physical cues and minimally influenced by the soluble factors. Based on assessment by the chondrogenic markers, use of the nanofibrous scaffold (500 nm) appears to enhance the chondrogenic differentiation. These findings indicate that hMSCs seeded on a controllable PCL scaffold may lead to an alternate methodology to mimic the cell and ECM organization that is found, for example, in the superficial zone of articular cartilage.

Original languageEnglish
Pages (from-to)913-921
Number of pages9
JournalTissue Engineering - Part A
Volume15
Issue number4
DOIs
Publication statusPublished - 2009 Apr 1
Externally publishedYes

Fingerprint

Cartilage
Articular Cartilage
Stem cells
Mesenchymal Stromal Cells
Scaffolds
Extracellular Matrix
Polycaprolactone
Cell Adhesion
Cues
Cell Differentiation
Cultured Cells
Cell Survival
Fibers
Electrospinning
Adhesion
Cells
Tissue
polycaprolactone

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials

Cite this

Chondrogenic differentiation of human mesenchymal stem cells on oriented nanofibrous scaffolds : Engineering the superficial zone of articular cartilage. / Wise, Joel K.; Yarin, Alexander; Megaridis, Constantine M.; Cho, Michael.

In: Tissue Engineering - Part A, Vol. 15, No. 4, 01.04.2009, p. 913-921.

Research output: Contribution to journalArticle

Wise, Joel K. ; Yarin, Alexander ; Megaridis, Constantine M. ; Cho, Michael. / Chondrogenic differentiation of human mesenchymal stem cells on oriented nanofibrous scaffolds : Engineering the superficial zone of articular cartilage. In: Tissue Engineering - Part A. 2009 ; Vol. 15, No. 4. pp. 913-921.
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