Chryseobacterium kwangjuense sp. nov., isolated from pepper (Capsicum annuum L.) root

Mee Kyung Sang, Hye Sook Kim, Inn Shik Myung, Choong Min Ryu, Beom Seok Kim, Ki Deok Kim

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The yellow-pigmented, Gram-stain-negative, rod-shaped bacterium KJ1R5T was isolated from the root of a pepper plant grown in a field in Kwangju, Korea. Strain KJ1R5T was characterized by physiological, biochemical, and molecular genetic analyses. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KJ1R5T was most closely related to members of the genus Chryseobacterium, and that the strain exhibited the highest similarities with type strains of Chryseobacterium vrystaatense (97.0 %) and Chryseobacterium rhizosphaerae (97.1 %). DNA-DNA hybridization reassociation values between strain KJ1R5T and type strains of C. vrystaatense KACC 11675T and C. rhizosphaerae KACC 14918T were 46.9 and 38.4 %, respectively. The DNA G+C content of KJ1R5T is 40.2 mol%. The predominant respiratory quinone of KJ1R5T was menaquinone MK-6; major cellular fatty acids were iso-C15: 0, summed feature 3 (C16: 1ω7c and/or C16: 1ω6c), iso-C17: 1ω9c, and iso-C17: 03-OH. On the basis of these phenotypic and genotypic characteristics, the strain significantly differed from representative strains belonging to the genus Chryseobacterium. Thus, we propose that strain KJ1R5T represents a novel species of the genus Chryseobacterium, named Chryseobacterium kwangjuense sp. nov. The type strain is KJ1R5T (= KACC 13029T = JCM 15904T).

Original languageEnglish
Pages (from-to)2835-2840
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART8
DOIs
Publication statusPublished - 2013 Aug 1

Fingerprint

Chryseobacterium
Capsicum
Capsicum annuum
pepper
DNA
new species
Molecular Biology
fatty acid
phylogenetics
bacterium
gene
molecular genetics
Base Composition
Korea
rRNA Genes
Vitamin K 2
Fatty Acids
menaquinones
Bacteria
quinones

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Chryseobacterium kwangjuense sp. nov., isolated from pepper (Capsicum annuum L.) root. / Sang, Mee Kyung; Kim, Hye Sook; Myung, Inn Shik; Ryu, Choong Min; Kim, Beom Seok; Kim, Ki Deok.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 63, No. PART8, 01.08.2013, p. 2835-2840.

Research output: Contribution to journalArticle

@article{50353f0362bc42e4bd996c4369dbca7b,
title = "Chryseobacterium kwangjuense sp. nov., isolated from pepper (Capsicum annuum L.) root",
abstract = "The yellow-pigmented, Gram-stain-negative, rod-shaped bacterium KJ1R5T was isolated from the root of a pepper plant grown in a field in Kwangju, Korea. Strain KJ1R5T was characterized by physiological, biochemical, and molecular genetic analyses. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KJ1R5T was most closely related to members of the genus Chryseobacterium, and that the strain exhibited the highest similarities with type strains of Chryseobacterium vrystaatense (97.0 {\%}) and Chryseobacterium rhizosphaerae (97.1 {\%}). DNA-DNA hybridization reassociation values between strain KJ1R5T and type strains of C. vrystaatense KACC 11675T and C. rhizosphaerae KACC 14918T were 46.9 and 38.4 {\%}, respectively. The DNA G+C content of KJ1R5T is 40.2 mol{\%}. The predominant respiratory quinone of KJ1R5T was menaquinone MK-6; major cellular fatty acids were iso-C15: 0, summed feature 3 (C16: 1ω7c and/or C16: 1ω6c), iso-C17: 1ω9c, and iso-C17: 03-OH. On the basis of these phenotypic and genotypic characteristics, the strain significantly differed from representative strains belonging to the genus Chryseobacterium. Thus, we propose that strain KJ1R5T represents a novel species of the genus Chryseobacterium, named Chryseobacterium kwangjuense sp. nov. The type strain is KJ1R5T (= KACC 13029T = JCM 15904T).",
author = "Sang, {Mee Kyung} and Kim, {Hye Sook} and Myung, {Inn Shik} and Ryu, {Choong Min} and Kim, {Beom Seok} and Kim, {Ki Deok}",
year = "2013",
month = "8",
day = "1",
doi = "10.1099/ijs.0.048496-0",
language = "English",
volume = "63",
pages = "2835--2840",
journal = "The BMJ",
issn = "0730-6512",
publisher = "Kluwer Academic Publishers",
number = "PART8",

}

TY - JOUR

T1 - Chryseobacterium kwangjuense sp. nov., isolated from pepper (Capsicum annuum L.) root

AU - Sang, Mee Kyung

AU - Kim, Hye Sook

AU - Myung, Inn Shik

AU - Ryu, Choong Min

AU - Kim, Beom Seok

AU - Kim, Ki Deok

PY - 2013/8/1

Y1 - 2013/8/1

N2 - The yellow-pigmented, Gram-stain-negative, rod-shaped bacterium KJ1R5T was isolated from the root of a pepper plant grown in a field in Kwangju, Korea. Strain KJ1R5T was characterized by physiological, biochemical, and molecular genetic analyses. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KJ1R5T was most closely related to members of the genus Chryseobacterium, and that the strain exhibited the highest similarities with type strains of Chryseobacterium vrystaatense (97.0 %) and Chryseobacterium rhizosphaerae (97.1 %). DNA-DNA hybridization reassociation values between strain KJ1R5T and type strains of C. vrystaatense KACC 11675T and C. rhizosphaerae KACC 14918T were 46.9 and 38.4 %, respectively. The DNA G+C content of KJ1R5T is 40.2 mol%. The predominant respiratory quinone of KJ1R5T was menaquinone MK-6; major cellular fatty acids were iso-C15: 0, summed feature 3 (C16: 1ω7c and/or C16: 1ω6c), iso-C17: 1ω9c, and iso-C17: 03-OH. On the basis of these phenotypic and genotypic characteristics, the strain significantly differed from representative strains belonging to the genus Chryseobacterium. Thus, we propose that strain KJ1R5T represents a novel species of the genus Chryseobacterium, named Chryseobacterium kwangjuense sp. nov. The type strain is KJ1R5T (= KACC 13029T = JCM 15904T).

AB - The yellow-pigmented, Gram-stain-negative, rod-shaped bacterium KJ1R5T was isolated from the root of a pepper plant grown in a field in Kwangju, Korea. Strain KJ1R5T was characterized by physiological, biochemical, and molecular genetic analyses. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KJ1R5T was most closely related to members of the genus Chryseobacterium, and that the strain exhibited the highest similarities with type strains of Chryseobacterium vrystaatense (97.0 %) and Chryseobacterium rhizosphaerae (97.1 %). DNA-DNA hybridization reassociation values between strain KJ1R5T and type strains of C. vrystaatense KACC 11675T and C. rhizosphaerae KACC 14918T were 46.9 and 38.4 %, respectively. The DNA G+C content of KJ1R5T is 40.2 mol%. The predominant respiratory quinone of KJ1R5T was menaquinone MK-6; major cellular fatty acids were iso-C15: 0, summed feature 3 (C16: 1ω7c and/or C16: 1ω6c), iso-C17: 1ω9c, and iso-C17: 03-OH. On the basis of these phenotypic and genotypic characteristics, the strain significantly differed from representative strains belonging to the genus Chryseobacterium. Thus, we propose that strain KJ1R5T represents a novel species of the genus Chryseobacterium, named Chryseobacterium kwangjuense sp. nov. The type strain is KJ1R5T (= KACC 13029T = JCM 15904T).

UR - http://www.scopus.com/inward/record.url?scp=84880967966&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880967966&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.048496-0

DO - 10.1099/ijs.0.048496-0

M3 - Article

VL - 63

SP - 2835

EP - 2840

JO - The BMJ

JF - The BMJ

SN - 0730-6512

IS - PART8

ER -