Cloning and expression of male-specific protein (MSP) from the hemolymph of greater wax moth, Galleria mellonella L.

Jikhyon Han, Chang Seok Lee, Chi Young Yun, Bong Hee Lee, Young-Gyu Ko, Chang Soo Kang, Sang Dae Lee, Jae Sam Hwang, Seok Woo Kang, Hak R. Kim

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4 Citations (Scopus)

Abstract

Male-specific protein (MSP) is a soluble protein that accumulates in high amounts in the hemolymph and other organs of adult male wax moth. The MSP was purified from adult male wax moth by gel filtration and reversed phase column chromatography, and its amino acid sequence was determined. Because of blocked N-terminus, several internal amino acid sequences of MSP were obtained by the in-gel digestion method using trypsin. RT-PCR was conducted using degenerate primers designed from the internal amino add sequences. 5′-RACE PCR was used to obtain the complete coding region and 5′-UTR sequence. The full length MSP cDNA sequence encodes a 239 amino acid polypeptide with an 18 amino acid signal peptide. The putative mature MSP has a molecular mass of 24,317 Da and an isoelectric point (pl) of 6.00, but shows a molecular mass of 27 kDa on SDS-PAGE. Sequence alignment showed a significant similarity between MSP and juvenile hormone binding proteins (JHBPs) of several lepidopteran species, including G. mellonella.

Original languageEnglish
Pages (from-to)110-120
Number of pages11
JournalArchives of Insect Biochemistry and Physiology
Volume54
Issue number3
DOIs
Publication statusPublished - 2003 Nov 1

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Keywords

  • cDNA
  • Galleria mellonella
  • Juvenile hormone binding protein
  • Mole-specific protein
  • MSP

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physiology
  • Physiology (medical)

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