Clinical applications of in vivo fluorescence confocal laser scanning microscopy

Chil Hwan Oh, Sangyong Park, Junhyung Kim, Seunghan Ha, Gyuman Park, Gunwoo Lee, Onseok Lee, Byungseon Chun, Daegab Gweon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Living skin for basic and clinical research can be evaluated by Confocal Laser Scanning Microscope (CLSM) non-invasively. CLSM imaging system can achieve skin image its native state either "in vivo" or "fresh biopsy (ex vivo)" without fixation, sectioning and staining that is necessary for routine histology. This study examines the potential fluorescent CLSM with a various exogenous fluorescent contrast agent, to provide with more resolution images in skin. In addition, in vivo fluorescent CLSM researchers will be extended a range of potential clinical application. The prototype of our CLSM system has been developed by Prof. Gweon's group. The operating parameters are composed of some units, such as illuminated wavelength 488 nm, argon illumination power up to 20mW on the skin, objective lens, 0.9NA oil immersion, axial resolution 1.0μm, field of view 200μm x 100μm (lateral resolution, 0.3μm). In human volunteer, fluorescein sodium was administrated topically and intradermally. Animal studies were done in GFP transgenic mouse, IRC mouse and pig skin. For imaging of animal skin, fluorescein sodium, acridine orange, and curcumine were used for fluorescein contrast agent. We also used the GFP transgenic mouse for fluorescein CLSM imaging. In intact skin, absorption of fluorescein sodium by individual corneocyte and hair. Intradermal administrated the fluorescein sodium, distinct outline of keratinocyte cell border could be seen. Curcumin is a yellow food dye that has similar fluorescent properties to fluorescein sodium. Acridin Orange can be highlight nuclei in viable keratinocyte. In vivo CLSM of transgenic GFP mouse enable on in vivo, high resolution view of GFP expressing skin tissue. GFP signals are brightest in corneocyte, kertinocyte, hair and eccrine gland. In intact skin, absorption of fluorescein sodium by individual corneocyte and hair. Intradermal administrated the fluorescein sodium, distinct outline of keratinocyte cell border could be seen. In papillary dermis, fluorescein distribution is more homogeneous. Curcumin is a yellow food dye that has similar fluorescent properties to fluorescein sodium. In vivo CLSM of transgenic GFP mouse enable on in vivo, high resolution view of GFP expressing skin tissue. GFP signals are brightest in corneocyte, kertinocyte, skin appendage and blood vessels. In conclusion, this study demonstrates the usefulness of CLSM as technique for imaging skin in vivo. In addition, CLSM is non-invasive, the same tissue site may be imaged over a period of time to monitor the various change such as wound healing, severity of skin diseases and effect of therapeutic management.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VI
Volume6848
DOIs
Publication statusPublished - 2008 Apr 21
EventAdvanced Biomedical and Clinical Diagnostic Systems VI - San Jose, CA, United States
Duration: 2008 Jan 202008 Jan 21

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems VI
CountryUnited States
CitySan Jose, CA
Period08/1/2008/1/21

Keywords

  • Confocal laser scanning microscopy
  • In vivo
  • Non-invasive imaging
  • Skin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Oh, C. H., Park, S., Kim, J., Ha, S., Park, G., Lee, G., Lee, O., Chun, B., & Gweon, D. (2008). Clinical applications of in vivo fluorescence confocal laser scanning microscopy. In Advanced Biomedical and Clinical Diagnostic Systems VI (Vol. 6848). [684814] https://doi.org/10.1117/12.762866