Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25

Young Hee Jung; Jong Ok Ka; Choong Ill Cheon; Myeong Sok Lee; Eun Sook Song; Soon Young Choi; Daeho Cho; Sang Ho Choi; Kwon Soo Ha; Young Mok Park; Jong Soon Choi; Kyung Hee Min

Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25

Young Hee Jung, Jong Ok Ka, Choong Ill Cheon, Myeong Sok Lee, Eun Sook Song, Soon Young Choi, Daeho Cho, Sang Ho Choi, Kwon Soo Ha, Young Mok Park, Jong Soon Choi, Kyung Hee Min

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the vector pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed active-site region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, they could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RB1.

Original language	English
Pages (from-to)	102-108
Number of pages	7
Journal	Journal of Microbiology
Volume	41
Issue number	2
Publication status	Published - 2003 Jun
Externally published	Yes

Keywords

Catechol degradation
Cloning and sequence analysis
catB and catC genes

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

Cite this

@article{27067a2b668f416aa7b2efd3b3b9eabe,

title = "Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25",

abstract = "A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the vector pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed active-site region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, they could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RB1.",

keywords = "Catechol degradation, Cloning and sequence analysis, catB and catC genes",

author = "Jung, {Young Hee} and Ka, {Jong Ok} and Cheon, {Choong Ill} and Lee, {Myeong Sok} and Song, {Eun Sook} and Choi, {Soon Young} and Daeho Cho and Choi, {Sang Ho} and Ha, {Kwon Soo} and Park, {Young Mok} and Choi, {Jong Soon} and Min, {Kyung Hee}",

year = "2003",

month = jun,

language = "English",

volume = "41",

pages = "102--108",

journal = "Journal of Microbiology",

issn = "1225-8873",

publisher = "Microbiological Society of Korea",

number = "2",

}

TY - JOUR

T1 - Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25

AU - Jung, Young Hee

AU - Ka, Jong Ok

AU - Cheon, Choong Ill

AU - Lee, Myeong Sok

AU - Song, Eun Sook

AU - Choi, Soon Young

AU - Cho, Daeho

AU - Choi, Sang Ho

AU - Ha, Kwon Soo

AU - Park, Young Mok

AU - Choi, Jong Soon

AU - Min, Kyung Hee

PY - 2003/6

Y1 - 2003/6

N2 - A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the vector pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed active-site region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, they could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RB1.

AB - A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the vector pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed active-site region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, they could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RB1.

KW - Catechol degradation

KW - Cloning and sequence analysis

KW - catB and catC genes

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M3 - Article

AN - SCOPUS:0038796252

SN - 1225-8873

VL - 41

SP - 102

EP - 108

JO - Journal of Microbiology

JF - Journal of Microbiology

IS - 2

ER -

Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25

Abstract

Keywords

ASJC Scopus subject areas

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