Cloning and Analysis of the Epstein-Barr Virus Glycoprotein 350 Genes

Sun Hwa Chang, Seon Hee Kim, Won Keun Lee, Hyun Ju Kim, Sang Hoon Choi, Joo Hung Park, Hyon Seok Jang, Gook Hyun Chung, Tae Ho Kwon, Dae Hyuk Kim, Moon Sik Yang, Yong Suk Jang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Membrane glycoprotein 350 (gp350) of the Epstein-Barr virus (EBV) is considered as a major target for vaccine development, since the gp350 has been identified as the virus' mediator for receptor interaction and as an inducer of specific in vitro virus-neutralizing antibodies. In an initial attempt to develop an effective DNA vaccine against an EBV infection, gp350 genes were isolated from SNU-20 and SNU-1103 which are the EBV-infected lymphoblastoid cell lines established in Korea. In addition, the nucleotide sequences of the gp350 genes were determined and compared with those of other EBV strains such as B95-8, P3HR-1/AG876 and M81. Sequence analysis showed that similar high degrees of homology between 2 EBV strains derived from African Burkitt's lymphoma, P3HR-1 and AG876, was shown between the gp350 genes isolated from 2 EBV-infected lymphoblastoid cell lines established in Korea. Furthermore, these 2 Korean and 2 African strains displayed nearly identical patterns of sequence variations from B95-8. In addition, the sequence of the isolated gp350 genes, which have been reported to be associated with the biology of EBV infection, is analyzed.

Original languageEnglish
Pages (from-to)585-593
Number of pages9
JournalMolecules and Cells
Volume8
Issue number5
Publication statusPublished - 1998 Oct 31

Fingerprint

Human Herpesvirus 4
Organism Cloning
Glycoproteins
Epstein-Barr Virus Infections
Burkitt Lymphoma
Genes
Korea
Virus Receptors
Cell Line
DNA Vaccines
Membrane Glycoproteins
Neutralizing Antibodies
Sequence Analysis
Vaccines
Viruses

Keywords

  • Epstein-Barr Virus
  • Glycoprotein 350
  • Vaccine

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Chang, S. H., Kim, S. H., Lee, W. K., Kim, H. J., Choi, S. H., Park, J. H., ... Jang, Y. S. (1998). Cloning and Analysis of the Epstein-Barr Virus Glycoprotein 350 Genes. Molecules and Cells, 8(5), 585-593.

Cloning and Analysis of the Epstein-Barr Virus Glycoprotein 350 Genes. / Chang, Sun Hwa; Kim, Seon Hee; Lee, Won Keun; Kim, Hyun Ju; Choi, Sang Hoon; Park, Joo Hung; Jang, Hyon Seok; Chung, Gook Hyun; Kwon, Tae Ho; Kim, Dae Hyuk; Yang, Moon Sik; Jang, Yong Suk.

In: Molecules and Cells, Vol. 8, No. 5, 31.10.1998, p. 585-593.

Research output: Contribution to journalArticle

Chang, SH, Kim, SH, Lee, WK, Kim, HJ, Choi, SH, Park, JH, Jang, HS, Chung, GH, Kwon, TH, Kim, DH, Yang, MS & Jang, YS 1998, 'Cloning and Analysis of the Epstein-Barr Virus Glycoprotein 350 Genes', Molecules and Cells, vol. 8, no. 5, pp. 585-593.
Chang SH, Kim SH, Lee WK, Kim HJ, Choi SH, Park JH et al. Cloning and Analysis of the Epstein-Barr Virus Glycoprotein 350 Genes. Molecules and Cells. 1998 Oct 31;8(5):585-593.
Chang, Sun Hwa ; Kim, Seon Hee ; Lee, Won Keun ; Kim, Hyun Ju ; Choi, Sang Hoon ; Park, Joo Hung ; Jang, Hyon Seok ; Chung, Gook Hyun ; Kwon, Tae Ho ; Kim, Dae Hyuk ; Yang, Moon Sik ; Jang, Yong Suk. / Cloning and Analysis of the Epstein-Barr Virus Glycoprotein 350 Genes. In: Molecules and Cells. 1998 ; Vol. 8, No. 5. pp. 585-593.
@article{26e01d67adac4e7b8d783ba74a7379ee,
title = "Cloning and Analysis of the Epstein-Barr Virus Glycoprotein 350 Genes",
abstract = "Membrane glycoprotein 350 (gp350) of the Epstein-Barr virus (EBV) is considered as a major target for vaccine development, since the gp350 has been identified as the virus' mediator for receptor interaction and as an inducer of specific in vitro virus-neutralizing antibodies. In an initial attempt to develop an effective DNA vaccine against an EBV infection, gp350 genes were isolated from SNU-20 and SNU-1103 which are the EBV-infected lymphoblastoid cell lines established in Korea. In addition, the nucleotide sequences of the gp350 genes were determined and compared with those of other EBV strains such as B95-8, P3HR-1/AG876 and M81. Sequence analysis showed that similar high degrees of homology between 2 EBV strains derived from African Burkitt's lymphoma, P3HR-1 and AG876, was shown between the gp350 genes isolated from 2 EBV-infected lymphoblastoid cell lines established in Korea. Furthermore, these 2 Korean and 2 African strains displayed nearly identical patterns of sequence variations from B95-8. In addition, the sequence of the isolated gp350 genes, which have been reported to be associated with the biology of EBV infection, is analyzed.",
keywords = "Epstein-Barr Virus, Glycoprotein 350, Vaccine",
author = "Chang, {Sun Hwa} and Kim, {Seon Hee} and Lee, {Won Keun} and Kim, {Hyun Ju} and Choi, {Sang Hoon} and Park, {Joo Hung} and Jang, {Hyon Seok} and Chung, {Gook Hyun} and Kwon, {Tae Ho} and Kim, {Dae Hyuk} and Yang, {Moon Sik} and Jang, {Yong Suk}",
year = "1998",
month = "10",
day = "31",
language = "English",
volume = "8",
pages = "585--593",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "5",

}

TY - JOUR

T1 - Cloning and Analysis of the Epstein-Barr Virus Glycoprotein 350 Genes

AU - Chang, Sun Hwa

AU - Kim, Seon Hee

AU - Lee, Won Keun

AU - Kim, Hyun Ju

AU - Choi, Sang Hoon

AU - Park, Joo Hung

AU - Jang, Hyon Seok

AU - Chung, Gook Hyun

AU - Kwon, Tae Ho

AU - Kim, Dae Hyuk

AU - Yang, Moon Sik

AU - Jang, Yong Suk

PY - 1998/10/31

Y1 - 1998/10/31

N2 - Membrane glycoprotein 350 (gp350) of the Epstein-Barr virus (EBV) is considered as a major target for vaccine development, since the gp350 has been identified as the virus' mediator for receptor interaction and as an inducer of specific in vitro virus-neutralizing antibodies. In an initial attempt to develop an effective DNA vaccine against an EBV infection, gp350 genes were isolated from SNU-20 and SNU-1103 which are the EBV-infected lymphoblastoid cell lines established in Korea. In addition, the nucleotide sequences of the gp350 genes were determined and compared with those of other EBV strains such as B95-8, P3HR-1/AG876 and M81. Sequence analysis showed that similar high degrees of homology between 2 EBV strains derived from African Burkitt's lymphoma, P3HR-1 and AG876, was shown between the gp350 genes isolated from 2 EBV-infected lymphoblastoid cell lines established in Korea. Furthermore, these 2 Korean and 2 African strains displayed nearly identical patterns of sequence variations from B95-8. In addition, the sequence of the isolated gp350 genes, which have been reported to be associated with the biology of EBV infection, is analyzed.

AB - Membrane glycoprotein 350 (gp350) of the Epstein-Barr virus (EBV) is considered as a major target for vaccine development, since the gp350 has been identified as the virus' mediator for receptor interaction and as an inducer of specific in vitro virus-neutralizing antibodies. In an initial attempt to develop an effective DNA vaccine against an EBV infection, gp350 genes were isolated from SNU-20 and SNU-1103 which are the EBV-infected lymphoblastoid cell lines established in Korea. In addition, the nucleotide sequences of the gp350 genes were determined and compared with those of other EBV strains such as B95-8, P3HR-1/AG876 and M81. Sequence analysis showed that similar high degrees of homology between 2 EBV strains derived from African Burkitt's lymphoma, P3HR-1 and AG876, was shown between the gp350 genes isolated from 2 EBV-infected lymphoblastoid cell lines established in Korea. Furthermore, these 2 Korean and 2 African strains displayed nearly identical patterns of sequence variations from B95-8. In addition, the sequence of the isolated gp350 genes, which have been reported to be associated with the biology of EBV infection, is analyzed.

KW - Epstein-Barr Virus

KW - Glycoprotein 350

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=0032585073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032585073&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 585

EP - 593

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 5

ER -