Cloning and characterization of SLTI98 encoding ribosomal protein s6 in soybean

K. Kim; E. Chung; C. W. Cho; H. A. Soh; B. H. Yun; J. I. Kim; Yong Weon Seo; S. W. Lee; Y. Ch Lee; Y. S. Chung; J. H. Lee

doi:10.1134/S1021443709010178

Cloning and characterization of SLTI98 encoding ribosomal protein s6 in soybean

K. Kim, E. Chung, C. W. Cho, H. A. Soh, B. H. Yun, J. I. Kim, Yong Weon Seo, S. W. Lee, Y. Ch Lee, Y. S. Chung, J. H. Lee

Division of Biotechnology

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Abstract

We isolated and characterized a stress-inducible gene designated as SLTI98 encoding ribosomal protein S6 in soybean. The derived amino acid sequence of SLTI98 showed the highest identity of 93% with ribosomal protein S6 from Medicago truncatula (ABD32373). The size of the full-length genomic clone of SLTI98 is 2701 bp containing 6 exons and 5 introns, of which structure is similar to that of Arabidopsis ribosomal protein S6. Genomic southern-blot analysis confirmed that soybean genome has multiple copies of the SLTI98 gene. SLTI98 RNA expression was slightly induced by salt, ABA, or wounding stress, but not by dehydration stress. The present study implies that the nuclear SLTI98 plays an important role in translational control during abiotic stresses.

Original language	English
Pages (from-to)	118-125
Number of pages	8
Journal	Russian Journal of Plant Physiology
Volume	56
Issue number	1
DOIs	https://doi.org/10.1134/S1021443709010178
Publication status	Published - 2009 Jan 1

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Keywords

Glycine max
Northern blot
Ribosomal protein
Stress

ASJC Scopus subject areas

Plant Science

Cite this

Kim, K., Chung, E., Cho, C. W., Soh, H. A., Yun, B. H., Kim, J. I., Seo, Y. W., Lee, S. W., Lee, Y. C., Chung, Y. S., & Lee, J. H. (2009). Cloning and characterization of SLTI98 encoding ribosomal protein s6 in soybean. Russian Journal of Plant Physiology, 56(1), 118-125. https://doi.org/10.1134/S1021443709010178

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title = "Cloning and characterization of SLTI98 encoding ribosomal protein s6 in soybean",

abstract = "We isolated and characterized a stress-inducible gene designated as SLTI98 encoding ribosomal protein S6 in soybean. The derived amino acid sequence of SLTI98 showed the highest identity of 93% with ribosomal protein S6 from Medicago truncatula (ABD32373). The size of the full-length genomic clone of SLTI98 is 2701 bp containing 6 exons and 5 introns, of which structure is similar to that of Arabidopsis ribosomal protein S6. Genomic southern-blot analysis confirmed that soybean genome has multiple copies of the SLTI98 gene. SLTI98 RNA expression was slightly induced by salt, ABA, or wounding stress, but not by dehydration stress. The present study implies that the nuclear SLTI98 plays an important role in translational control during abiotic stresses.",

keywords = "Glycine max, Northern blot, Ribosomal protein, Stress",

author = "K. Kim and E. Chung and Cho, {C. W.} and Soh, {H. A.} and Yun, {B. H.} and Kim, {J. I.} and Seo, {Yong Weon} and Lee, {S. W.} and Lee, {Y. Ch} and Chung, {Y. S.} and Lee, {J. H.}",

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T1 - Cloning and characterization of SLTI98 encoding ribosomal protein s6 in soybean

AU - Kim, K.

AU - Chung, E.

AU - Cho, C. W.

AU - Soh, H. A.

AU - Yun, B. H.

AU - Kim, J. I.

AU - Seo, Yong Weon

AU - Lee, S. W.

AU - Lee, Y. Ch

AU - Chung, Y. S.

AU - Lee, J. H.

PY - 2009/1/1

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N2 - We isolated and characterized a stress-inducible gene designated as SLTI98 encoding ribosomal protein S6 in soybean. The derived amino acid sequence of SLTI98 showed the highest identity of 93% with ribosomal protein S6 from Medicago truncatula (ABD32373). The size of the full-length genomic clone of SLTI98 is 2701 bp containing 6 exons and 5 introns, of which structure is similar to that of Arabidopsis ribosomal protein S6. Genomic southern-blot analysis confirmed that soybean genome has multiple copies of the SLTI98 gene. SLTI98 RNA expression was slightly induced by salt, ABA, or wounding stress, but not by dehydration stress. The present study implies that the nuclear SLTI98 plays an important role in translational control during abiotic stresses.

AB - We isolated and characterized a stress-inducible gene designated as SLTI98 encoding ribosomal protein S6 in soybean. The derived amino acid sequence of SLTI98 showed the highest identity of 93% with ribosomal protein S6 from Medicago truncatula (ABD32373). The size of the full-length genomic clone of SLTI98 is 2701 bp containing 6 exons and 5 introns, of which structure is similar to that of Arabidopsis ribosomal protein S6. Genomic southern-blot analysis confirmed that soybean genome has multiple copies of the SLTI98 gene. SLTI98 RNA expression was slightly induced by salt, ABA, or wounding stress, but not by dehydration stress. The present study implies that the nuclear SLTI98 plays an important role in translational control during abiotic stresses.

KW - Glycine max

KW - Northern blot

KW - Ribosomal protein

KW - Stress

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10.1134/S1021443709010178