Clonal cell populations unresponsive to radiosensitization induced by telomerase inhibition

Yeun Jin Ju, Hyun Jin Shin, Jeong Eun Park, Kyoung Mi Juhn, Seon Rang Woo, Hee Young Kim, Young Hoon Han, Sang Gu Hwang, Sung Hee Hong, Chang Mo Kang, Young Do Yoo, Won Bong Park, Myung Haing Cho, Gil-Hong Park, Kee Ho Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A combination of a radiotherapeutic regimen with telomerase inhibition is valuable when tumor cells are to be sensitized to radiation. Here, we describe cell clones unresponsive to radiosensitization after telomere shortening. After extensive division of individual transformed clones of mTERC-/- cells, about 22% of clones were unresponsive to radiosensitization even though telomerase action was inhibited. The telomere lengths of unsensitized mTERC-/- clones were reduced, as were those of most sensitized clones. However, the unsensitized clones did not exhibit chromosomal end-to-end fusion to the extent noted in all sensitized clones. Thus, a defense mechanism preventing telomere erosion is operative even when telomeres become shorter under conditions of telomerase deficiency, and results in unresponsiveness to the radiosensitization generally mediated by telomere shortening.

Original languageEnglish
Pages (from-to)198-202
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume402
Issue number2
DOIs
Publication statusPublished - 2010 Nov 12

Fingerprint

Telomerase
Clone Cells
Cells
Clone cells
Telomere
Population
Telomere Shortening
Tumors
Erosion
Fusion reactions
Radiation
Neoplasms

Keywords

  • Chromosomal end-to-end fusion
  • Radiosensitization
  • Telomere
  • Telomere length

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Clonal cell populations unresponsive to radiosensitization induced by telomerase inhibition. / Ju, Yeun Jin; Shin, Hyun Jin; Park, Jeong Eun; Juhn, Kyoung Mi; Woo, Seon Rang; Kim, Hee Young; Han, Young Hoon; Hwang, Sang Gu; Hong, Sung Hee; Kang, Chang Mo; Yoo, Young Do; Park, Won Bong; Cho, Myung Haing; Park, Gil-Hong; Lee, Kee Ho.

In: Biochemical and Biophysical Research Communications, Vol. 402, No. 2, 12.11.2010, p. 198-202.

Research output: Contribution to journalArticle

Ju, YJ, Shin, HJ, Park, JE, Juhn, KM, Woo, SR, Kim, HY, Han, YH, Hwang, SG, Hong, SH, Kang, CM, Yoo, YD, Park, WB, Cho, MH, Park, G-H & Lee, KH 2010, 'Clonal cell populations unresponsive to radiosensitization induced by telomerase inhibition', Biochemical and Biophysical Research Communications, vol. 402, no. 2, pp. 198-202. https://doi.org/10.1016/j.bbrc.2010.09.091
Ju, Yeun Jin ; Shin, Hyun Jin ; Park, Jeong Eun ; Juhn, Kyoung Mi ; Woo, Seon Rang ; Kim, Hee Young ; Han, Young Hoon ; Hwang, Sang Gu ; Hong, Sung Hee ; Kang, Chang Mo ; Yoo, Young Do ; Park, Won Bong ; Cho, Myung Haing ; Park, Gil-Hong ; Lee, Kee Ho. / Clonal cell populations unresponsive to radiosensitization induced by telomerase inhibition. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 402, No. 2. pp. 198-202.
@article{ac0a2e3566774e63b92925fb393b301a,
title = "Clonal cell populations unresponsive to radiosensitization induced by telomerase inhibition",
abstract = "A combination of a radiotherapeutic regimen with telomerase inhibition is valuable when tumor cells are to be sensitized to radiation. Here, we describe cell clones unresponsive to radiosensitization after telomere shortening. After extensive division of individual transformed clones of mTERC-/- cells, about 22{\%} of clones were unresponsive to radiosensitization even though telomerase action was inhibited. The telomere lengths of unsensitized mTERC-/- clones were reduced, as were those of most sensitized clones. However, the unsensitized clones did not exhibit chromosomal end-to-end fusion to the extent noted in all sensitized clones. Thus, a defense mechanism preventing telomere erosion is operative even when telomeres become shorter under conditions of telomerase deficiency, and results in unresponsiveness to the radiosensitization generally mediated by telomere shortening.",
keywords = "Chromosomal end-to-end fusion, Radiosensitization, Telomere, Telomere length",
author = "Ju, {Yeun Jin} and Shin, {Hyun Jin} and Park, {Jeong Eun} and Juhn, {Kyoung Mi} and Woo, {Seon Rang} and Kim, {Hee Young} and Han, {Young Hoon} and Hwang, {Sang Gu} and Hong, {Sung Hee} and Kang, {Chang Mo} and Yoo, {Young Do} and Park, {Won Bong} and Cho, {Myung Haing} and Gil-Hong Park and Lee, {Kee Ho}",
year = "2010",
month = "11",
day = "12",
doi = "10.1016/j.bbrc.2010.09.091",
language = "English",
volume = "402",
pages = "198--202",
journal = "The BMJ",
issn = "0730-6512",
publisher = "Kluwer Academic Publishers",
number = "2",

}

TY - JOUR

T1 - Clonal cell populations unresponsive to radiosensitization induced by telomerase inhibition

AU - Ju, Yeun Jin

AU - Shin, Hyun Jin

AU - Park, Jeong Eun

AU - Juhn, Kyoung Mi

AU - Woo, Seon Rang

AU - Kim, Hee Young

AU - Han, Young Hoon

AU - Hwang, Sang Gu

AU - Hong, Sung Hee

AU - Kang, Chang Mo

AU - Yoo, Young Do

AU - Park, Won Bong

AU - Cho, Myung Haing

AU - Park, Gil-Hong

AU - Lee, Kee Ho

PY - 2010/11/12

Y1 - 2010/11/12

N2 - A combination of a radiotherapeutic regimen with telomerase inhibition is valuable when tumor cells are to be sensitized to radiation. Here, we describe cell clones unresponsive to radiosensitization after telomere shortening. After extensive division of individual transformed clones of mTERC-/- cells, about 22% of clones were unresponsive to radiosensitization even though telomerase action was inhibited. The telomere lengths of unsensitized mTERC-/- clones were reduced, as were those of most sensitized clones. However, the unsensitized clones did not exhibit chromosomal end-to-end fusion to the extent noted in all sensitized clones. Thus, a defense mechanism preventing telomere erosion is operative even when telomeres become shorter under conditions of telomerase deficiency, and results in unresponsiveness to the radiosensitization generally mediated by telomere shortening.

AB - A combination of a radiotherapeutic regimen with telomerase inhibition is valuable when tumor cells are to be sensitized to radiation. Here, we describe cell clones unresponsive to radiosensitization after telomere shortening. After extensive division of individual transformed clones of mTERC-/- cells, about 22% of clones were unresponsive to radiosensitization even though telomerase action was inhibited. The telomere lengths of unsensitized mTERC-/- clones were reduced, as were those of most sensitized clones. However, the unsensitized clones did not exhibit chromosomal end-to-end fusion to the extent noted in all sensitized clones. Thus, a defense mechanism preventing telomere erosion is operative even when telomeres become shorter under conditions of telomerase deficiency, and results in unresponsiveness to the radiosensitization generally mediated by telomere shortening.

KW - Chromosomal end-to-end fusion

KW - Radiosensitization

KW - Telomere

KW - Telomere length

UR - http://www.scopus.com/inward/record.url?scp=78149470396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78149470396&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2010.09.091

DO - 10.1016/j.bbrc.2010.09.091

M3 - Article

VL - 402

SP - 198

EP - 202

JO - The BMJ

JF - The BMJ

SN - 0730-6512

IS - 2

ER -