Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain

Pom Joo Lee, Ji Young Ahn, Yang Hoon Kim, Seung Wook Kim, Ji Yeon Kim, Jae Sung Park, Jeewon Lee

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have identified and cloned a novel toxin gene (tccC1/xptB1) from Xenorhabdus nematophilus strain isolated from Korea-specific entomophagous nematode Steinernema glaseri MK. The DNA sequence of cloned toxin gene (3048bp) has an open reading frame encoding 1016 amino acids with a predicted molecular mass of 111058Da. The toxin sequence shares 50-96% identical amino acid residues with the previously reported tccC1 cloned from X. nematophilus (AJ308438), Photorhabdus luminescens W14 (AF346499) P. luminescens TTO1 (BX571873), and Yersinia pestis CO92 (NC_003143). The toxin gene was successfully expressed in Escherichia coli, and the recombinant toxin protein caused a rapid cessation in mortality of Galleria mellonella larvae (80% death of larvae within 2 days). Conclusively, the heterologous expression of the novel gene tccC1 cloned into E. coli plasmid vector produced recombinant toxin with high insecticidal activity.

Original languageEnglish
Pages (from-to)1110-1116
Number of pages7
JournalBiochemical and biophysical research communications
Volume319
Issue number4
DOIs
Publication statusPublished - 2004 Jul 9

Keywords

  • Heterologous expression
  • High insecticidal activity
  • Toxin gene (tccC1/XptB1)
  • Xenorhabdus nematophilus

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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