Cloning and expression analysis of chicken phospholipid-hydroperoxide glutathione peroxidase

Byung W. Kong, Hyunggee Kim, Douglas N. Foster

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Phospholipid-hydroperoxide glutathione peroxidase (GPX4 or PHGPX) is a unique selenium dependent glutathione peroxidase that reduces phospholipid, cholesterol, and cholesteryl ester hydroperoxides. Phospholipid-hydroperoxide glutathione peroxidase has been shown to exist as both a 197 amino acid mitochondrial targeting protein and as a 170 amino acid non-mitochondrial protein. The cDNA encoding the non-mitochondrial chicken GPX4 (cGPX4) has been isolated from an immortalized DF-1 chicken embryonic fibroblast (CEF) cell line cDNA library. The nucleotide sequence of cGPX4 was 802 bp in length with an open reading frame (ORF) that encoded 170 amino acids but lacked the N-terminal domain that encoded the mitochondrial leader sequence (MLS). Chicken non-mitochondrial GPX4 was highly expressed in brain and stromal tissues. Surprisingly, it was found that ovarian stromal tissue cGPX4 expression is regulated quite differently according to the reproductive status of the bird, suggesting that GPX4 may play an important role in reproduction in response to steroid hormones, in addition to its general antioxidant functions.

Original languageEnglish
Pages (from-to)19-29
Number of pages11
JournalAnimal Biotechnology
Volume14
Issue number1
DOIs
Publication statusPublished - 2003 May 1
Externally publishedYes

Fingerprint

phospholipid-hydroperoxide glutathione peroxidase
Cloning
Phospholipids
Amino acids
Organism Cloning
molecular cloning
Chickens
chickens
Amino Acids
Steroid hormones
cholesteryl esters
Tissue
Proteins
Cholesterol Esters
Cholesterol
amino acids
Selenium
Birds
Fibroblasts
Nucleotides

Keywords

  • Antioxidant
  • Chicken
  • PHGPX
  • Reproductive cycle expression of PHGPX
  • Selenium dependent protein

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biotechnology
  • Bioengineering

Cite this

Cloning and expression analysis of chicken phospholipid-hydroperoxide glutathione peroxidase. / Kong, Byung W.; Kim, Hyunggee; Foster, Douglas N.

In: Animal Biotechnology, Vol. 14, No. 1, 01.05.2003, p. 19-29.

Research output: Contribution to journalArticle

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