CKβ8/CCL23 and its isoform CKβ8-1 induce up-regulation of cyclins via the Gi/Go protein/PLC/PKCδ/ERK leading to cell-cycle progression

Jeonghan Kim, Yoon Suk Kim, Jesang Ko

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

CKβ8/CCL23 is a CC chemokine and alternative splicing of the CKβ8 gene produces two mRNAs that encode CKβ8 and its isoform CKβ8-1. Chemokines play a critical role in leukocyte trafficking and development of inflammation and chemokines are also known to be involved in cell proliferation. To investigate participation of CKβ8 and CKβ8-1 in cell proliferation, we examined the effects of CKβ8 and CKβ8-1 in the cell cycle. Both CKβ8 and CKβ8-1 induced cell-cycle progression. We next investigated whether MAPKs are involved in CKβ8- and CKβ8-1-induced cell proliferation. CKβ8- and CKβ8-1-stimulated cells showed phosphorylation of ERK1/2 and an inhibitor study indicated that CKβ8- and CKβ8-1-induced activation of ERK1/2 is mediated by the Gi/Go protein, PLC, and PKCδ. CKβ8 and CKβ8-1 regulated expression of the cell cycle regulators cyclin D3 and cyclin B1, and the immediate early response gene products c-Myc and Egr-1. These results indicate that both CKβ8 and CKβ8-1 are involved in cell proliferation by modulating the cell cycle regulators.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalCytokine
Volume50
Issue number1
DOIs
Publication statusPublished - 2010 Apr

Keywords

  • CKβ8
  • CKβ8-1
  • Cell cycle
  • Cyclin
  • Signal transduction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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