Class II β-tubulin is a novel marker for human tonsillar M cells and follicular dendritic cells

Ju-Han Lee, Su Kang Kong, Zheng Sheng Wu, Qiang Wu, Jongseon Choe, Hyun Yee Cho, Seung Yeon Ha, Sun-Ho Kee, Young Sik Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Objective: Membranous (M) cell of the human palatine tonsil is an antigen entry site for mucosal infection, but its location is obscure in histological sections. Recently, a microarray analysis has demonstrated that clusterin, annexin A5, CD44, MMP14, and β-tubulin are candidate genes of M cell marker in mice. Among these genes, we here describe class II β-tubulin as a new marker for human tonsillar M cells and follicular dendritic cells (FDCs), and present its usefulness for diagnosis of angioimmunoblastic T-cell lymphomas (AILTs). Materials and methods: Immunohistochemistry and Western blotting for class II β-tubulin were performed using 81 cases of lymphoid, gastrointestinal and thyroid tissues, and an FDC cell line, respectively. Double immunostaining with clusterin and class II β-tubulin were carried out. Results: Class II β-tubulin localized the M cells and FDCs in the palatine tonsils (10/10, 100%) and adenoids (10/10, 100%). It was colocalized with clusterin in the palatine tonsils. However, class II β-tubulin staining did not identify intestinal M cells in the intestines. Immunoblot analysis revealed that class II β-tubulin expression was upregulated in HK cells, a normal FDC cell line. Class II β-tubulin immunostaining highlighted hyperplastic FDC meshworks in all AILTs (14/14, 100%). Conclusion: Class II β-tubulin is a specific histochemical marker for human tonsillar M cells and FDCs. Thus, class II β-tubulin immunostaining may be useful to identify tonsillar M cells and to diagnose FDC proliferative lesions such as AILT.

Original languageEnglish
Pages (from-to)533-539
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume39
Issue number7
DOIs
Publication statusPublished - 2010 Aug 1

Fingerprint

Follicular Dendritic Cells
Tubulin
Clusterin
Palatine Tonsil
Adenoids
Cell Line
MHC Class II Genes
T-Cell Lymphoma
Annexin A5
Microarray Analysis
Intestines
Western Blotting
Immunohistochemistry

Keywords

  • follicular dendritic cell
  • M cell
  • palatine tonsil
  • tubulin

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics

Cite this

Class II β-tubulin is a novel marker for human tonsillar M cells and follicular dendritic cells. / Lee, Ju-Han; Kong, Su Kang; Wu, Zheng Sheng; Wu, Qiang; Choe, Jongseon; Cho, Hyun Yee; Ha, Seung Yeon; Kee, Sun-Ho; Kim, Young Sik.

In: Journal of Oral Pathology and Medicine, Vol. 39, No. 7, 01.08.2010, p. 533-539.

Research output: Contribution to journalArticle

Lee, Ju-Han ; Kong, Su Kang ; Wu, Zheng Sheng ; Wu, Qiang ; Choe, Jongseon ; Cho, Hyun Yee ; Ha, Seung Yeon ; Kee, Sun-Ho ; Kim, Young Sik. / Class II β-tubulin is a novel marker for human tonsillar M cells and follicular dendritic cells. In: Journal of Oral Pathology and Medicine. 2010 ; Vol. 39, No. 7. pp. 533-539.
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AU - Choe, Jongseon

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AU - Ha, Seung Yeon

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AU - Kim, Young Sik

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N2 - Objective: Membranous (M) cell of the human palatine tonsil is an antigen entry site for mucosal infection, but its location is obscure in histological sections. Recently, a microarray analysis has demonstrated that clusterin, annexin A5, CD44, MMP14, and β-tubulin are candidate genes of M cell marker in mice. Among these genes, we here describe class II β-tubulin as a new marker for human tonsillar M cells and follicular dendritic cells (FDCs), and present its usefulness for diagnosis of angioimmunoblastic T-cell lymphomas (AILTs). Materials and methods: Immunohistochemistry and Western blotting for class II β-tubulin were performed using 81 cases of lymphoid, gastrointestinal and thyroid tissues, and an FDC cell line, respectively. Double immunostaining with clusterin and class II β-tubulin were carried out. Results: Class II β-tubulin localized the M cells and FDCs in the palatine tonsils (10/10, 100%) and adenoids (10/10, 100%). It was colocalized with clusterin in the palatine tonsils. However, class II β-tubulin staining did not identify intestinal M cells in the intestines. Immunoblot analysis revealed that class II β-tubulin expression was upregulated in HK cells, a normal FDC cell line. Class II β-tubulin immunostaining highlighted hyperplastic FDC meshworks in all AILTs (14/14, 100%). Conclusion: Class II β-tubulin is a specific histochemical marker for human tonsillar M cells and FDCs. Thus, class II β-tubulin immunostaining may be useful to identify tonsillar M cells and to diagnose FDC proliferative lesions such as AILT.

AB - Objective: Membranous (M) cell of the human palatine tonsil is an antigen entry site for mucosal infection, but its location is obscure in histological sections. Recently, a microarray analysis has demonstrated that clusterin, annexin A5, CD44, MMP14, and β-tubulin are candidate genes of M cell marker in mice. Among these genes, we here describe class II β-tubulin as a new marker for human tonsillar M cells and follicular dendritic cells (FDCs), and present its usefulness for diagnosis of angioimmunoblastic T-cell lymphomas (AILTs). Materials and methods: Immunohistochemistry and Western blotting for class II β-tubulin were performed using 81 cases of lymphoid, gastrointestinal and thyroid tissues, and an FDC cell line, respectively. Double immunostaining with clusterin and class II β-tubulin were carried out. Results: Class II β-tubulin localized the M cells and FDCs in the palatine tonsils (10/10, 100%) and adenoids (10/10, 100%). It was colocalized with clusterin in the palatine tonsils. However, class II β-tubulin staining did not identify intestinal M cells in the intestines. Immunoblot analysis revealed that class II β-tubulin expression was upregulated in HK cells, a normal FDC cell line. Class II β-tubulin immunostaining highlighted hyperplastic FDC meshworks in all AILTs (14/14, 100%). Conclusion: Class II β-tubulin is a specific histochemical marker for human tonsillar M cells and FDCs. Thus, class II β-tubulin immunostaining may be useful to identify tonsillar M cells and to diagnose FDC proliferative lesions such as AILT.

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