Cloning and characterization of mouse disabled 2 interacting protein 2, a mouse orthologue of human NOSTRIN

Young Joon Choi, Si Young Cho, Hyung Wook Kim, Jung Ah Kim, Sung Ho Bae, Sung Soo Park

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The mouse disabled 2 interacting protein 2 (mDaIP2) had been obtained through yeast two hybrid system. It consists of 506 amino acids and its calculated molecular weight is 57.7 kDa. The protein contains N-terminal FCH domain and C-terminal SH3 domain. The SH3 domain interacts with the proline rich domain of mDab2 which had been identified to possess a transcriptional activation function. In RA-treated F9 teratocarcinoma cell, the mDaIP2 and mDab2 genes were differentially expressed in a RA-responsive manner and both were detected to localize in cytoplasm and nucleus. Homology search of all NCBI sequences indicated that the amino acid sequence of mDaIP2 shares 82% identity with human NOSTRIN which controls activity, trafficking, and targeting of nitric oxide synthase (eNos). The eNos was not detected in RA-treated F9 cell. These results suggest that mDaIP2 somehow functions in a different fashion from NOSTRIN in F9 cell differentiation and that its function may be concerted with that of mDab2.

Original languageEnglish
Pages (from-to)594-599
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume326
Issue number3
DOIs
Publication statusPublished - 2005 Jan 21

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Cloning
Organism Cloning
src Homology Domains
Teratocarcinoma
Amino Acids
Two-Hybrid System Techniques
Hybrid systems
Proline
Nitric Oxide Synthase
Yeast
Transcriptional Activation
Cell Differentiation
Amino Acid Sequence
Cytoplasm
Genes
Molecular Weight
Chemical activation
Molecular weight
Mouse Nostrin protein
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning and characterization of mouse disabled 2 interacting protein 2, a mouse orthologue of human NOSTRIN. / Choi, Young Joon; Cho, Si Young; Kim, Hyung Wook; Kim, Jung Ah; Bae, Sung Ho; Park, Sung Soo.

In: Biochemical and Biophysical Research Communications, Vol. 326, No. 3, 21.01.2005, p. 594-599.

Research output: Contribution to journalArticle

Choi, Young Joon ; Cho, Si Young ; Kim, Hyung Wook ; Kim, Jung Ah ; Bae, Sung Ho ; Park, Sung Soo. / Cloning and characterization of mouse disabled 2 interacting protein 2, a mouse orthologue of human NOSTRIN. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 326, No. 3. pp. 594-599.
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