CIIA induces the epithelial-mesenchymal transition and cell invasion

Sun Young Han, Hyun Sub Hwang, Ji Soo Chae, Suk Jin Yang, Je Hyun Yoon, Young Il Yeom, Eui Ju Choi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Epithelial-mesenchymal transition (EMT) and the acquisition of invasive potential are key events in tumor progression. We now show that CIIA, originally identified as an anti-apoptotic protein, induces the EMT and promotes cell migration and invasion. Ectopic expression of CIIA induced down-regulation of E-cadherin and claudin-1 as well as up-regulation of N-cadherin in MDCK cells. It also disrupted the differentiated epithelial morphology of MDCK cells grown in three-dimensional Matrigel cultures as well as increased the migration and invasion of MDCK cells in vitro. Furthermore, depletion of endogenous CIIA by RNA interference inhibited the migration and invasion of HeLa cells, and this inhibition was abolished by RNA interference-mediated depletion of claudin-1. These results suggest that CIIA functions as an inducer of cell invasion, and this effect is mediated, at least in part, through down-regulation of claudin-1.

Original languageEnglish
Pages (from-to)548-552
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume387
Issue number3
DOIs
Publication statusPublished - 2009 Sep 25

Fingerprint

Claudin-1
Madin Darby Canine Kidney Cells
Epithelial-Mesenchymal Transition
Cadherins
RNA Interference
Down-Regulation
RNA
Apoptosis Regulatory Proteins
Helper-Inducer T-Lymphocytes
HeLa Cells
Cell culture
Cell Movement
Tumors
Up-Regulation
Cells
Neoplasms

Keywords

  • CIIA
  • Claudin-1
  • Epithelial-mesenchymal transition
  • Invasion
  • Migration

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

CIIA induces the epithelial-mesenchymal transition and cell invasion. / Han, Sun Young; Hwang, Hyun Sub; Chae, Ji Soo; Yang, Suk Jin; Yoon, Je Hyun; Yeom, Young Il; Choi, Eui Ju.

In: Biochemical and Biophysical Research Communications, Vol. 387, No. 3, 25.09.2009, p. 548-552.

Research output: Contribution to journalArticle

Han, Sun Young ; Hwang, Hyun Sub ; Chae, Ji Soo ; Yang, Suk Jin ; Yoon, Je Hyun ; Yeom, Young Il ; Choi, Eui Ju. / CIIA induces the epithelial-mesenchymal transition and cell invasion. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 387, No. 3. pp. 548-552.
@article{d51a2df47b364610b5986af1f2a7cce5,
title = "CIIA induces the epithelial-mesenchymal transition and cell invasion",
abstract = "Epithelial-mesenchymal transition (EMT) and the acquisition of invasive potential are key events in tumor progression. We now show that CIIA, originally identified as an anti-apoptotic protein, induces the EMT and promotes cell migration and invasion. Ectopic expression of CIIA induced down-regulation of E-cadherin and claudin-1 as well as up-regulation of N-cadherin in MDCK cells. It also disrupted the differentiated epithelial morphology of MDCK cells grown in three-dimensional Matrigel cultures as well as increased the migration and invasion of MDCK cells in vitro. Furthermore, depletion of endogenous CIIA by RNA interference inhibited the migration and invasion of HeLa cells, and this inhibition was abolished by RNA interference-mediated depletion of claudin-1. These results suggest that CIIA functions as an inducer of cell invasion, and this effect is mediated, at least in part, through down-regulation of claudin-1.",
keywords = "CIIA, Claudin-1, Epithelial-mesenchymal transition, Invasion, Migration",
author = "Han, {Sun Young} and Hwang, {Hyun Sub} and Chae, {Ji Soo} and Yang, {Suk Jin} and Yoon, {Je Hyun} and Yeom, {Young Il} and Choi, {Eui Ju}",
year = "2009",
month = "9",
day = "25",
doi = "10.1016/j.bbrc.2009.07.050",
language = "English",
volume = "387",
pages = "548--552",
journal = "The BMJ",
issn = "0730-6512",
publisher = "Kluwer Academic Publishers",
number = "3",

}

TY - JOUR

T1 - CIIA induces the epithelial-mesenchymal transition and cell invasion

AU - Han, Sun Young

AU - Hwang, Hyun Sub

AU - Chae, Ji Soo

AU - Yang, Suk Jin

AU - Yoon, Je Hyun

AU - Yeom, Young Il

AU - Choi, Eui Ju

PY - 2009/9/25

Y1 - 2009/9/25

N2 - Epithelial-mesenchymal transition (EMT) and the acquisition of invasive potential are key events in tumor progression. We now show that CIIA, originally identified as an anti-apoptotic protein, induces the EMT and promotes cell migration and invasion. Ectopic expression of CIIA induced down-regulation of E-cadherin and claudin-1 as well as up-regulation of N-cadherin in MDCK cells. It also disrupted the differentiated epithelial morphology of MDCK cells grown in three-dimensional Matrigel cultures as well as increased the migration and invasion of MDCK cells in vitro. Furthermore, depletion of endogenous CIIA by RNA interference inhibited the migration and invasion of HeLa cells, and this inhibition was abolished by RNA interference-mediated depletion of claudin-1. These results suggest that CIIA functions as an inducer of cell invasion, and this effect is mediated, at least in part, through down-regulation of claudin-1.

AB - Epithelial-mesenchymal transition (EMT) and the acquisition of invasive potential are key events in tumor progression. We now show that CIIA, originally identified as an anti-apoptotic protein, induces the EMT and promotes cell migration and invasion. Ectopic expression of CIIA induced down-regulation of E-cadherin and claudin-1 as well as up-regulation of N-cadherin in MDCK cells. It also disrupted the differentiated epithelial morphology of MDCK cells grown in three-dimensional Matrigel cultures as well as increased the migration and invasion of MDCK cells in vitro. Furthermore, depletion of endogenous CIIA by RNA interference inhibited the migration and invasion of HeLa cells, and this inhibition was abolished by RNA interference-mediated depletion of claudin-1. These results suggest that CIIA functions as an inducer of cell invasion, and this effect is mediated, at least in part, through down-regulation of claudin-1.

KW - CIIA

KW - Claudin-1

KW - Epithelial-mesenchymal transition

KW - Invasion

KW - Migration

UR - http://www.scopus.com/inward/record.url?scp=68249088729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=68249088729&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.07.050

DO - 10.1016/j.bbrc.2009.07.050

M3 - Article

VL - 387

SP - 548

EP - 552

JO - The BMJ

JF - The BMJ

SN - 0730-6512

IS - 3

ER -