Abstract
We isolated and characterized a stress-inducible gene designated as SLTI98 encoding ribosomal protein S6 in soybean. The derived amino acid sequence of SLTI98 showed the highest identity of 93% with ribosomal protein S6 from Medicago truncatula (ABD32373). The size of the full-length genomic clone of SLTI98 is 2701 bp containing 6 exons and 5 introns, of which structure is similar to that of Arabidopsis ribosomal protein S6. Genomic southern-blot analysis confirmed that soybean genome has multiple copies of the SLTI98 gene. SLTI98 RNA expression was slightly induced by salt, ABA, or wounding stress, but not by dehydration stress. The present study implies that the nuclear SLTI98 plays an important role in translational control during abiotic stresses.
| Original language | English |
|---|---|
| Pages (from-to) | 118-125 |
| Number of pages | 8 |
| Journal | Russian Journal of Plant Physiology |
| Volume | 56 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 Jan 1 |
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Keywords
- Glycine max
- Northern blot
- Ribosomal protein
- Stress
ASJC Scopus subject areas
- Plant Science
Cite this
Cloning and characterization of SLTI98 encoding ribosomal protein s6 in soybean. / Kim, K.; Chung, E.; Cho, C. W.; Soh, H. A.; Yun, B. H.; Kim, J. I.; Seo, Yong Weon; Lee, S. W.; Lee, Y. Ch; Chung, Y. S.; Lee, J. H.
In: Russian Journal of Plant Physiology, Vol. 56, No. 1, 01.01.2009, p. 118-125.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and characterization of SLTI98 encoding ribosomal protein s6 in soybean
AU - Kim, K.
AU - Chung, E.
AU - Cho, C. W.
AU - Soh, H. A.
AU - Yun, B. H.
AU - Kim, J. I.
AU - Seo, Yong Weon
AU - Lee, S. W.
AU - Lee, Y. Ch
AU - Chung, Y. S.
AU - Lee, J. H.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - We isolated and characterized a stress-inducible gene designated as SLTI98 encoding ribosomal protein S6 in soybean. The derived amino acid sequence of SLTI98 showed the highest identity of 93% with ribosomal protein S6 from Medicago truncatula (ABD32373). The size of the full-length genomic clone of SLTI98 is 2701 bp containing 6 exons and 5 introns, of which structure is similar to that of Arabidopsis ribosomal protein S6. Genomic southern-blot analysis confirmed that soybean genome has multiple copies of the SLTI98 gene. SLTI98 RNA expression was slightly induced by salt, ABA, or wounding stress, but not by dehydration stress. The present study implies that the nuclear SLTI98 plays an important role in translational control during abiotic stresses.
AB - We isolated and characterized a stress-inducible gene designated as SLTI98 encoding ribosomal protein S6 in soybean. The derived amino acid sequence of SLTI98 showed the highest identity of 93% with ribosomal protein S6 from Medicago truncatula (ABD32373). The size of the full-length genomic clone of SLTI98 is 2701 bp containing 6 exons and 5 introns, of which structure is similar to that of Arabidopsis ribosomal protein S6. Genomic southern-blot analysis confirmed that soybean genome has multiple copies of the SLTI98 gene. SLTI98 RNA expression was slightly induced by salt, ABA, or wounding stress, but not by dehydration stress. The present study implies that the nuclear SLTI98 plays an important role in translational control during abiotic stresses.
KW - Glycine max
KW - Northern blot
KW - Ribosomal protein
KW - Stress
UR - http://www.scopus.com/inward/record.url?scp=58349086588&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58349086588&partnerID=8YFLogxK
U2 - 10.1134/S1021443709010178
DO - 10.1134/S1021443709010178
M3 - Article
AN - SCOPUS:58349086588
VL - 56
SP - 118
EP - 125
JO - Russian Journal of Plant Physiology
JF - Russian Journal of Plant Physiology
SN - 1021-4437
IS - 1
ER -