Cloning and characterization of androgen receptor from bullfrog, Rana catesbeiana

Soma Chattopadhyay, Jin Hee Park, Jae Young Seong, Hyuk Bang Kwon, Keesook Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have cloned and characterized a full-length cDNA of androgen receptor (AR) from the testis of bullfrog, Rana catesbeiana. The cDNA contains an open reading frame of 2328 nucleotides encoding a protein of 776 amino acid residues. The bullfrog AR shows high homology with ARs from other species in its amino acid sequence. Its overall homology with those of African clawed frog, Japanese eel and human is 70, 53, and 63%, respectively. As expected, the N-terminal domain shows much less homology (30-59%) than both DNA-binding domain (85-92%) and ligand binding domain (80-89%). Northern blot analysis detected the bullfrog AR message as a single transcript of around 9kb only in the testis. However, RT-PCR analysis revealed that AR mRNA is also expressed in other tissues although the levels are very low compared to that in the testis. Western blot analysis of whole tissue extracts showed the presence of AR protein in fore brain, heart, and testis. The AR gene is present as a single copy in bullfrog based on Southern blot analysis of genomic DNA. Altogether, the results suggest that the bullfrog AR is evolutionary conserved and may have functions similar to those shown in other species.

Original languageEnglish
Pages (from-to)10-17
Number of pages8
JournalGeneral and Comparative Endocrinology
Volume134
Issue number1
DOIs
Publication statusPublished - 2003 Oct 15
Externally publishedYes

Fingerprint

Rana catesbeiana
Androgen Receptors
Organism Cloning
Testis
Complementary DNA
Eels
Tissue Extracts
DNA
Southern Blotting
Northern Blotting
Anura
Open Reading Frames
Amino Acid Sequence
Proteins
Nucleotides
Western Blotting
Ligands
Amino Acids
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Androgen receptor
  • Bullfrog
  • cDNA

ASJC Scopus subject areas

  • Endocrinology

Cite this

Cloning and characterization of androgen receptor from bullfrog, Rana catesbeiana. / Chattopadhyay, Soma; Park, Jin Hee; Seong, Jae Young; Kwon, Hyuk Bang; Lee, Keesook.

In: General and Comparative Endocrinology, Vol. 134, No. 1, 15.10.2003, p. 10-17.

Research output: Contribution to journalArticle

Chattopadhyay, Soma ; Park, Jin Hee ; Seong, Jae Young ; Kwon, Hyuk Bang ; Lee, Keesook. / Cloning and characterization of androgen receptor from bullfrog, Rana catesbeiana. In: General and Comparative Endocrinology. 2003 ; Vol. 134, No. 1. pp. 10-17.
@article{39a4e9a3321049e8bd3403d91d0823f9,
title = "Cloning and characterization of androgen receptor from bullfrog, Rana catesbeiana",
abstract = "We have cloned and characterized a full-length cDNA of androgen receptor (AR) from the testis of bullfrog, Rana catesbeiana. The cDNA contains an open reading frame of 2328 nucleotides encoding a protein of 776 amino acid residues. The bullfrog AR shows high homology with ARs from other species in its amino acid sequence. Its overall homology with those of African clawed frog, Japanese eel and human is 70, 53, and 63{\%}, respectively. As expected, the N-terminal domain shows much less homology (30-59{\%}) than both DNA-binding domain (85-92{\%}) and ligand binding domain (80-89{\%}). Northern blot analysis detected the bullfrog AR message as a single transcript of around 9kb only in the testis. However, RT-PCR analysis revealed that AR mRNA is also expressed in other tissues although the levels are very low compared to that in the testis. Western blot analysis of whole tissue extracts showed the presence of AR protein in fore brain, heart, and testis. The AR gene is present as a single copy in bullfrog based on Southern blot analysis of genomic DNA. Altogether, the results suggest that the bullfrog AR is evolutionary conserved and may have functions similar to those shown in other species.",
keywords = "Androgen receptor, Bullfrog, cDNA",
author = "Soma Chattopadhyay and Park, {Jin Hee} and Seong, {Jae Young} and Kwon, {Hyuk Bang} and Keesook Lee",
year = "2003",
month = "10",
day = "15",
doi = "10.1016/S0016-6480(03)00215-6",
language = "English",
volume = "134",
pages = "10--17",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Cloning and characterization of androgen receptor from bullfrog, Rana catesbeiana

AU - Chattopadhyay, Soma

AU - Park, Jin Hee

AU - Seong, Jae Young

AU - Kwon, Hyuk Bang

AU - Lee, Keesook

PY - 2003/10/15

Y1 - 2003/10/15

N2 - We have cloned and characterized a full-length cDNA of androgen receptor (AR) from the testis of bullfrog, Rana catesbeiana. The cDNA contains an open reading frame of 2328 nucleotides encoding a protein of 776 amino acid residues. The bullfrog AR shows high homology with ARs from other species in its amino acid sequence. Its overall homology with those of African clawed frog, Japanese eel and human is 70, 53, and 63%, respectively. As expected, the N-terminal domain shows much less homology (30-59%) than both DNA-binding domain (85-92%) and ligand binding domain (80-89%). Northern blot analysis detected the bullfrog AR message as a single transcript of around 9kb only in the testis. However, RT-PCR analysis revealed that AR mRNA is also expressed in other tissues although the levels are very low compared to that in the testis. Western blot analysis of whole tissue extracts showed the presence of AR protein in fore brain, heart, and testis. The AR gene is present as a single copy in bullfrog based on Southern blot analysis of genomic DNA. Altogether, the results suggest that the bullfrog AR is evolutionary conserved and may have functions similar to those shown in other species.

AB - We have cloned and characterized a full-length cDNA of androgen receptor (AR) from the testis of bullfrog, Rana catesbeiana. The cDNA contains an open reading frame of 2328 nucleotides encoding a protein of 776 amino acid residues. The bullfrog AR shows high homology with ARs from other species in its amino acid sequence. Its overall homology with those of African clawed frog, Japanese eel and human is 70, 53, and 63%, respectively. As expected, the N-terminal domain shows much less homology (30-59%) than both DNA-binding domain (85-92%) and ligand binding domain (80-89%). Northern blot analysis detected the bullfrog AR message as a single transcript of around 9kb only in the testis. However, RT-PCR analysis revealed that AR mRNA is also expressed in other tissues although the levels are very low compared to that in the testis. Western blot analysis of whole tissue extracts showed the presence of AR protein in fore brain, heart, and testis. The AR gene is present as a single copy in bullfrog based on Southern blot analysis of genomic DNA. Altogether, the results suggest that the bullfrog AR is evolutionary conserved and may have functions similar to those shown in other species.

KW - Androgen receptor

KW - Bullfrog

KW - cDNA

UR - http://www.scopus.com/inward/record.url?scp=0042734914&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042734914&partnerID=8YFLogxK

U2 - 10.1016/S0016-6480(03)00215-6

DO - 10.1016/S0016-6480(03)00215-6

M3 - Article

C2 - 13129498

AN - SCOPUS:0042734914

VL - 134

SP - 10

EP - 17

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 1

ER -