Chronic mild stress decreases survival, but not proliferation, of new-born cells in adult rat hippocampus

Kuem Ju Lee, Sung Jin Kim, Suk Won Kim, Song Hyen Choi, You Chan Shin, Sang Ha Park, Bo Hyun Moon, Eujin Cho, Min-Soo Lee, Sang-Hyun Choi, Boe Gwun Chun, Kyung-Ho Shin

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

New-born cells continue to proliferate and survive to become mature granule cells in adult rat hippocampus. Although this process, known as neurogenesis, is inhibited by acute stress, it is not clear whether chronic stress affects neurogenesis. To determine whether chronic mild stress (CMS) influences neurogenesis in the adult rat hippocampus, male Sprague-Dawley rats were exposed to CMS and administered bromodeoxyuridine (BrdU) before or after CMS to observe the survival/differentiation or proliferation of new-born cells, respectively. In addition, we measured brain-derived neurotrophic factor (BDNF) mRNA in the granule cell layer (GCL) of the hippocampus, because BDNF is known to play an important role in the survival of new-born cells. CMS significantly decreased the survival of newborn cells in the GCL, but did not influence the proliferation or differentiation of new-born cells. CMS did not affect the proliferation and survival of new-born cells in the hilus. In addition, CMS did not change BDNF mRNA levels in the GCL. These results demonstrate that CMS reduces the survival of new-born cells but not of their proliferation, suggesting that repeated mild stress could influence a part of neurogenesis, but not the whole part of neurogenesis. These results raise the possibility that the survival of new-born cells may be suppressed in the presence of normal BDNF mRNA levels in GCL.

Original languageEnglish
Pages (from-to)44-54
Number of pages11
JournalExperimental and Molecular Medicine
Volume38
Issue number1
DOIs
Publication statusPublished - 2006 Feb 28

Fingerprint

Rats
Hippocampus
Cells
Brain-Derived Neurotrophic Factor
Neurogenesis
Messenger RNA
Bromodeoxyuridine
Sprague Dawley Rats
Cell Survival

Keywords

  • Brain-derived neurotrophic factor
  • Bromodeoxyuridine
  • Depression
  • Hippocampus
  • Neurogenesis
  • Stress

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

Cite this

Chronic mild stress decreases survival, but not proliferation, of new-born cells in adult rat hippocampus. / Lee, Kuem Ju; Kim, Sung Jin; Kim, Suk Won; Choi, Song Hyen; Shin, You Chan; Park, Sang Ha; Moon, Bo Hyun; Cho, Eujin; Lee, Min-Soo; Choi, Sang-Hyun; Chun, Boe Gwun; Shin, Kyung-Ho.

In: Experimental and Molecular Medicine, Vol. 38, No. 1, 28.02.2006, p. 44-54.

Research output: Contribution to journalArticle

Lee, KJ, Kim, SJ, Kim, SW, Choi, SH, Shin, YC, Park, SH, Moon, BH, Cho, E, Lee, M-S, Choi, S-H, Chun, BG & Shin, K-H 2006, 'Chronic mild stress decreases survival, but not proliferation, of new-born cells in adult rat hippocampus', Experimental and Molecular Medicine, vol. 38, no. 1, pp. 44-54. https://doi.org/10.1038/emm.2006.6
Lee, Kuem Ju ; Kim, Sung Jin ; Kim, Suk Won ; Choi, Song Hyen ; Shin, You Chan ; Park, Sang Ha ; Moon, Bo Hyun ; Cho, Eujin ; Lee, Min-Soo ; Choi, Sang-Hyun ; Chun, Boe Gwun ; Shin, Kyung-Ho. / Chronic mild stress decreases survival, but not proliferation, of new-born cells in adult rat hippocampus. In: Experimental and Molecular Medicine. 2006 ; Vol. 38, No. 1. pp. 44-54.
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