Clinical characteristics and genotypes of rotaviruses in a neonatal intensive care unit

Jung Ok Shim, Dong Woo Son, So Yeon Shim, Eell Ryoo, Wonyong Kim, Yeon Chang Jung

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: There are few reports on the symptoms of rotavirus infections in neonates. This study aims to describe clinical signs of rotavirus infections among neonates, with a particular focus on preterm infants, and to show the distribution of genotypes in a neonatal intensive care unit (NICU). Methods: A prospective observational study was conducted at a regional NICU for 1 year. Stool specimens from every infant in the NICU were collected on admission, at weekly intervals, and from infants showing symptoms. Rotavirus antigens were detected by enzyme-linked immunosorbent assay (ELISA), and genotypes were confirmed by Reverse transcription-Polymerase chain reaction (RT-PCR). The infants were divided into three groups: symptomatic preterm infants with and without rotavirus-positive stools [Preterm(rota+) and Preterm(rota-), respectively] and symptomatic full- or near-term infants with rotavirus-positive stools [FT/NT(rota+)]. Demographic and outcome data were compared among these groups. Results: A total of 702 infants were evaluated for rotaviruses and 131 infants were included in this study. The prevalence of rotavirus infections was 25.2%. Preterm(rota+) differed from Preterm(rota-) and FT/NT(rota+) with respect to frequent feeding difficulty (p = 0.047 and 0.034, respectively) and higher percentage of neutropenia (p = 0.008 and 0.011, respectively). G4P[6] was the exclusive strain in both the Preterm(rota+) (97.7%) and FT/NT(rota+) (90.2%), and it was the same for nosocomial, institutional infections, and infections acquired at home. Conclusion: Systemic illness signs such as feeding difficulty and neutropenia are specific for preterm infants with rotavirus infections. G4P[6] was exclusive, regardless of preterm birth or locations of infections. This study might be helpful in developing policies for management and prevention of rotavirus infections in NICUs.

Original languageEnglish
Pages (from-to)18-23
Number of pages6
JournalPediatrics and Neonatology
Volume53
Issue number1
DOIs
Publication statusPublished - 2012 Feb 1
Externally publishedYes

Fingerprint

Rotavirus
Neonatal Intensive Care Units
Rotavirus Infections
Genotype
Premature Infants
Neutropenia
Newborn Infant
Premature Birth
Cross Infection
Infection
Reverse Transcription
Observational Studies
Enzyme-Linked Immunosorbent Assay
Demography
Prospective Studies
Antigens
Polymerase Chain Reaction

Keywords

  • clinical symptom
  • genotype
  • nosocomial
  • preterm infants
  • rotavirus

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Clinical characteristics and genotypes of rotaviruses in a neonatal intensive care unit. / Shim, Jung Ok; Son, Dong Woo; Shim, So Yeon; Ryoo, Eell; Kim, Wonyong; Jung, Yeon Chang.

In: Pediatrics and Neonatology, Vol. 53, No. 1, 01.02.2012, p. 18-23.

Research output: Contribution to journalArticle

Shim, Jung Ok ; Son, Dong Woo ; Shim, So Yeon ; Ryoo, Eell ; Kim, Wonyong ; Jung, Yeon Chang. / Clinical characteristics and genotypes of rotaviruses in a neonatal intensive care unit. In: Pediatrics and Neonatology. 2012 ; Vol. 53, No. 1. pp. 18-23.
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abstract = "Background: There are few reports on the symptoms of rotavirus infections in neonates. This study aims to describe clinical signs of rotavirus infections among neonates, with a particular focus on preterm infants, and to show the distribution of genotypes in a neonatal intensive care unit (NICU). Methods: A prospective observational study was conducted at a regional NICU for 1 year. Stool specimens from every infant in the NICU were collected on admission, at weekly intervals, and from infants showing symptoms. Rotavirus antigens were detected by enzyme-linked immunosorbent assay (ELISA), and genotypes were confirmed by Reverse transcription-Polymerase chain reaction (RT-PCR). The infants were divided into three groups: symptomatic preterm infants with and without rotavirus-positive stools [Preterm(rota+) and Preterm(rota-), respectively] and symptomatic full- or near-term infants with rotavirus-positive stools [FT/NT(rota+)]. Demographic and outcome data were compared among these groups. Results: A total of 702 infants were evaluated for rotaviruses and 131 infants were included in this study. The prevalence of rotavirus infections was 25.2{\%}. Preterm(rota+) differed from Preterm(rota-) and FT/NT(rota+) with respect to frequent feeding difficulty (p = 0.047 and 0.034, respectively) and higher percentage of neutropenia (p = 0.008 and 0.011, respectively). G4P[6] was the exclusive strain in both the Preterm(rota+) (97.7{\%}) and FT/NT(rota+) (90.2{\%}), and it was the same for nosocomial, institutional infections, and infections acquired at home. Conclusion: Systemic illness signs such as feeding difficulty and neutropenia are specific for preterm infants with rotavirus infections. G4P[6] was exclusive, regardless of preterm birth or locations of infections. This study might be helpful in developing policies for management and prevention of rotavirus infections in NICUs.",
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