Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase

Kyoung Ho Roh, Wonkeun Song, Hae Sun Chung, Yang Soon Lee, Jong Hwa Yum, Hana Yi, Jong Sik Chun, Dongeun Yong, Kyungwon Lee, Yunsop Chong

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Abstract

In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.

Original languageEnglish
Pages (from-to)94-100
Number of pages7
JournalJournal of Medical Microbiology
Volume61
Issue number1
DOIs
Publication statusPublished - 2012 Jan
Externally publishedYes

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ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Roh, K. H., Song, W., Chung, H. S., Lee, Y. S., Yum, J. H., Yi, H., Chun, J. S., Yong, D., Lee, K., & Chong, Y. (2012). Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase. Journal of Medical Microbiology, 61(1), 94-100. https://doi.org/10.1099/jmm.0.032573-0