Cloning and Sequencing Analysis of cadC Encoding Transcriptional Activator CadC from Salmonella typhimurium

Bae Hoon Kim, Ho Jeong Lee, In Soo Lee, Sung Ho Bang, Joon Kim, Yong Keun Park

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Salmonella typhimurium possesses a cad operon, which contributes to an adaptive response against an acidifying environment. In Escherichia coli, the activation of the cad operon is dependent on cadC, which is located upstream of the operon. However, the activator of cad operon in S. typhimurium has not been known until now. In this study, we selected a putative cadC mutant by trasposon mutagenesis and cloned the cadC of S. typhimurium. Moreover, the cadC mutant was complemented by cadC clone. The cadC gene from S. typhimurium LT-2 consists of 1539 bp encoding a polypeptide ob 512 amino acids, and shows sequence similarity to cadC of E. coli with 53% identity and 67% similarity. The hydrophobicity profile of the S. typhimurim CadC sequence is very similar to E. coli CadC.

Original languageEnglish
Pages (from-to)109-115
Number of pages7
JournalJournal of Microbiology
Volume39
Issue number2
Publication statusPublished - 2001 Dec 1

Fingerprint

Salmonella typhimurium
Operon
Organism Cloning
Escherichia coli
Hydrophobic and Hydrophilic Interactions
Mutagenesis
Amino Acid Sequence
Clone Cells
Peptides
Genes

Keywords

  • Acd
  • Acidifying
  • Adaptive response
  • Cad operon
  • Samonella typhimurium

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Cloning and Sequencing Analysis of cadC Encoding Transcriptional Activator CadC from Salmonella typhimurium. / Kim, Bae Hoon; Lee, Ho Jeong; Lee, In Soo; Bang, Sung Ho; Kim, Joon; Park, Yong Keun.

In: Journal of Microbiology, Vol. 39, No. 2, 01.12.2001, p. 109-115.

Research output: Contribution to journalArticle

Kim, Bae Hoon ; Lee, Ho Jeong ; Lee, In Soo ; Bang, Sung Ho ; Kim, Joon ; Park, Yong Keun. / Cloning and Sequencing Analysis of cadC Encoding Transcriptional Activator CadC from Salmonella typhimurium. In: Journal of Microbiology. 2001 ; Vol. 39, No. 2. pp. 109-115.
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