Cleavage-induced fluorescence change via hydrophilicity control: A new strategy for biological application

Chang Keun Lim; Jin Seong Lee; Tai Hwan Ha; Ick Chan Kwon; Cheol Hee Ahn; Soo Young Park

doi:10.1016/j.jphotochem.2006.12.002

Cleavage-induced fluorescence change via hydrophilicity control: A new strategy for biological application

Chang Keun Lim, Jin Seong Lee, Tai Hwan Ha, Ick Chan Kwon, Cheol Hee Ahn, Soo Young Park

KU-KIST Graduate School of Converging Science and Technology

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

In this work, we report a novel stimuli-sensitive probe working on the principle of the cleavage-induced fluorescent change via hydrophilicity control. In our Si-Py-Ac-PEG probe, cleavage of hydrophilic PEG moiety in acidic environment induces hydrophobic interaction of pyrenes to increase their excimer emission. Our fluorescent probe (Si-Py-Ac-PEG) was successfully applied to the spotting microarray and nanoparticle suspension to demonstrate the potential of biological applications, i.e. protein chip and bioimaging.

Original language	English
Pages (from-to)	149-154
Number of pages	6
Journal	Journal of Photochemistry and Photobiology A: Chemistry
Volume	188
Issue number	2-3
DOIs	https://doi.org/10.1016/j.jphotochem.2006.12.002
Publication status	Published - 2007 May 20

Keywords

Biomedical imaging
Cleavage
Excimer emission
Hydrophilicity control
Microarray

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)
Physics and Astronomy(all)

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10.1016/j.jphotochem.2006.12.002

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Cleavage-induced fluorescence change via hydrophilicity control : A new strategy for biological application. / Lim, Chang Keun; Lee, Jin Seong; Ha, Tai Hwan; Kwon, Ick Chan; Ahn, Cheol Hee; Park, Soo Young.

In: Journal of Photochemistry and Photobiology A: Chemistry, Vol. 188, No. 2-3, 20.05.2007, p. 149-154.

Research output: Contribution to journal › Article › peer-review

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abstract = "In this work, we report a novel stimuli-sensitive probe working on the principle of the cleavage-induced fluorescent change via hydrophilicity control. In our Si-Py-Ac-PEG probe, cleavage of hydrophilic PEG moiety in acidic environment induces hydrophobic interaction of pyrenes to increase their excimer emission. Our fluorescent probe (Si-Py-Ac-PEG) was successfully applied to the spotting microarray and nanoparticle suspension to demonstrate the potential of biological applications, i.e. protein chip and bioimaging.",

keywords = "Biomedical imaging, Cleavage, Excimer emission, Hydrophilicity control, Microarray",

author = "Lim, {Chang Keun} and Lee, {Jin Seong} and Ha, {Tai Hwan} and Kwon, {Ick Chan} and Ahn, {Cheol Hee} and Park, {Soo Young}",

note = "Funding Information: This work was supported by the Ministry of Science and Technology of Korea through National Research Laboratory (NRL) program awarded to Prof. Soo Young Park. Prof. C.-H. Ahn thanks to Next Generation New Technology Development Program (no. 10011352) for financial support. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

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T2 - A new strategy for biological application

AU - Lim, Chang Keun

AU - Lee, Jin Seong

AU - Ha, Tai Hwan

AU - Kwon, Ick Chan

AU - Ahn, Cheol Hee

AU - Park, Soo Young

N1 - Funding Information: This work was supported by the Ministry of Science and Technology of Korea through National Research Laboratory (NRL) program awarded to Prof. Soo Young Park. Prof. C.-H. Ahn thanks to Next Generation New Technology Development Program (no. 10011352) for financial support. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 2007/5/20

Y1 - 2007/5/20

N2 - In this work, we report a novel stimuli-sensitive probe working on the principle of the cleavage-induced fluorescent change via hydrophilicity control. In our Si-Py-Ac-PEG probe, cleavage of hydrophilic PEG moiety in acidic environment induces hydrophobic interaction of pyrenes to increase their excimer emission. Our fluorescent probe (Si-Py-Ac-PEG) was successfully applied to the spotting microarray and nanoparticle suspension to demonstrate the potential of biological applications, i.e. protein chip and bioimaging.

AB - In this work, we report a novel stimuli-sensitive probe working on the principle of the cleavage-induced fluorescent change via hydrophilicity control. In our Si-Py-Ac-PEG probe, cleavage of hydrophilic PEG moiety in acidic environment induces hydrophobic interaction of pyrenes to increase their excimer emission. Our fluorescent probe (Si-Py-Ac-PEG) was successfully applied to the spotting microarray and nanoparticle suspension to demonstrate the potential of biological applications, i.e. protein chip and bioimaging.

KW - Biomedical imaging

KW - Cleavage

KW - Excimer emission

KW - Hydrophilicity control

KW - Microarray

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JO - Journal of Photochemistry and Photobiology A: Chemistry

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Cleavage-induced fluorescence change via hydrophilicity control: A new strategy for biological application

Abstract

Keywords

ASJC Scopus subject areas

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