Cloning and localization of rgpr85 encoding rat G-protein-coupled receptor

Jongrye Jeon, Changmee Kim, Woong Sun, Hyesun Chung, Sun Hwa Park, Hyun Kim

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

In an attempt to isolate genes involved in the brain development using ordered differential display PCR, we cloned rgpr85 which encodes rat G-protein-coupled receptor with high degree of identity to the amine-like neurotransmitter receptors. This gene was found to be localized at rat chromosome 4q21. In situ hybridization demonstrated that rgpr85 was predominantly expressed in the developing brain and spinal cord. Hybridization signal was especially abundant within the embryonic cortical plates where post-mitotic cortical neurons are localized. In the cerebral cortex, the expression of rgpr85 was gradually decreased postnatally and became undetectable by P18. However, weak but significant expression of rgpr85 was maintained in the adult hippocampal formation, olfactory bulb, and cerebellum. Interestingly, rgpr85 expression was transiently induced in the adult hippocampal formation, piriform cortex, and amygdaloid complex by kainic acid (KA) treatment. Thus, dynamic regulation of rgpr85 expression suggests an importance of rgpr85-mediated signaling in the development of cerebral cortex and in the KA-induced responses in the adult brain.

Original languageEnglish
Pages (from-to)613-618
Number of pages6
JournalBiochemical and biophysical research communications
Volume298
Issue number4
DOIs
Publication statusPublished - 2002

Keywords

  • Development of cerebral cortex
  • In situ hybridization
  • Kainic acid
  • ODD-PCR
  • Rat chromosome 4q21
  • Rgpr85

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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