Cloning and expression of inulin fructotransferase gene of Arthrobacter sp. A-6 in Escherichia coli and Bacillus subtilis

H. Y. Kim, C. W. Kim, Y. J. Cho

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The inulin fructotransferase (depolymerizing) (IFTase, EC 2.4.1.93) gene of Arthrobacter sp. A-6 was cloned and expressed in Escherichia coli and Bacillus subtilis. The IFTase gene consisted of an ORF of 1,311 nucleotides encoding a polypeptide of 436 amino acids containing a signal peptide of 31 amino acids in the N-terminus. The molecular mass of the IFTase based on the nucleotide sequence was calculated to be 46,116 Da. The recombinant E. coli DH5α cells expressing the Arthrobacter sp. A-6 IFTase gene produced most of the IFTase intracellularly. In contrast, the recombinant B. subtilis DB 104 carrying the IFrase gene on a B. subtilis-E. coli expression vector secreted the IFrase into the culture fluid efficiently.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalJournal of microbiology and biotechnology
Volume10
Issue number2
Publication statusPublished - 2000

Keywords

  • Arthrobacter
  • Bacillus expression
  • DFA
  • Difructofuranose
  • Inulin fructotransferase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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