Abstract
Objective: TheAllplexTM Respiratory Panel 1 (ARP) is a new assay based on a real-time polymerase chain reaction (RT-PCR) for the detection of influenza A (Flu A), influenza B virus (Flu B), and respiratory syncytial virus (RSV), including subtyping by multiple detection temperature (MuDT) technology. We evaluated the performance of the Allplex Respiratory Panel compared to the SimplexaTM Flu A/B & RSV assay (SP) and other diagnostic tools. Materials and Methods: A total of 372 samples were collected from patients at the Korea University Guro Hospital in Seoul, Korea. All samples were tested for influenza virus and RSV by ARP, SP, and an in-house RT-PCR. Results: The sensitivity of ARP was 95.56, 100, and 95.24% for Flu A, Flu B, and RSV, respectively. The specificity of ARP was 100, 100, and 100% for Flu A, Flu B, and RSV, respectively. SP had sensitivities and specificities of 98.89 and 100% for Flu A, 100 and 100% for Flu B, and 100 and 100% for RSV. Conclusion: The Allplex panelshowed high sensitivity, specificity, positive predictive, and negative predictive values for the detection of Flu A, Flu B, and RSV. This assay is fast and easy to perform because it takes only about 150 min and there is no need for post-PCR electrophoresis. The ARP can be used as a reliable and convenient assay in clinical laboratories.
| Original language | English |
|---|---|
| Pages (from-to) | 380-386 |
| Number of pages | 7 |
| Journal | Medical Principles and Practice |
| Volume | 28 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2019 Jul 1 |
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Keywords
- Allplex Respiratory Panel 1
- Influenza
- Real-time polymerase chain reaction
- Respiratory syncytial virus
- Respiratory virus
ASJC Scopus subject areas
- Medicine(all)
Cite this
Clinical Performance of the AllplexTM Respiratory Panel 1 Test Compared to SimplexaTM Flu A/B and RSV for Detection of Influenza Virus and Respiratory Syncytial Virus Infection Including Their Subtyping. / Kim, Jeeyong; Nam, Jeonghun; Jang, Woongsik; Lim, Chae Seung.
In: Medical Principles and Practice, Vol. 28, No. 4, 01.07.2019, p. 380-386.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clinical Performance of the AllplexTM Respiratory Panel 1 Test Compared to SimplexaTM Flu A/B and RSV for Detection of Influenza Virus and Respiratory Syncytial Virus Infection Including Their Subtyping
AU - Kim, Jeeyong
AU - Nam, Jeonghun
AU - Jang, Woongsik
AU - Lim, Chae Seung
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Objective: TheAllplexTM Respiratory Panel 1 (ARP) is a new assay based on a real-time polymerase chain reaction (RT-PCR) for the detection of influenza A (Flu A), influenza B virus (Flu B), and respiratory syncytial virus (RSV), including subtyping by multiple detection temperature (MuDT) technology. We evaluated the performance of the Allplex Respiratory Panel compared to the SimplexaTM Flu A/B & RSV assay (SP) and other diagnostic tools. Materials and Methods: A total of 372 samples were collected from patients at the Korea University Guro Hospital in Seoul, Korea. All samples were tested for influenza virus and RSV by ARP, SP, and an in-house RT-PCR. Results: The sensitivity of ARP was 95.56, 100, and 95.24% for Flu A, Flu B, and RSV, respectively. The specificity of ARP was 100, 100, and 100% for Flu A, Flu B, and RSV, respectively. SP had sensitivities and specificities of 98.89 and 100% for Flu A, 100 and 100% for Flu B, and 100 and 100% for RSV. Conclusion: The Allplex panelshowed high sensitivity, specificity, positive predictive, and negative predictive values for the detection of Flu A, Flu B, and RSV. This assay is fast and easy to perform because it takes only about 150 min and there is no need for post-PCR electrophoresis. The ARP can be used as a reliable and convenient assay in clinical laboratories.
AB - Objective: TheAllplexTM Respiratory Panel 1 (ARP) is a new assay based on a real-time polymerase chain reaction (RT-PCR) for the detection of influenza A (Flu A), influenza B virus (Flu B), and respiratory syncytial virus (RSV), including subtyping by multiple detection temperature (MuDT) technology. We evaluated the performance of the Allplex Respiratory Panel compared to the SimplexaTM Flu A/B & RSV assay (SP) and other diagnostic tools. Materials and Methods: A total of 372 samples were collected from patients at the Korea University Guro Hospital in Seoul, Korea. All samples were tested for influenza virus and RSV by ARP, SP, and an in-house RT-PCR. Results: The sensitivity of ARP was 95.56, 100, and 95.24% for Flu A, Flu B, and RSV, respectively. The specificity of ARP was 100, 100, and 100% for Flu A, Flu B, and RSV, respectively. SP had sensitivities and specificities of 98.89 and 100% for Flu A, 100 and 100% for Flu B, and 100 and 100% for RSV. Conclusion: The Allplex panelshowed high sensitivity, specificity, positive predictive, and negative predictive values for the detection of Flu A, Flu B, and RSV. This assay is fast and easy to perform because it takes only about 150 min and there is no need for post-PCR electrophoresis. The ARP can be used as a reliable and convenient assay in clinical laboratories.
KW - Allplex Respiratory Panel 1
KW - Influenza
KW - Real-time polymerase chain reaction
KW - Respiratory syncytial virus
KW - Respiratory virus
UR - http://www.scopus.com/inward/record.url?scp=85069540758&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85069540758&partnerID=8YFLogxK
U2 - 10.1159/000499313
DO - 10.1159/000499313
M3 - Article
C2 - 30831570
AN - SCOPUS:85069540758
VL - 28
SP - 380
EP - 386
JO - Medical Principles and Practice
JF - Medical Principles and Practice
SN - 1011-7571
IS - 4
ER -