Chryseobacterium phosphatilyticum sp. nov., a phosphate-solubilizing endophyte isolated from cucumber (Cucumis sativus L.) root

Jin Ju Jeong, Mee Kyung Sang, Dong Wan Lee, In Geol Choi, Ki Deok Kim

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A bacterial strain, designated as ISE14 T , with Gram-stain-negative and non-motile rod-shaped cells, was isolated from the root of a cucumber plant collected in a field in Iksan, Republic of Korea and was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISE14 T represented a member of the genus Chryseobacterium and was closely related to Chryseobacterium viscerum 687B-08 T (16S rRNA gene sequence similarity of 98.50 %), Chryseobacterium lactis NCTC 11390 T (98.49 %), Chryseobacterium ureilyticum F-Fue-04IIIaaaa T (98.49 %) and Chryseobacterium oncorhynchi 701B-08 T (98.04 %). Average nucleotide identity values between genome sequences of strain ISE14 T and the closely related species ranged from 81.44 to 83.15 %, which were lower than the threshold of 95 % (corresponding to a DNA–DNA hybridization value of 70 %). The DNA G+C content of strain ISE14 T was 36.3 mol%. The dominant fatty acids were iso-C 15: 0 , summed feature 9 (iso-C 17: 1 !9c and/or C 16: 0 10-methyl), summed feature 3 (iso-C 15: 0 2-OH and/or C 16: 1 !7c) and iso-C 17: 0 3-OH. The major polar lipids were phosphatidylethanolamine, three unidentified aminolipids and eight unidentified lipids; the predominant respiratory quinone was MK-6. On the basis of the evidence presented in this study, strain ISE14 T can be distinguished from closely related species belonging to the genus Chryseobacterium. Thus, strain ISE14 T is a novel species of the genus Chryseobacterium, for which the name Chryseobacterium phosphatilyticum sp. nov. is proposed. The type strain is ISE14 T (=KACC 19820 T =JCM 32876 T ).

Original languageEnglish
Article number003091
Pages (from-to)610-615
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume69
Issue number3
DOIs
Publication statusPublished - 2019 Mar

Keywords

  • Average nucleotide identity
  • Biological control
  • Chryseobacterium phosphatilyticum
  • Phosphate solubilizer
  • Phytophthora blight

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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