Chromosomal aberrations in neuroblastoma cell lines identified by cross species color banding and chromosome painting

Gi Jin Kim, Soo Yeun Park, Hyun Kim, Yong Hyuck Chun, Sun-Hwa Park

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We have studied cytogenetic rearrangements in karyotypes of five neuroblastoma cell lines [SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, SMS-KCNR] by G-banding, cross species color banding (RxFISH), and fluorescence in situ hybridization (FISH) with chromosome painting probes. Each neuroblastoma cell line had unique modal karyotypic characteristics and showed a variable number of numerical and structural clonal cytogenetic aberrations. The number of rearranged chromosomes in SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, and SMS-KCNR was 11, 3, 7, 14 (tetraploid, 20-21), and 6, respectively. The origins of abnormal chromosomes were effectively analyzed by RxFISH and FISH with multiple chromosome painting probes. The chromosomal origin of the homogeneously staining region in SH-SY5Y was identified as coamplification of chromosome bands 2p13 and 2p24 by chromosome microdissection and FISH. The non-random rearrangements of chromosomes were determined on 1p34∼p36, 6q16∼q21, 8q24, 9q34, 11q13∼q23, 16q23∼q24, 17q21, and 22q31. These results may provide useful information for further molecular characterization of neuroblastoma.

Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalCancer Genetics and Cytogenetics
Issue number1
Publication statusPublished - 2001 Aug 23


ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

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