Chronic mild stress decreases survival, but not proliferation, of new-born cells in adult rat hippocampus

Kuem Ju Lee, Sung Jin Kim, Suk Won Kim, Song Hyen Choi, You Chan Shin, Sang Ha Park, Bo Hyun Moon, Eujin Cho, Min Soo Lee, Sang Hyun Choi, Boe Gwun Chun, Kyung Ho Shin

Research output: Contribution to journalArticlepeer-review

98 Citations (Scopus)


New-born cells continue to proliferate and survive to become mature granule cells in adult rat hippocampus. Although this process, known as neurogenesis, is inhibited by acute stress, it is not clear whether chronic stress affects neurogenesis. To determine whether chronic mild stress (CMS) influences neurogenesis in the adult rat hippocampus, male Sprague-Dawley rats were exposed to CMS and administered bromodeoxyuridine (BrdU) before or after CMS to observe the survival/differentiation or proliferation of new-born cells, respectively. In addition, we measured brain-derived neurotrophic factor (BDNF) mRNA in the granule cell layer (GCL) of the hippocampus, because BDNF is known to play an important role in the survival of new-born cells. CMS significantly decreased the survival of newborn cells in the GCL, but did not influence the proliferation or differentiation of new-born cells. CMS did not affect the proliferation and survival of new-born cells in the hilus. In addition, CMS did not change BDNF mRNA levels in the GCL. These results demonstrate that CMS reduces the survival of new-born cells but not of their proliferation, suggesting that repeated mild stress could influence a part of neurogenesis, but not the whole part of neurogenesis. These results raise the possibility that the survival of new-born cells may be suppressed in the presence of normal BDNF mRNA levels in GCL.

Original languageEnglish
Pages (from-to)44-54
Number of pages11
JournalExperimental and Molecular Medicine
Issue number1
Publication statusPublished - 2006 Feb 28
Externally publishedYes


  • Brain-derived neurotrophic factor
  • Bromodeoxyuridine
  • Depression
  • Hippocampus
  • Neurogenesis
  • Stress

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry


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