Chromosomal aberrations in neuroblastoma cell lines identified by cross species color banding and chromosome painting

Gi Jin Kim, Soo Yeun Park, Hyun Kim, Yong Hyuck Chun, Sun Hwa Park

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have studied cytogenetic rearrangements in karyotypes of five neuroblastoma cell lines [SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, SMS-KCNR] by G-banding, cross species color banding (RxFISH), and fluorescence in situ hybridization (FISH) with chromosome painting probes. Each neuroblastoma cell line had unique modal karyotypic characteristics and showed a variable number of numerical and structural clonal cytogenetic aberrations. The number of rearranged chromosomes in SK-N-AS, SK-N-SH, SH-SY5Y, SK-N-MC, and SMS-KCNR was 11, 3, 7, 14 (tetraploid, 20-21), and 6, respectively. The origins of abnormal chromosomes were effectively analyzed by RxFISH and FISH with multiple chromosome painting probes. The chromosomal origin of the homogeneously staining region in SH-SY5Y was identified as coamplification of chromosome bands 2p13 and 2p24 by chromosome microdissection and FISH. The non-random rearrangements of chromosomes were determined on 1p34∼p36, 6q16∼q21, 8q24, 9q34, 11q13∼q23, 16q23∼q24, 17q21, and 22q31. These results may provide useful information for further molecular characterization of neuroblastoma.

Original languageEnglish
Pages (from-to)10-16
Number of pages7
JournalCancer Genetics and Cytogenetics
Volume129
Issue number1
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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