Class II β-tubulin is a novel marker for human tonsillar M cells and follicular dendritic cells

Ju Han Lee, Su Kang Kong, Zheng Sheng Wu, Qiang Wu, Jongseon Choe, Hyun Yee Cho, Seung Yeon Ha, Sun Ho Kee, Young Sik Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: Membranous (M) cell of the human palatine tonsil is an antigen entry site for mucosal infection, but its location is obscure in histological sections. Recently, a microarray analysis has demonstrated that clusterin, annexin A5, CD44, MMP14, and β-tubulin are candidate genes of M cell marker in mice. Among these genes, we here describe class II β-tubulin as a new marker for human tonsillar M cells and follicular dendritic cells (FDCs), and present its usefulness for diagnosis of angioimmunoblastic T-cell lymphomas (AILTs). Materials and methods: Immunohistochemistry and Western blotting for class II β-tubulin were performed using 81 cases of lymphoid, gastrointestinal and thyroid tissues, and an FDC cell line, respectively. Double immunostaining with clusterin and class II β-tubulin were carried out. Results: Class II β-tubulin localized the M cells and FDCs in the palatine tonsils (10/10, 100%) and adenoids (10/10, 100%). It was colocalized with clusterin in the palatine tonsils. However, class II β-tubulin staining did not identify intestinal M cells in the intestines. Immunoblot analysis revealed that class II β-tubulin expression was upregulated in HK cells, a normal FDC cell line. Class II β-tubulin immunostaining highlighted hyperplastic FDC meshworks in all AILTs (14/14, 100%). Conclusion: Class II β-tubulin is a specific histochemical marker for human tonsillar M cells and FDCs. Thus, class II β-tubulin immunostaining may be useful to identify tonsillar M cells and to diagnose FDC proliferative lesions such as AILT.

Original languageEnglish
Pages (from-to)533-539
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume39
Issue number7
DOIs
Publication statusPublished - 2010 Aug

Keywords

  • M cell
  • follicular dendritic cell
  • palatine tonsil
  • tubulin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

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