Cloning and expression of superoxide dismutase from Aquifex pyrophilus, a hyperthermophilic bacterium

Jae Hwan Lim, Yeon Gyu Yu, In-Geol Choi, Jae Ryeon Ryu, Byung-Yoon Ahn, Sung H. Kim, Ye Sun Han

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A superoxide dismutase (SOD) gene of Aquifex pyrophilus, a marine hyperthermophilic bacterium, was cloned, sequenced, expressed in Escherichia coli, and its gene product characterized. This is the first SOD from a hyperthermophilic bacterium that has been cloned. It is an iron-containing homooligomeric protein with a monomeric molecular mass of 24.2 kDa. The DNA-derived amino acid sequence is more similar to those of known Mn- and Fe-SODs from thermophilic archaea than of Cu, Zn-SODs. The metal binding residues found in all SOD sequences from different species are also conserved in A. pyrophilus SOD. The protein is biochemically active only as an oligomer and is resistant to thermal denaturation.

Original languageEnglish
Pages (from-to)142-146
Number of pages5
JournalFEBS Letters
Volume406
Issue number1-2
DOIs
Publication statusPublished - 1997 Apr 7

Fingerprint

Cloning
Superoxide Dismutase
Organism Cloning
Bacteria
Genes
Denaturation
Molecular mass
Oligomers
Escherichia coli
Archaea
Proteins
Iron
Metals
Amino Acid Sequence
Amino Acids
Hot Temperature
DNA

Keywords

  • Aquifex
  • Hyperthermophile
  • Superoxide dismutase
  • Thermostability

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning and expression of superoxide dismutase from Aquifex pyrophilus, a hyperthermophilic bacterium. / Lim, Jae Hwan; Yu, Yeon Gyu; Choi, In-Geol; Ryu, Jae Ryeon; Ahn, Byung-Yoon; Kim, Sung H.; Han, Ye Sun.

In: FEBS Letters, Vol. 406, No. 1-2, 07.04.1997, p. 142-146.

Research output: Contribution to journalArticle

Lim, Jae Hwan ; Yu, Yeon Gyu ; Choi, In-Geol ; Ryu, Jae Ryeon ; Ahn, Byung-Yoon ; Kim, Sung H. ; Han, Ye Sun. / Cloning and expression of superoxide dismutase from Aquifex pyrophilus, a hyperthermophilic bacterium. In: FEBS Letters. 1997 ; Vol. 406, No. 1-2. pp. 142-146.
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