Cleavage-induced fluorescence change via hydrophilicity control: A new strategy for biological application

Chang Keun Lim, Jin Seong Lee, Tai Hwan Ha, Ick Chan Kwon, Cheol Hee Ahn, Soo Young Park

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In this work, we report a novel stimuli-sensitive probe working on the principle of the cleavage-induced fluorescent change via hydrophilicity control. In our Si-Py-Ac-PEG probe, cleavage of hydrophilic PEG moiety in acidic environment induces hydrophobic interaction of pyrenes to increase their excimer emission. Our fluorescent probe (Si-Py-Ac-PEG) was successfully applied to the spotting microarray and nanoparticle suspension to demonstrate the potential of biological applications, i.e. protein chip and bioimaging.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalJournal of Photochemistry and Photobiology A: Chemistry
Volume188
Issue number2-3
DOIs
Publication statusPublished - 2007 May 20
Externally publishedYes

Fingerprint

Hydrophilicity
Polyethylene glycols
cleavage
Fluorescence
fluorescence
probes
Pyrenes
Pyrene
pyrenes
excimers
Microarrays
Fluorescent Dyes
stimuli
Suspensions
chips
Nanoparticles
proteins
Proteins
nanoparticles
interactions

Keywords

  • Biomedical imaging
  • Cleavage
  • Excimer emission
  • Hydrophilicity control
  • Microarray

ASJC Scopus subject areas

  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Cleavage-induced fluorescence change via hydrophilicity control : A new strategy for biological application. / Lim, Chang Keun; Lee, Jin Seong; Ha, Tai Hwan; Kwon, Ick Chan; Ahn, Cheol Hee; Park, Soo Young.

In: Journal of Photochemistry and Photobiology A: Chemistry, Vol. 188, No. 2-3, 20.05.2007, p. 149-154.

Research output: Contribution to journalArticle

Lim, Chang Keun ; Lee, Jin Seong ; Ha, Tai Hwan ; Kwon, Ick Chan ; Ahn, Cheol Hee ; Park, Soo Young. / Cleavage-induced fluorescence change via hydrophilicity control : A new strategy for biological application. In: Journal of Photochemistry and Photobiology A: Chemistry. 2007 ; Vol. 188, No. 2-3. pp. 149-154.
@article{15d8090ee6824d14b36c3a7e196dd480,
title = "Cleavage-induced fluorescence change via hydrophilicity control: A new strategy for biological application",
abstract = "In this work, we report a novel stimuli-sensitive probe working on the principle of the cleavage-induced fluorescent change via hydrophilicity control. In our Si-Py-Ac-PEG probe, cleavage of hydrophilic PEG moiety in acidic environment induces hydrophobic interaction of pyrenes to increase their excimer emission. Our fluorescent probe (Si-Py-Ac-PEG) was successfully applied to the spotting microarray and nanoparticle suspension to demonstrate the potential of biological applications, i.e. protein chip and bioimaging.",
keywords = "Biomedical imaging, Cleavage, Excimer emission, Hydrophilicity control, Microarray",
author = "Lim, {Chang Keun} and Lee, {Jin Seong} and Ha, {Tai Hwan} and Kwon, {Ick Chan} and Ahn, {Cheol Hee} and Park, {Soo Young}",
year = "2007",
month = "5",
day = "20",
doi = "10.1016/j.jphotochem.2006.12.002",
language = "English",
volume = "188",
pages = "149--154",
journal = "Journal of Photochemistry and Photobiology A: Chemistry",
issn = "1010-6030",
publisher = "Elsevier",
number = "2-3",

}

TY - JOUR

T1 - Cleavage-induced fluorescence change via hydrophilicity control

T2 - A new strategy for biological application

AU - Lim, Chang Keun

AU - Lee, Jin Seong

AU - Ha, Tai Hwan

AU - Kwon, Ick Chan

AU - Ahn, Cheol Hee

AU - Park, Soo Young

PY - 2007/5/20

Y1 - 2007/5/20

N2 - In this work, we report a novel stimuli-sensitive probe working on the principle of the cleavage-induced fluorescent change via hydrophilicity control. In our Si-Py-Ac-PEG probe, cleavage of hydrophilic PEG moiety in acidic environment induces hydrophobic interaction of pyrenes to increase their excimer emission. Our fluorescent probe (Si-Py-Ac-PEG) was successfully applied to the spotting microarray and nanoparticle suspension to demonstrate the potential of biological applications, i.e. protein chip and bioimaging.

AB - In this work, we report a novel stimuli-sensitive probe working on the principle of the cleavage-induced fluorescent change via hydrophilicity control. In our Si-Py-Ac-PEG probe, cleavage of hydrophilic PEG moiety in acidic environment induces hydrophobic interaction of pyrenes to increase their excimer emission. Our fluorescent probe (Si-Py-Ac-PEG) was successfully applied to the spotting microarray and nanoparticle suspension to demonstrate the potential of biological applications, i.e. protein chip and bioimaging.

KW - Biomedical imaging

KW - Cleavage

KW - Excimer emission

KW - Hydrophilicity control

KW - Microarray

UR - http://www.scopus.com/inward/record.url?scp=34247126964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247126964&partnerID=8YFLogxK

U2 - 10.1016/j.jphotochem.2006.12.002

DO - 10.1016/j.jphotochem.2006.12.002

M3 - Article

AN - SCOPUS:34247126964

VL - 188

SP - 149

EP - 154

JO - Journal of Photochemistry and Photobiology A: Chemistry

JF - Journal of Photochemistry and Photobiology A: Chemistry

SN - 1010-6030

IS - 2-3

ER -