Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase

Kyoung Ho Roh, Wonkeun Song, Hae Sun Chung, Yang Soon Lee, Jong Hwa Yum, Hana Yi, Jong Sik Chun, Dongeun Yong, Kyungwon Lee, Yunsop Chong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.

Original languageEnglish
Pages (from-to)94-100
Number of pages7
JournalJournal of Medical Microbiology
Volume61
Issue number1
DOIs
Publication statusPublished - 2012 Jan
Externally publishedYes

Fingerprint

Cephalosporinase
Enterobacter
Genes
Chromosomes, Human, 16-18
Plasmids
Cefoxitin
DNA
rRNA Genes
Aspartic Acid
Glycine
Serine
Tyrosine
Arginine
Real-Time Polymerase Chain Reaction
Amino Acid Sequence
Chromosomes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase. / Roh, Kyoung Ho; Song, Wonkeun; Chung, Hae Sun; Lee, Yang Soon; Yum, Jong Hwa; Yi, Hana; Chun, Jong Sik; Yong, Dongeun; Lee, Kyungwon; Chong, Yunsop.

In: Journal of Medical Microbiology, Vol. 61, No. 1, 01.2012, p. 94-100.

Research output: Contribution to journalArticle

Roh, Kyoung Ho ; Song, Wonkeun ; Chung, Hae Sun ; Lee, Yang Soon ; Yum, Jong Hwa ; Yi, Hana ; Chun, Jong Sik ; Yong, Dongeun ; Lee, Kyungwon ; Chong, Yunsop. / Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase. In: Journal of Medical Microbiology. 2012 ; Vol. 61, No. 1. pp. 94-100.
@article{9f707e619a664341967ed8dfed1dc1d3,
title = "Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase",
abstract = "In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.",
author = "Roh, {Kyoung Ho} and Wonkeun Song and Chung, {Hae Sun} and Lee, {Yang Soon} and Yum, {Jong Hwa} and Hana Yi and Chun, {Jong Sik} and Dongeun Yong and Kyungwon Lee and Yunsop Chong",
year = "2012",
month = "1",
doi = "10.1099/jmm.0.032573-0",
language = "English",
volume = "61",
pages = "94--100",
journal = "Journal of Medical Microbiology",
issn = "0022-2615",
publisher = "Society for General Microbiology",
number = "1",

}

TY - JOUR

T1 - Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmidmediated AmpC β-lactamase

AU - Roh, Kyoung Ho

AU - Song, Wonkeun

AU - Chung, Hae Sun

AU - Lee, Yang Soon

AU - Yum, Jong Hwa

AU - Yi, Hana

AU - Chun, Jong Sik

AU - Yong, Dongeun

AU - Lee, Kyungwon

AU - Chong, Yunsop

PY - 2012/1

Y1 - 2012/1

N2 - In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.

AB - In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the blaACT-1 gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the blaACT-1 gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated blaACT-1 gene originated from the chromosome of E. hormaechei.

UR - http://www.scopus.com/inward/record.url?scp=83755183392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=83755183392&partnerID=8YFLogxK

U2 - 10.1099/jmm.0.032573-0

DO - 10.1099/jmm.0.032573-0

M3 - Article

C2 - 21873382

AN - SCOPUS:83755183392

VL - 61

SP - 94

EP - 100

JO - Journal of Medical Microbiology

JF - Journal of Medical Microbiology

SN - 0022-2615

IS - 1

ER -