Clinical effectiveness of urinary human chorionic gonadotropin related protein (hCGRP) quantification for diagnosis of ectopic pregnancy

Jae Kwan Lee, Min Jeong Oh, Joong Sik Shin, Kyung Joo Lee, Jung Hyun Nam, Jung Hak Cha, Jin Dong Chang, Dong Hee Cho, In Soo Kang, Paul I. Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We detected pregnancy related new molecule, human chorionic gonadotropin related protein (hCGRP) in the urine of a pregnant women by using a monoclonal antibody against the human chorionic gonadotropin (hCG). This study examined the effectiveness of urinary hCGRP quantification in diagnosing ectopic pregnancy. This study included 40 normal pregnant women and 25 patients with ectopic pregnancy. Patients' serum and urinary intact whole hCG (i-hCG) and hCGRP concentrations were measured using sandwich ELISA and the ratio of hCGRP to i-hCG was calculated. Statistical analysis was performed using statistical package for social sciences (SPSS) 10.0. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the cut-off value to discriminate ectopic pregnancies from normal intrauterine pregnancies. Urinary hCGRP and hCGRP/i-hCG ratio in ectopic pregnancy group (14±6.6 ng/mL, 4.6±1.9%, respectively) were significantly lower than those of normal pregnancy group (149±10.2 ng/mL, 29.7±1.9%, respectively; p<0.001). Based on ROC curve analysis, a cut-off point of urinary hCGRP/i-hCG ratio <16.2% discriminated between ectopic pregnancy and normal pregnancy with a sensitivity, specificity, positive predictive value and negative predictive value of 92.0%, 90.0%, 32.6%, and 99.5%, respectively. Urinary hCGRP/i-hCG ratio measurement may be effective in diagnosing ectopic pregnancy.

Original languageEnglish
Pages (from-to)461-467
Number of pages7
JournalJournal of Korean Medical Science
Volume20
Issue number3
Publication statusPublished - 2005 Jun 1

Fingerprint

Ectopic Pregnancy
Chorionic Gonadotropin
Proteins
Pregnancy
ROC Curve
Pregnant Women
Social Sciences
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Urine
Sensitivity and Specificity

Keywords

  • Chorionic Gonadotropin
  • Early Diagnosis
  • Ectopic
  • Human Chorionic Gonadotropin Related Protein
  • Pregnancy

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Clinical effectiveness of urinary human chorionic gonadotropin related protein (hCGRP) quantification for diagnosis of ectopic pregnancy. / Lee, Jae Kwan; Oh, Min Jeong; Shin, Joong Sik; Lee, Kyung Joo; Nam, Jung Hyun; Cha, Jung Hak; Chang, Jin Dong; Cho, Dong Hee; Kang, In Soo; Lee, Paul I.

In: Journal of Korean Medical Science, Vol. 20, No. 3, 01.06.2005, p. 461-467.

Research output: Contribution to journalArticle

Lee, Jae Kwan ; Oh, Min Jeong ; Shin, Joong Sik ; Lee, Kyung Joo ; Nam, Jung Hyun ; Cha, Jung Hak ; Chang, Jin Dong ; Cho, Dong Hee ; Kang, In Soo ; Lee, Paul I. / Clinical effectiveness of urinary human chorionic gonadotropin related protein (hCGRP) quantification for diagnosis of ectopic pregnancy. In: Journal of Korean Medical Science. 2005 ; Vol. 20, No. 3. pp. 461-467.
@article{5fef897f244245628221c280144248ef,
title = "Clinical effectiveness of urinary human chorionic gonadotropin related protein (hCGRP) quantification for diagnosis of ectopic pregnancy",
abstract = "We detected pregnancy related new molecule, human chorionic gonadotropin related protein (hCGRP) in the urine of a pregnant women by using a monoclonal antibody against the human chorionic gonadotropin (hCG). This study examined the effectiveness of urinary hCGRP quantification in diagnosing ectopic pregnancy. This study included 40 normal pregnant women and 25 patients with ectopic pregnancy. Patients' serum and urinary intact whole hCG (i-hCG) and hCGRP concentrations were measured using sandwich ELISA and the ratio of hCGRP to i-hCG was calculated. Statistical analysis was performed using statistical package for social sciences (SPSS) 10.0. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the cut-off value to discriminate ectopic pregnancies from normal intrauterine pregnancies. Urinary hCGRP and hCGRP/i-hCG ratio in ectopic pregnancy group (14±6.6 ng/mL, 4.6±1.9{\%}, respectively) were significantly lower than those of normal pregnancy group (149±10.2 ng/mL, 29.7±1.9{\%}, respectively; p<0.001). Based on ROC curve analysis, a cut-off point of urinary hCGRP/i-hCG ratio <16.2{\%} discriminated between ectopic pregnancy and normal pregnancy with a sensitivity, specificity, positive predictive value and negative predictive value of 92.0{\%}, 90.0{\%}, 32.6{\%}, and 99.5{\%}, respectively. Urinary hCGRP/i-hCG ratio measurement may be effective in diagnosing ectopic pregnancy.",
keywords = "Chorionic Gonadotropin, Early Diagnosis, Ectopic, Human Chorionic Gonadotropin Related Protein, Pregnancy",
author = "Lee, {Jae Kwan} and Oh, {Min Jeong} and Shin, {Joong Sik} and Lee, {Kyung Joo} and Nam, {Jung Hyun} and Cha, {Jung Hak} and Chang, {Jin Dong} and Cho, {Dong Hee} and Kang, {In Soo} and Lee, {Paul I.}",
year = "2005",
month = "6",
day = "1",
language = "English",
volume = "20",
pages = "461--467",
journal = "Journal of Korean Medical Science",
issn = "1011-8934",
publisher = "Korean Academy of Medical Science",
number = "3",

}

TY - JOUR

T1 - Clinical effectiveness of urinary human chorionic gonadotropin related protein (hCGRP) quantification for diagnosis of ectopic pregnancy

AU - Lee, Jae Kwan

AU - Oh, Min Jeong

AU - Shin, Joong Sik

AU - Lee, Kyung Joo

AU - Nam, Jung Hyun

AU - Cha, Jung Hak

AU - Chang, Jin Dong

AU - Cho, Dong Hee

AU - Kang, In Soo

AU - Lee, Paul I.

PY - 2005/6/1

Y1 - 2005/6/1

N2 - We detected pregnancy related new molecule, human chorionic gonadotropin related protein (hCGRP) in the urine of a pregnant women by using a monoclonal antibody against the human chorionic gonadotropin (hCG). This study examined the effectiveness of urinary hCGRP quantification in diagnosing ectopic pregnancy. This study included 40 normal pregnant women and 25 patients with ectopic pregnancy. Patients' serum and urinary intact whole hCG (i-hCG) and hCGRP concentrations were measured using sandwich ELISA and the ratio of hCGRP to i-hCG was calculated. Statistical analysis was performed using statistical package for social sciences (SPSS) 10.0. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the cut-off value to discriminate ectopic pregnancies from normal intrauterine pregnancies. Urinary hCGRP and hCGRP/i-hCG ratio in ectopic pregnancy group (14±6.6 ng/mL, 4.6±1.9%, respectively) were significantly lower than those of normal pregnancy group (149±10.2 ng/mL, 29.7±1.9%, respectively; p<0.001). Based on ROC curve analysis, a cut-off point of urinary hCGRP/i-hCG ratio <16.2% discriminated between ectopic pregnancy and normal pregnancy with a sensitivity, specificity, positive predictive value and negative predictive value of 92.0%, 90.0%, 32.6%, and 99.5%, respectively. Urinary hCGRP/i-hCG ratio measurement may be effective in diagnosing ectopic pregnancy.

AB - We detected pregnancy related new molecule, human chorionic gonadotropin related protein (hCGRP) in the urine of a pregnant women by using a monoclonal antibody against the human chorionic gonadotropin (hCG). This study examined the effectiveness of urinary hCGRP quantification in diagnosing ectopic pregnancy. This study included 40 normal pregnant women and 25 patients with ectopic pregnancy. Patients' serum and urinary intact whole hCG (i-hCG) and hCGRP concentrations were measured using sandwich ELISA and the ratio of hCGRP to i-hCG was calculated. Statistical analysis was performed using statistical package for social sciences (SPSS) 10.0. Receiver operating characteristic (ROC) curve analysis was performed to evaluate the cut-off value to discriminate ectopic pregnancies from normal intrauterine pregnancies. Urinary hCGRP and hCGRP/i-hCG ratio in ectopic pregnancy group (14±6.6 ng/mL, 4.6±1.9%, respectively) were significantly lower than those of normal pregnancy group (149±10.2 ng/mL, 29.7±1.9%, respectively; p<0.001). Based on ROC curve analysis, a cut-off point of urinary hCGRP/i-hCG ratio <16.2% discriminated between ectopic pregnancy and normal pregnancy with a sensitivity, specificity, positive predictive value and negative predictive value of 92.0%, 90.0%, 32.6%, and 99.5%, respectively. Urinary hCGRP/i-hCG ratio measurement may be effective in diagnosing ectopic pregnancy.

KW - Chorionic Gonadotropin

KW - Early Diagnosis

KW - Ectopic

KW - Human Chorionic Gonadotropin Related Protein

KW - Pregnancy

UR - http://www.scopus.com/inward/record.url?scp=21344454233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21344454233&partnerID=8YFLogxK

M3 - Article

C2 - 15953870

AN - SCOPUS:21344454233

VL - 20

SP - 461

EP - 467

JO - Journal of Korean Medical Science

JF - Journal of Korean Medical Science

SN - 1011-8934

IS - 3

ER -