Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25

Young Hee Jung, Jong Ok Ka, Choong Ill Cheon, Myeong Sok Lee, Eun Sook Song, Soon Young Choi, Daeho Cho, Sang Ho Choi, Kwon Soo Ha, Young Mok Park, Jong Soon Choi, Kyung Hee Min

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A 6.1 kb Sph I fragment from the genomic DNA of Pseudomonas putida SM 25 was cloned into the vector pUC19. The open reading frame of catB was found to consist of 1,122 nucleotides. The sequence alignment of the catB gene products from different kinds of bacteria revealed an overall identity ranging from 40 to 98%. The catC gene contained an open reading frame of 96 codons, from which a protein with a molecular mass of about 10.6 kDa was predicted. The amino acids in the proposed active-site region of CatC were found to be almost conserved, including the charged residues. Since the catBC genes in P. putida SM25 were tightly linked, they could be regulated under coordinate transcription, and transcribed from a single promoter located upstream of the catB gene, as in P. putida RB1.

Original languageEnglish
Pages (from-to)102-108
Number of pages7
JournalJournal of Microbiology
Volume41
Issue number2
Publication statusPublished - 2003 Jun 1

Keywords

  • Catechol degradation
  • Cloning and sequence analysis
  • catB and catC genes

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25'. Together they form a unique fingerprint.

  • Cite this

    Jung, Y. H., Ka, J. O., Cheon, C. I., Lee, M. S., Song, E. S., Choi, S. Y., Cho, D., Choi, S. H., Ha, K. S., Park, Y. M., Choi, J. S., & Min, K. H. (2003). Cloning and sequence analysis of two catechol-degrading gene clusters from a phenol-utilizing bacterium Pseudomonas putida SM25. Journal of Microbiology, 41(2), 102-108.