Cloning and characterization of the new multiple stress responsible gene i (MuSI) from sweet potato

Sang Gyu Seo, Ji Seoung Kim, Yu Sun Yang, Byung Ki Jun, Seung Won Kang, Gung Pyo Lee, Wook Kim, Jong Bo Kim, Hyeong Un Lee, Sun Hyung Kim

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

New multiple-stress related gene isolated from sweet potato and designated it as MusI (multiple stress responsible gene I). Sequence analysis revealed that its full length cDNA was 998 bp long and included a 717 bp open reading frame encoding for 238 amino acids. Comparison of its cDNA and genomic DNA sequence showed that 3 exons were divided by 2 introns in its ORF region. Its deduced amino acid sequence contained a conserved rubber elongation factor (REF) domain and showed high homology with many stress-related proteins. Therefore, it was named MuSI (multiple stress responsible gene I). Southern hybridization analysis indicated that the MuSI gene may belong to a multi-gene family. Expression pattern of the MuSI gene showed that it was differently expressed among roots, stems, leaves, and flowers of a sweet potato, and its expression level was especially high in flowers and white fibrous roots. Its expression was also highly induced by various stress signals including dehydration, high salt, heavy metal, oxidation, and plant hormones. Stress tolerance experiment using transgenic plants overexpressing the MuSI gene showed that all independent transgenic tobacco lines have enhanced tolerance to high temperature stress. Among them, transgenic line 6 particularly showed tolerance to salt, heavy metal, and osmotic stress as well. These results suggest that the MuSI gene functions as a positive regulator of various stress responses and may be useful in improving stress tolerance of transgenic plants.

Original languageEnglish
Pages (from-to)544-552
Number of pages9
JournalGenes and Genomics
Volume32
Issue number6
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Ipomoea batatas
Cloning
Organism Cloning
Genes
Genetically Modified Plants
Heavy Metals
Open Reading Frames
Complementary DNA
Salts
Peptide Elongation Factors
Amino Acids
Plant Growth Regulators
Tobacco
DNA sequences
Rubber
Osmotic Pressure
Heat-Shock Proteins
Dehydration
Introns
Sequence Analysis

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Molecular Biology

Cite this

Seo, S. G., Kim, J. S., Yang, Y. S., Jun, B. K., Kang, S. W., Lee, G. P., ... Kim, S. H. (2014). Cloning and characterization of the new multiple stress responsible gene i (MuSI) from sweet potato. Genes and Genomics, 32(6), 544-552. https://doi.org/10.1007/s13258-010-0093-7

Cloning and characterization of the new multiple stress responsible gene i (MuSI) from sweet potato. / Seo, Sang Gyu; Kim, Ji Seoung; Yang, Yu Sun; Jun, Byung Ki; Kang, Seung Won; Lee, Gung Pyo; Kim, Wook; Kim, Jong Bo; Lee, Hyeong Un; Kim, Sun Hyung.

In: Genes and Genomics, Vol. 32, No. 6, 01.01.2014, p. 544-552.

Research output: Contribution to journalArticle

Seo, SG, Kim, JS, Yang, YS, Jun, BK, Kang, SW, Lee, GP, Kim, W, Kim, JB, Lee, HU & Kim, SH 2014, 'Cloning and characterization of the new multiple stress responsible gene i (MuSI) from sweet potato', Genes and Genomics, vol. 32, no. 6, pp. 544-552. https://doi.org/10.1007/s13258-010-0093-7
Seo, Sang Gyu ; Kim, Ji Seoung ; Yang, Yu Sun ; Jun, Byung Ki ; Kang, Seung Won ; Lee, Gung Pyo ; Kim, Wook ; Kim, Jong Bo ; Lee, Hyeong Un ; Kim, Sun Hyung. / Cloning and characterization of the new multiple stress responsible gene i (MuSI) from sweet potato. In: Genes and Genomics. 2014 ; Vol. 32, No. 6. pp. 544-552.
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