Abstract
Objectives: To compare the frequency and absolute numbers of circulating endothelial progenitor cells (EPCs) in healthy control subjects and patients with gynaecological cancer, and to test the hypothesis that cancer treatment lowers EPC numbers. Methods: Patients with cervical or ovarian cancer and healthy control subjects provided peripheral blood samples for the isolation of mononuclear cells. EPCs were identified by quadruple immunofluorescence staining and flow cytometry as CD45-/CD34+/CD133+/vascular endothelial growth factor receptor 2 (VEGFR2)+ cells. Results: In total, 28 participants were enrolled. Circulating EPCs were present at higher frequencies (and in greater absolute numbers) in patients with cervical or ovarian cancer (n=14) than in controls (n=14). Concurrent chemoradiation therapy or surgery significantly reduced the frequency and number of EPCs in patients with gynaecological cancer, compared with pretreatment levels. Conclusions: EPC levels decline throughout cancer treatment; their measurement may therefore be a useful surrogate marker to monitor treatment response.
| Original language | English |
|---|---|
| Pages (from-to) | 293-299 |
| Number of pages | 7 |
| Journal | Journal of International Medical Research |
| Volume | 41 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2013 Jun 4 |
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Keywords
- CD133
- CD34
- CD45
- Cervical cancer
- Circulating endothelial progenitor cells
- Ovarian cancer
- Vegfr2
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Biochemistry, medical
Cite this
Circulating endothelial progenitor cells in gynaecological cancer. / Kim, Yoon Byoung; Chung, Ye Won; Bae, Hyo Sook; Lee, Jae Kwan; Lee, Nak Woo; Lee, Kyu Wan; Song, Jae Yun.
In: Journal of International Medical Research, Vol. 41, No. 2, 04.06.2013, p. 293-299.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Circulating endothelial progenitor cells in gynaecological cancer
AU - Kim, Yoon Byoung
AU - Chung, Ye Won
AU - Bae, Hyo Sook
AU - Lee, Jae Kwan
AU - Lee, Nak Woo
AU - Lee, Kyu Wan
AU - Song, Jae Yun
PY - 2013/6/4
Y1 - 2013/6/4
N2 - Objectives: To compare the frequency and absolute numbers of circulating endothelial progenitor cells (EPCs) in healthy control subjects and patients with gynaecological cancer, and to test the hypothesis that cancer treatment lowers EPC numbers. Methods: Patients with cervical or ovarian cancer and healthy control subjects provided peripheral blood samples for the isolation of mononuclear cells. EPCs were identified by quadruple immunofluorescence staining and flow cytometry as CD45-/CD34+/CD133+/vascular endothelial growth factor receptor 2 (VEGFR2)+ cells. Results: In total, 28 participants were enrolled. Circulating EPCs were present at higher frequencies (and in greater absolute numbers) in patients with cervical or ovarian cancer (n=14) than in controls (n=14). Concurrent chemoradiation therapy or surgery significantly reduced the frequency and number of EPCs in patients with gynaecological cancer, compared with pretreatment levels. Conclusions: EPC levels decline throughout cancer treatment; their measurement may therefore be a useful surrogate marker to monitor treatment response.
AB - Objectives: To compare the frequency and absolute numbers of circulating endothelial progenitor cells (EPCs) in healthy control subjects and patients with gynaecological cancer, and to test the hypothesis that cancer treatment lowers EPC numbers. Methods: Patients with cervical or ovarian cancer and healthy control subjects provided peripheral blood samples for the isolation of mononuclear cells. EPCs were identified by quadruple immunofluorescence staining and flow cytometry as CD45-/CD34+/CD133+/vascular endothelial growth factor receptor 2 (VEGFR2)+ cells. Results: In total, 28 participants were enrolled. Circulating EPCs were present at higher frequencies (and in greater absolute numbers) in patients with cervical or ovarian cancer (n=14) than in controls (n=14). Concurrent chemoradiation therapy or surgery significantly reduced the frequency and number of EPCs in patients with gynaecological cancer, compared with pretreatment levels. Conclusions: EPC levels decline throughout cancer treatment; their measurement may therefore be a useful surrogate marker to monitor treatment response.
KW - CD133
KW - CD34
KW - CD45
KW - Cervical cancer
KW - Circulating endothelial progenitor cells
KW - Ovarian cancer
KW - Vegfr2
UR - http://www.scopus.com/inward/record.url?scp=84878348242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84878348242&partnerID=8YFLogxK
U2 - 10.1177/0300060513476999
DO - 10.1177/0300060513476999
M3 - Article
C2 - 23569031
AN - SCOPUS:84878348242
VL - 41
SP - 293
EP - 299
JO - Journal of International Medical Research
JF - Journal of International Medical Research
SN - 0300-0605
IS - 2
ER -