Clinical performance assessment of five human papillomavirus DNA tests using liquid-based cytology samples

Seung Hun Song, Jin-Hwa Hong, San Ha Kwak, Jae Kwan Lee, Mi Kyung Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Aim: To evaluate the performance of five HPV tests (Hybrid Capture 2, Linear Array [LA], DNA chip, type-specific polymerase chain reaction [PCR], and sequencing) in detecting high-risk (HR) HPV DNA and high-grade cervical intraepithelial neoplasia (CIN) and cancer. Material and Methods: A total of 137 women with abnormal cytologies were prospectively enrolled. The diagnostic accuracy of five HPV DNA tests in detecting high-grade CIN and cancer was assessed, and the concordance among HPV genotyping tests was evaluated. All specimens were obtained from cervical swab samples preserved in residual PreservCyt solution that were originally collected for ThinPrep Pap test. Results: The sensitivities of all five HPV tests did not differ significantly among the five HPV tests. The overall concordance levelwas excellent between LA and sequencing (98.2%, Kappa = 0.9) andwas substantial between DNA chip and sequencing (96.4%, Kappa = 0.8). The concordance levels for detecting vaccine-type (HPV 16 and 18) of LA in comparison with DNA chip, type-specific PCR, and sequencing were substantial (Kappa = 0.67, 0.64, and 0.65, respectively). The sensitivities for all five HPV tests were >90% for the detection of CIN2 or higher. No statistically significant differences in sensitivity occurred between any of the tests. Conclusion: All five HPV tests showed reliable results in detecting HR HPVs, high-grade CIN, and cancer. Both LA and DNA chip tests may be useful in identifying HR HPV, especially when multiple genotypes are present.

Original languageEnglish
Pages (from-to)408-414
Number of pages7
JournalJournal of Obstetrics and Gynaecology Research
Volume38
Issue number2
DOIs
Publication statusPublished - 2012 Feb 1

Fingerprint

Human Papillomavirus DNA Tests
Oligonucleotide Array Sequence Analysis
Cervical Intraepithelial Neoplasia
Cell Biology
Papanicolaou Test
Human papillomavirus 18
Neoplasms
Polymerase Chain Reaction
Human papillomavirus 16
DNA Sequence Analysis
Vaccines
Genotype
DNA

Keywords

  • Cervical cancer
  • Cervical intraepithelial neoplasia
  • Genotyping
  • Human papillomavirus

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Clinical performance assessment of five human papillomavirus DNA tests using liquid-based cytology samples. / Song, Seung Hun; Hong, Jin-Hwa; Kwak, San Ha; Lee, Jae Kwan; Kim, Mi Kyung.

In: Journal of Obstetrics and Gynaecology Research, Vol. 38, No. 2, 01.02.2012, p. 408-414.

Research output: Contribution to journalArticle

@article{c61b5adf790b4c689c1059fcc38d335d,
title = "Clinical performance assessment of five human papillomavirus DNA tests using liquid-based cytology samples",
abstract = "Aim: To evaluate the performance of five HPV tests (Hybrid Capture 2, Linear Array [LA], DNA chip, type-specific polymerase chain reaction [PCR], and sequencing) in detecting high-risk (HR) HPV DNA and high-grade cervical intraepithelial neoplasia (CIN) and cancer. Material and Methods: A total of 137 women with abnormal cytologies were prospectively enrolled. The diagnostic accuracy of five HPV DNA tests in detecting high-grade CIN and cancer was assessed, and the concordance among HPV genotyping tests was evaluated. All specimens were obtained from cervical swab samples preserved in residual PreservCyt solution that were originally collected for ThinPrep Pap test. Results: The sensitivities of all five HPV tests did not differ significantly among the five HPV tests. The overall concordance levelwas excellent between LA and sequencing (98.2{\%}, Kappa = 0.9) andwas substantial between DNA chip and sequencing (96.4{\%}, Kappa = 0.8). The concordance levels for detecting vaccine-type (HPV 16 and 18) of LA in comparison with DNA chip, type-specific PCR, and sequencing were substantial (Kappa = 0.67, 0.64, and 0.65, respectively). The sensitivities for all five HPV tests were >90{\%} for the detection of CIN2 or higher. No statistically significant differences in sensitivity occurred between any of the tests. Conclusion: All five HPV tests showed reliable results in detecting HR HPVs, high-grade CIN, and cancer. Both LA and DNA chip tests may be useful in identifying HR HPV, especially when multiple genotypes are present.",
keywords = "Cervical cancer, Cervical intraepithelial neoplasia, Genotyping, Human papillomavirus",
author = "Song, {Seung Hun} and Jin-Hwa Hong and Kwak, {San Ha} and Lee, {Jae Kwan} and Kim, {Mi Kyung}",
year = "2012",
month = "2",
day = "1",
doi = "10.1111/j.1447-0756.2011.01709.x",
language = "English",
volume = "38",
pages = "408--414",
journal = "Journal of Obstetrics and Gynaecology Research",
issn = "1341-8076",
publisher = "Blackwell Publishing Asia",
number = "2",

}

TY - JOUR

T1 - Clinical performance assessment of five human papillomavirus DNA tests using liquid-based cytology samples

AU - Song, Seung Hun

AU - Hong, Jin-Hwa

AU - Kwak, San Ha

AU - Lee, Jae Kwan

AU - Kim, Mi Kyung

PY - 2012/2/1

Y1 - 2012/2/1

N2 - Aim: To evaluate the performance of five HPV tests (Hybrid Capture 2, Linear Array [LA], DNA chip, type-specific polymerase chain reaction [PCR], and sequencing) in detecting high-risk (HR) HPV DNA and high-grade cervical intraepithelial neoplasia (CIN) and cancer. Material and Methods: A total of 137 women with abnormal cytologies were prospectively enrolled. The diagnostic accuracy of five HPV DNA tests in detecting high-grade CIN and cancer was assessed, and the concordance among HPV genotyping tests was evaluated. All specimens were obtained from cervical swab samples preserved in residual PreservCyt solution that were originally collected for ThinPrep Pap test. Results: The sensitivities of all five HPV tests did not differ significantly among the five HPV tests. The overall concordance levelwas excellent between LA and sequencing (98.2%, Kappa = 0.9) andwas substantial between DNA chip and sequencing (96.4%, Kappa = 0.8). The concordance levels for detecting vaccine-type (HPV 16 and 18) of LA in comparison with DNA chip, type-specific PCR, and sequencing were substantial (Kappa = 0.67, 0.64, and 0.65, respectively). The sensitivities for all five HPV tests were >90% for the detection of CIN2 or higher. No statistically significant differences in sensitivity occurred between any of the tests. Conclusion: All five HPV tests showed reliable results in detecting HR HPVs, high-grade CIN, and cancer. Both LA and DNA chip tests may be useful in identifying HR HPV, especially when multiple genotypes are present.

AB - Aim: To evaluate the performance of five HPV tests (Hybrid Capture 2, Linear Array [LA], DNA chip, type-specific polymerase chain reaction [PCR], and sequencing) in detecting high-risk (HR) HPV DNA and high-grade cervical intraepithelial neoplasia (CIN) and cancer. Material and Methods: A total of 137 women with abnormal cytologies were prospectively enrolled. The diagnostic accuracy of five HPV DNA tests in detecting high-grade CIN and cancer was assessed, and the concordance among HPV genotyping tests was evaluated. All specimens were obtained from cervical swab samples preserved in residual PreservCyt solution that were originally collected for ThinPrep Pap test. Results: The sensitivities of all five HPV tests did not differ significantly among the five HPV tests. The overall concordance levelwas excellent between LA and sequencing (98.2%, Kappa = 0.9) andwas substantial between DNA chip and sequencing (96.4%, Kappa = 0.8). The concordance levels for detecting vaccine-type (HPV 16 and 18) of LA in comparison with DNA chip, type-specific PCR, and sequencing were substantial (Kappa = 0.67, 0.64, and 0.65, respectively). The sensitivities for all five HPV tests were >90% for the detection of CIN2 or higher. No statistically significant differences in sensitivity occurred between any of the tests. Conclusion: All five HPV tests showed reliable results in detecting HR HPVs, high-grade CIN, and cancer. Both LA and DNA chip tests may be useful in identifying HR HPV, especially when multiple genotypes are present.

KW - Cervical cancer

KW - Cervical intraepithelial neoplasia

KW - Genotyping

KW - Human papillomavirus

UR - http://www.scopus.com/inward/record.url?scp=84860847861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860847861&partnerID=8YFLogxK

U2 - 10.1111/j.1447-0756.2011.01709.x

DO - 10.1111/j.1447-0756.2011.01709.x

M3 - Article

C2 - 22175246

AN - SCOPUS:84860847861

VL - 38

SP - 408

EP - 414

JO - Journal of Obstetrics and Gynaecology Research

JF - Journal of Obstetrics and Gynaecology Research

SN - 1341-8076

IS - 2

ER -