Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain

Pom Joo Lee, Ji Young Ahn, Yang Hoon Kim, Seung Wook Kim, Ji Yeon Kim, Jae Sung Park, Jeewon Lee

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have identified and cloned a novel toxin gene (tccC1/xptB1) from Xenorhabdus nematophilus strain isolated from Korea-specific entomophagous nematode Steinernema glaseri MK. The DNA sequence of cloned toxin gene (3048bp) has an open reading frame encoding 1016 amino acids with a predicted molecular mass of 111058Da. The toxin sequence shares 50-96% identical amino acid residues with the previously reported tccC1 cloned from X. nematophilus (AJ308438), Photorhabdus luminescens W14 (AF346499) P. luminescens TTO1 (BX571873), and Yersinia pestis CO92 (NC_003143). The toxin gene was successfully expressed in Escherichia coli, and the recombinant toxin protein caused a rapid cessation in mortality of Galleria mellonella larvae (80% death of larvae within 2 days). Conclusively, the heterologous expression of the novel gene tccC1 cloned into E. coli plasmid vector produced recombinant toxin with high insecticidal activity.

Original languageEnglish
Pages (from-to)1110-1116
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume319
Issue number4
DOIs
Publication statusPublished - 2004 Jul 9

Fingerprint

Xenorhabdus
Cloning
Photorhabdus
Organism Cloning
Genes
Larva
Escherichia coli
Yersinia pestis
Amino Acids
Korea
Recombinant Proteins
Recombinant proteins
Open Reading Frames
DNA sequences
Molecular mass
Plasmids
Gene Expression
Mortality

Keywords

  • Heterologous expression
  • High insecticidal activity
  • Toxin gene (tccC1/XptB1)
  • Xenorhabdus nematophilus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain. / Lee, Pom Joo; Ahn, Ji Young; Kim, Yang Hoon; Kim, Seung Wook; Kim, Ji Yeon; Park, Jae Sung; Lee, Jeewon.

In: Biochemical and Biophysical Research Communications, Vol. 319, No. 4, 09.07.2004, p. 1110-1116.

Research output: Contribution to journalArticle

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