Abstract
Early identification of influenza is important for optimal patient management and infection control. Rapid influenza antigen tests have been used routinely in clinical settings to confirm clinical suspicion, despite their low sensitivity. To improve sensitivity, various influenza point-of-care test reader systems have been developed. This study evaluated the clinical performance of a digital readout rapid influenza diagnostic test (RIDT), the BD Veritor™ System Flu A+B assay (BD). Nasopharyngeal swabs taken from 250 patients (influenza A positive, n=75; influenza B positive, n=75; and influenza negative, n=100) were analyzed using the BinaxNOW® Influenza A/B antigen kit (BN), SD Influenza Ag A/B kit (SD), BD, real-time reverse transcriptase polymerase chain reaction (RT-PCR), and an influenza virus culture. Compared to RT-PCR, the sensitivities of BN, SD, and BD were 56.0, 53.3, and 72.0%, respectively, for influenza A and 57.3, 65.3, and 69.3%, respectively, for influenza B. No false-positive results were noted with the three rapid antigen tests. For influenza A, the average RT-PCR threshold cycle (Ct) for specimens that tested positive using BD was higher than that for specimens that tested positive using BN and SD. BD is a sensitive and easy method for the early detection of influenza A and B.
| Original language | English |
|---|---|
| Pages (from-to) | 86-90 |
| Number of pages | 5 |
| Journal | Journal of Virological Methods |
| Volume | 204 |
| DOIs | |
| Publication status | Published - 2014 Jan 1 |
Fingerprint
Keywords
- BD Veritor™ System
- Evaluation
- Influenza
- Point-of-care
- Rapid antigen test
- Sensitivity
ASJC Scopus subject areas
- Virology
Cite this
Clinical performance evaluation of the BD Veritor System Flu A + B assay. / Nam, Myung-Hyun; Jang, Jin Woo; Lee, Jong Han; Cho, Chy Hyun; Lim, Chae Seung; Kim, Woo Joo.
In: Journal of Virological Methods, Vol. 204, 01.01.2014, p. 86-90.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clinical performance evaluation of the BD Veritor System Flu A + B assay
AU - Nam, Myung-Hyun
AU - Jang, Jin Woo
AU - Lee, Jong Han
AU - Cho, Chy Hyun
AU - Lim, Chae Seung
AU - Kim, Woo Joo
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Early identification of influenza is important for optimal patient management and infection control. Rapid influenza antigen tests have been used routinely in clinical settings to confirm clinical suspicion, despite their low sensitivity. To improve sensitivity, various influenza point-of-care test reader systems have been developed. This study evaluated the clinical performance of a digital readout rapid influenza diagnostic test (RIDT), the BD Veritor™ System Flu A+B assay (BD). Nasopharyngeal swabs taken from 250 patients (influenza A positive, n=75; influenza B positive, n=75; and influenza negative, n=100) were analyzed using the BinaxNOW® Influenza A/B antigen kit (BN), SD Influenza Ag A/B kit (SD), BD, real-time reverse transcriptase polymerase chain reaction (RT-PCR), and an influenza virus culture. Compared to RT-PCR, the sensitivities of BN, SD, and BD were 56.0, 53.3, and 72.0%, respectively, for influenza A and 57.3, 65.3, and 69.3%, respectively, for influenza B. No false-positive results were noted with the three rapid antigen tests. For influenza A, the average RT-PCR threshold cycle (Ct) for specimens that tested positive using BD was higher than that for specimens that tested positive using BN and SD. BD is a sensitive and easy method for the early detection of influenza A and B.
AB - Early identification of influenza is important for optimal patient management and infection control. Rapid influenza antigen tests have been used routinely in clinical settings to confirm clinical suspicion, despite their low sensitivity. To improve sensitivity, various influenza point-of-care test reader systems have been developed. This study evaluated the clinical performance of a digital readout rapid influenza diagnostic test (RIDT), the BD Veritor™ System Flu A+B assay (BD). Nasopharyngeal swabs taken from 250 patients (influenza A positive, n=75; influenza B positive, n=75; and influenza negative, n=100) were analyzed using the BinaxNOW® Influenza A/B antigen kit (BN), SD Influenza Ag A/B kit (SD), BD, real-time reverse transcriptase polymerase chain reaction (RT-PCR), and an influenza virus culture. Compared to RT-PCR, the sensitivities of BN, SD, and BD were 56.0, 53.3, and 72.0%, respectively, for influenza A and 57.3, 65.3, and 69.3%, respectively, for influenza B. No false-positive results were noted with the three rapid antigen tests. For influenza A, the average RT-PCR threshold cycle (Ct) for specimens that tested positive using BD was higher than that for specimens that tested positive using BN and SD. BD is a sensitive and easy method for the early detection of influenza A and B.
KW - BD Veritor™ System
KW - Evaluation
KW - Influenza
KW - Point-of-care
KW - Rapid antigen test
KW - Sensitivity
UR - http://www.scopus.com/inward/record.url?scp=84899840895&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84899840895&partnerID=8YFLogxK
U2 - 10.1016/j.jviromet.2014.04.009
DO - 10.1016/j.jviromet.2014.04.009
M3 - Article
VL - 204
SP - 86
EP - 90
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
ER -