Cloning and characterization of the chloroplast elongation factor EF-Tu cDNA of Oryza sativa L.

Jeong Hwan Lee, Joong Won Lee, Yong Yoon Chung, Kyung Hee Paek, Jeong Sheop Shin, Choong Hyo Yun, Jeong Kook Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

From the rice leaf cDNA library, we have cloned a cDNA encoding rice chloroplast translational elongation factor EF-Tu (tufA). The rice tufA cDNA clone contains 1678 nucleotides and codes for a 467 amino acid protein including a putative chloroplast transit peptide of 59 amino acid residues. The predicted molecular mass of the mature protein is approximately 45 kDa. This cDNA clone contains the 61 nucleotides of the 5′ untranslated region (UTR) and the 213 nucleotides of 3′ UTR. Amino acid sequence identity of the rice tufA with the mature chloroplast EF-Tu proteins of tobacco, pea, arabidopsis, and soybean ranges from 83% to 86%. The deduced polypeptide of the rice tufA cDNA contains GTP binding domains in its N-terminal region and chloroplast EF-Tu signature regions in the C-terminal region. The rice tufA appears to exist as a single copy gene, although its homologues of maize and oat exist as multiple copy genes. The rice tufA gene is located in chromosome 1 and is more highly expressed in the leaf than in root tissue.

Original languageEnglish
Pages (from-to)484-490
Number of pages7
JournalMolecules and Cells
Volume9
Issue number5
Publication statusPublished - 1999 Oct 31

Fingerprint

Peptide Elongation Factor Tu
Chloroplasts
Organism Cloning
Complementary DNA
Nucleotides
Clone Cells
Genes
Amino Acids
Proteins
5' Untranslated Regions
Chromosomes, Human, Pair 1
Peas
3' Untranslated Regions
Guanosine Triphosphate
Oryza
Gene Library
Soybeans
Arabidopsis
Zea mays
Tobacco

Keywords

  • Chloroplast
  • Elongation Factor Tu
  • Gene Families
  • Rice
  • Signature Regions

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Cloning and characterization of the chloroplast elongation factor EF-Tu cDNA of Oryza sativa L. / Lee, Jeong Hwan; Lee, Joong Won; Chung, Yong Yoon; Paek, Kyung Hee; Shin, Jeong Sheop; Yun, Choong Hyo; Kim, Jeong Kook.

In: Molecules and Cells, Vol. 9, No. 5, 31.10.1999, p. 484-490.

Research output: Contribution to journalArticle

Lee, Jeong Hwan ; Lee, Joong Won ; Chung, Yong Yoon ; Paek, Kyung Hee ; Shin, Jeong Sheop ; Yun, Choong Hyo ; Kim, Jeong Kook. / Cloning and characterization of the chloroplast elongation factor EF-Tu cDNA of Oryza sativa L. In: Molecules and Cells. 1999 ; Vol. 9, No. 5. pp. 484-490.
@article{06f47bd6ea5b4065b0ad785b194c076d,
title = "Cloning and characterization of the chloroplast elongation factor EF-Tu cDNA of Oryza sativa L.",
abstract = "From the rice leaf cDNA library, we have cloned a cDNA encoding rice chloroplast translational elongation factor EF-Tu (tufA). The rice tufA cDNA clone contains 1678 nucleotides and codes for a 467 amino acid protein including a putative chloroplast transit peptide of 59 amino acid residues. The predicted molecular mass of the mature protein is approximately 45 kDa. This cDNA clone contains the 61 nucleotides of the 5′ untranslated region (UTR) and the 213 nucleotides of 3′ UTR. Amino acid sequence identity of the rice tufA with the mature chloroplast EF-Tu proteins of tobacco, pea, arabidopsis, and soybean ranges from 83{\%} to 86{\%}. The deduced polypeptide of the rice tufA cDNA contains GTP binding domains in its N-terminal region and chloroplast EF-Tu signature regions in the C-terminal region. The rice tufA appears to exist as a single copy gene, although its homologues of maize and oat exist as multiple copy genes. The rice tufA gene is located in chromosome 1 and is more highly expressed in the leaf than in root tissue.",
keywords = "Chloroplast, Elongation Factor Tu, Gene Families, Rice, Signature Regions",
author = "Lee, {Jeong Hwan} and Lee, {Joong Won} and Chung, {Yong Yoon} and Paek, {Kyung Hee} and Shin, {Jeong Sheop} and Yun, {Choong Hyo} and Kim, {Jeong Kook}",
year = "1999",
month = "10",
day = "31",
language = "English",
volume = "9",
pages = "484--490",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "5",

}

TY - JOUR

T1 - Cloning and characterization of the chloroplast elongation factor EF-Tu cDNA of Oryza sativa L.

AU - Lee, Jeong Hwan

AU - Lee, Joong Won

AU - Chung, Yong Yoon

AU - Paek, Kyung Hee

AU - Shin, Jeong Sheop

AU - Yun, Choong Hyo

AU - Kim, Jeong Kook

PY - 1999/10/31

Y1 - 1999/10/31

N2 - From the rice leaf cDNA library, we have cloned a cDNA encoding rice chloroplast translational elongation factor EF-Tu (tufA). The rice tufA cDNA clone contains 1678 nucleotides and codes for a 467 amino acid protein including a putative chloroplast transit peptide of 59 amino acid residues. The predicted molecular mass of the mature protein is approximately 45 kDa. This cDNA clone contains the 61 nucleotides of the 5′ untranslated region (UTR) and the 213 nucleotides of 3′ UTR. Amino acid sequence identity of the rice tufA with the mature chloroplast EF-Tu proteins of tobacco, pea, arabidopsis, and soybean ranges from 83% to 86%. The deduced polypeptide of the rice tufA cDNA contains GTP binding domains in its N-terminal region and chloroplast EF-Tu signature regions in the C-terminal region. The rice tufA appears to exist as a single copy gene, although its homologues of maize and oat exist as multiple copy genes. The rice tufA gene is located in chromosome 1 and is more highly expressed in the leaf than in root tissue.

AB - From the rice leaf cDNA library, we have cloned a cDNA encoding rice chloroplast translational elongation factor EF-Tu (tufA). The rice tufA cDNA clone contains 1678 nucleotides and codes for a 467 amino acid protein including a putative chloroplast transit peptide of 59 amino acid residues. The predicted molecular mass of the mature protein is approximately 45 kDa. This cDNA clone contains the 61 nucleotides of the 5′ untranslated region (UTR) and the 213 nucleotides of 3′ UTR. Amino acid sequence identity of the rice tufA with the mature chloroplast EF-Tu proteins of tobacco, pea, arabidopsis, and soybean ranges from 83% to 86%. The deduced polypeptide of the rice tufA cDNA contains GTP binding domains in its N-terminal region and chloroplast EF-Tu signature regions in the C-terminal region. The rice tufA appears to exist as a single copy gene, although its homologues of maize and oat exist as multiple copy genes. The rice tufA gene is located in chromosome 1 and is more highly expressed in the leaf than in root tissue.

KW - Chloroplast

KW - Elongation Factor Tu

KW - Gene Families

KW - Rice

KW - Signature Regions

UR - http://www.scopus.com/inward/record.url?scp=0033621206&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033621206&partnerID=8YFLogxK

M3 - Article

C2 - 10597036

AN - SCOPUS:0033621206

VL - 9

SP - 484

EP - 490

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 5

ER -